Development of an androgen‐deprivation induced and androgen suppressed human prostate cancer cell line

Abstract

We have developed an androgen-deprivation induced and androgen suppressed human prostate cancer cell line. This cell line is a valuable tool to study molecular mechanisms of the progression of androgen refractory prostate cancer and intermittent androgen suppression therapy.

Interleukin‐4 stimulates androgen‐independent growth in LNCaP human prostate cancer cells

“…IL-4 was expressed as a secretion rate into the culture medium in the units of pg/ml/24 hr/10 6 cells. The levels of PSA in the culture medium and serum of the tumor-bearing mouse were determined by ELISA as described previously [21,22].…”

“…The cell growth is reduced when LNCaP cells are cultured in androgen-deprived condition in the charcoal-stripped FBS compared to normal FBS [21]. To examine whether IL-4 enhances LNCaP cell growth in the charcoalstripped FBS condition (CS-FBC), parental LNCaP, neo control, and IL-4 overexpressing clones were cultured in media containing either FBS or CS-FBC, and the cell growth was determined.…”

“…LNCaP cells respond to androgen in a biphasic manner with a maximum stimulation at 1 nM of DHT and subsequent decrease at 10 nM of DHT [21]. To examine whether IL-4 expression affect the sensitivity of LNCaP cells in response to DHT treatment, IL-4 expressing LNCaP cells (C4A and C6A), parental LNCaP, and neo control cells were cultured in media containing charcoal-stripped FBS in the presence of different concentrations of DHT (from 0 to 10 nM).…”

Pim1 Regulates Androgen-Dependent Survival Signaling in Prostate Cancer Cells

“…IL-6 is known to phosphorylate STAT3 at tyrosine-705 and activate Pim1 [15,16] . LNCaP cells grown under androgen-deprived conditions for more than 1 year showed androgen-independent growth and upregulation of STAT3 [21] . Controversy on the role of STAT3 in PC3 cells has been increasing.…”

Androgen Action in Prostate Cancer

“…LNCaP cells grown in progressively androgen-depleted media selects for ''androgen-independent'' variants. But androgen response may be even more complicated; although xenografted cells grow equally well in castrate or intact hosts, they decrease growth rate approximately twofold upon androgen stimulation (Lee et al 2007). Other ''androgen-independent'' cells, such as CWR-R1 and LNCaP-C4-2, increase growth twofold when androgens are added to the media (Gregory et al 2001).…”