Development of an alternative approach for detecting botulinum neurotoxin type A in honey: Analysis of non-toxic peptides with a reference labelled protein via liquid chromatography-tandem mass spectrometry

Koike, Hiroshi; Kanda, Manabu; Hayashi, Hairoshi; Matsushima, Yoko; Yoshikawa, Souichi; Ohba, Yumi; Hayashi, Momoka; Nagano, Chieko; Sekimura, Kotaro; Otsuka, Kenji; Kamiie, Junichi; Sasamoto, Takeo; Hashimoto, Tsuneo

doi:10.1080/19440049.2020.1766121

Cited by 4 publications

(6 citation statements)

References 41 publications

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“…This is because of the presence of the amino terminus of the N-terminal hydrophilic peptide chain of the amino acid sequence (N(S)-Ile-Leu-Arg-Gly-Glu-Ser-Leu-Asn-Lys-Ser-Leu-Pro-Ile-Leu) [37]. The most prevalent amino acids in honey were determined by analyzing forty samples of monotype honey, which included honey from ilex, oak, heather, and nut trees [40]. These amino acids were as follows: Pro, Asp, Glu, Asn, Ser, Gln, Thr, Arg, Ala, Tyr, Met, Val, Phe, Ileu, Leu, and Lys.…”

Section: Amino Acid Analysismentioning

confidence: 99%

“…These amino acids were as follows: Pro, Asp, Glu, Asn, Ser, Gln, Thr, Arg, Ala, Tyr, Met, Val, Phe, Ileu, Leu, and Lys. These amino acids were determined by employing the HPLC technique in the presence of OPA and FMOC reagents [40]. Koike et al [40], found a class of biological peptides that can be distinguished from the hydrolysis of BoNTA-derived proteins.…”

Section: Amino Acid Analysismentioning

confidence: 99%

“…These amino acids were determined by employing the HPLC technique in the presence of OPA and FMOC reagents [40]. Koike et al [40], found a class of biological peptides that can be distinguished from the hydrolysis of BoNTA-derived proteins. Because this molecule included an amino sequence in the peptide chain that translated as Leu-Tyr-Gly-Ile-Ala-Ile-Asn-Pro-Asn-Arg, the study suggested that it might be used as a distinguishing feature and a quick way for determining the purity level of honey.…”

Section: Amino Acid Analysismentioning

confidence: 99%

“…The peaks were then collected, concentrated, and kept at a temperature of −20 • C until their subsequent usage. While the non-toxic peptides originating in the honey of bees, known as BoNTA, were isolated and identified in nineteen samples from various locations using liquid chromatography procedures, they were regarded as a tool to test the product's safety [40]. Using the RP-HPLC technique on a ShimadzuLC-10 HPLC system at room temperature and using a separation column type (C18 Jupiter column, USA), Da Silva et al [61] characterized a broad spectrum of biological peptides derived from one of the beneficial bacteria.…”

Section: Pore Diametermentioning

confidence: 99%

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Current Trends and Technological Advancements in the Study of Honey Bee-Derived Peptides with an Emphasis on State-of-the-Art Approaches: A Review

Al-Rubaie,

Al-Fekaiki,

Niamah

et al. 2024

Separations

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Honey is a natural product that is used by a large number of people because of its distinctive compositional constituents, which have a considerable impact on its market value. The distinctive combination of amino acids and sugars found in honey’s composition, along with its peptide content, could potentially provide several benefits to human health. During the past few years, cutting-edge techniques have been developed and used for the purpose of investigating, identifying, and characterizing peptides that are produced from honey bees. Therefore, the purpose of this review is to examine current trends and technological advancements in the study of honey bee-derived peptides, focusing on innovative and cutting-edge methods. Furthermore, this review explores various attributes of honey and its components, including the honey bee-derived peptide defensin-1. In addition, this review investigates various methods for separating and purifying peptides, as well as the factors that affect these methods. Additionally, defensin-1, a peptide produced by honey bees, is discussed along with its antioxidant and antimicrobial capabilities. In addition, this review focuses on cutting-edge and innovative omic methods used to study honey bee peptides, as well as the significance of artificial intelligence tools in their investigation. Consequently, the review paper delves into various significant obstacles faced by researchers and scientists studying honey bee peptides, while also offering an extensive range of fascinating opportunities and possibilities for future research for those interested in groundbreaking discoveries in this area.

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Section: Amino Acid Analysismentioning

confidence: 99%

Section: Amino Acid Analysismentioning

confidence: 99%

Section: Amino Acid Analysismentioning

confidence: 99%

Section: Pore Diametermentioning

confidence: 99%

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Current Trends and Technological Advancements in the Study of Honey Bee-Derived Peptides with an Emphasis on State-of-the-Art Approaches: A Review

Al-Rubaie,

Al-Fekaiki,

Niamah

et al. 2024

Separations

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“…A liquid chromatography-tandem mass spectrometry (LC-MS/MS) alternative approach was developed to detect BoNT/A in honey, using a reference labelled protein (limit of detection 9.4 ng/mL). This method can be useful in the monitoring of food safety, and more specifically to avoid infant botulism [ 43 ]. A LC-MS/MS method coupled to an immunocapture with antibodies against BoNT/A light chain (L) allows the detection and identification of each BoNT/A subtype (limit of detection 120–150 mouse lethal doses) [ 44 ].…”

Section: Bont Detectionmentioning

confidence: 99%

Recent Developments in Botulinum Neurotoxins Detection

Rasetti-Escargueil

Popoff

2022

Microorganisms

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Botulinum neurotoxins (BoNTs) are produced as protein complexes by bacteria of the genus Clostridium that are Gram-positive, anaerobic and spore forming (Clostridium botulinum, C. butyricum, C. baratii and C. argentinense spp.). BoNTs show a high immunological and genetic diversity. Therefore, fast, precise, and more reliable detection methods are still required to monitor outbreaks and ensure surveillance of botulism. The botulinum toxin field also comprises therapeutic uses, basic research studies and biodefense issues. This review presents currently available detection methods, and new methods offering the potential of enhanced precision and reproducibility. While the immunological methods offer a range of benefits, such as rapid analysis time, reproducibility and high sensitivity, their implementation is subject to the availability of suitable tools and reagents, such as specific antibodies. Currently, the mass spectrometry approach is the most sensitive in vitro method for a rapid detection of active or inactive forms of BoNTs. However, these methods require inter-laboratory validation before they can be more widely implemented in reference laboratories. In addition, these surrogate in vitro models also require full validation before they can be used as replacement bioassays of potency. Cell-based assays using neuronal cells in culture recapitulate all functional steps of toxin activity, but are still at various stages of development; they are not yet sufficiently robust, due to high batch-to-batch cell variability. Cell-based assays have a strong potential to replace the mouse bioassay (MBA) in terms of BoNT potency determination in pharmaceutical formulations; they can also help to identify suitable inhibitors while reducing the number of animals used. However, the development of safe countermeasures still requires the use of in vivo studies to complement in vitro immunological or cell-based approaches.

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Differentiation of Gelsemium elegans-Containing Toxic Honeys and Non-Toxic Honeys by Near Infrared Spectroscopy Combined with Aquaphotomics

Huang,

Liu,

et al. 2023

Food Anal. Methods

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Development of an alternative approach for detecting botulinum neurotoxin type A in honey: Analysis of non-toxic peptides with a reference labelled protein via liquid chromatography-tandem mass spectrometry

Cited by 4 publications

References 41 publications

Current Trends and Technological Advancements in the Study of Honey Bee-Derived Peptides with an Emphasis on State-of-the-Art Approaches: A Review

Current Trends and Technological Advancements in the Study of Honey Bee-Derived Peptides with an Emphasis on State-of-the-Art Approaches: A Review

Recent Developments in Botulinum Neurotoxins Detection

Differentiation of Gelsemium elegans-Containing Toxic Honeys and Non-Toxic Honeys by Near Infrared Spectroscopy Combined with Aquaphotomics

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