Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection

Jaszczak, Ewa; Ruman, Marek; Narkowicz, Sylwia; Namieśnik, Jacek; Polkowska, Żaneta

doi:10.1155/2017/7157953

Cited by 21 publications

(6 citation statements)

References 18 publications

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“…Sim et al ( 2021) [21] and Jaszczak et al ( 2017) [23] applied this method to blood, urine, saliva, and sweat. In our study, the IC-PAD technique was also validated and applied to organs and gastric content, in addition to blood matrix.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of lethal cyanide poisoning. Analysis by Anion‐Exchange Chromatography with Pulsed Amperometric Detection

Zuccarello

Carnazza

Raffino

et al. 2022

Journal of Forensic Sciences

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Cyanide is a poison widely used in cases of suicide or homicide. Although various methods to identify and quantify this substance are reported in the literature, they are mainly validated on biological fluids (e.g., blood and urine). In the present study, the Anion-Exchange Liquid Chromatography with Pulsed Amperometric Detection (IC-PAD) method was validated on blood and, for the first time, on gastric content, and organs (brain, lung, and liver).

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Section: Discussionmentioning

confidence: 99%

Diagnosis of lethal cyanide poisoning. Analysis by Anion‐Exchange Chromatography with Pulsed Amperometric Detection

Zuccarello

Carnazza

Raffino

et al. 2022

Journal of Forensic Sciences

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“…The good repeatability of the method is indicated by the CV, which was less than 3%. Possible interferences such as sulfides and nitrates were eliminated by using dedicated SPE columns [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Ion Chromatography with Pulsed Amperometric Detection for Determining Cyanide in Urine and Meconium Samples

et al. 2021

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The parents’ addictions and eating habits have a significant influence on the child’s growth. The first stool of a newborn baby provides a large amount of information about xenobiotics transmitted by the mother’s body. The analytical technique used in the study is ion chromatography with pulsed amperometric detection (IC-PAD). The biological samples, which were obtained from women staying in a maternity ward and their partners, revealed cyanide concentrations in urine samples spanning 1.30–25.3 μg L−1. Meanwhile, the results of the meconium samples were in the range of 1.54 μg L−1 to 24.9 μg L−1. Under the optimized chromatographic conditions, the IC-PAD system exhibited satisfactory repeatability (R < 3%, n = 3) and good linearity in the range of 1–100 μg L−1. Thus, it proved to be an effective tool for monitoring trace cyanide concentration in a series of human body fluid matrices, including meconium. Based on the literature review, this is the first application of the IC-PAD analytical technique for the determination of cyanide ions in meconium samples.

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“…These working reference materials were prepared in Bhabha Atomic Research Centre, India, via nine interlaboratory comparison experiments. The cation exchange cartridge used for matrix separation is of Dionex make (OnGuard II H) and contains styrene-based strong acid resin in the H + form. , …”

Section: Experimental Sectionmentioning

confidence: 99%

Decay-Associated Fluorescence for Boron Determination in Uranium-Based Nuclear Fuels

Verma

2023

ACS Omega

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Herein is reported a novel as well as simple, sensitive, and cost-effective method for determination of boron by time-resolved fluorescence spectrometry in uranium-based nuclear fuels. Boron is complexed with fluorescent ligand chromotropic acid, and the complex formed is a measure of boron. Since the steady-state fluorescence spectra of excess ligand and complex are overlapping, the developed method emphasizes the power of time resolution. The signatory fluorescence decay times of ligand and complex are employed to derive their decay-associated spectra (DAS) and, thereby, spectroscopically eliminate the high background of ligand fluorescence. The calibration plot has a wide linear dynamic range of 5–100 ppb with r 2 better than 0.998. Precision is better than 5% at the 10 ppb level and 4% at the 50 ppb level ( n = 9). The detection limit is 1.5 ppb, and recovery of spiked boron (25 ppb) from the uranium samples was better than 94%. The developed method was validated by analyzing U 3 O 8 -based ILCE Standards and applied to enriched uranium fuel samples. The main advantage of the developed method is a reduction in sample size requirement due to better sensitivity and selectivity. This in turn reduces the load of uranium recovery from analytical waste, especially in the case of enriched uranium samples. Additionally, it eliminates the need of organic solvents/medium.

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Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection

Cited by 21 publications

References 18 publications

Diagnosis of lethal cyanide poisoning. Analysis by Anion‐Exchange Chromatography with Pulsed Amperometric Detection

Diagnosis of lethal cyanide poisoning. Analysis by Anion‐Exchange Chromatography with Pulsed Amperometric Detection

Ion Chromatography with Pulsed Amperometric Detection for Determining Cyanide in Urine and Meconium Samples

Decay-Associated Fluorescence for Boron Determination in Uranium-Based Nuclear Fuels

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