Development of an Anti-infective Coating on the Surface of Intraosseous Implants Responsive to Enzymes and Bacteria

Abstract

BackgroundBacterial proliferation on the endosseous implants surface presents a new threat to the using of the bone implants. Unfortunately, there is no effective constructed antibacterial coating which is bacterial anti-adhesion substrate-independent or have long-term biofilm inhibition functions. MethodsDrug release effect was tested in CMS solution and S. aureus. We used bacterial inhibition rate assays and protein leakageexperiment to analyze the in vitro antibacterial effect of (MMT/PLL-CHX)10 multilayer film. We used the CCK-8 assay to analyze the effect of (MMT/PLL-CHX)10 multilayer films on the growth and proliferation of rat osteoblasts. Rat orthopaedic implant-related infections model was constructed to test the antimicrobial activity effect of (MMT/PLL-CHX)10 multilayer films in vivo.ResultsIn this study, the (MMT/PLL-CHX)10 multilayer films structure were progressively degraded and showed well concentration-dependent degradation characteristics following incubation with Staphylococcus aureus and CMS solution. Bacterial inhibition rate assays and protein leakageexperiment showed high levels of bactericidal activity. While the CCK-8 analysis proved that the (MMT/PLL-CHX)10 multilayer films possess perfect biocompatibility. It is somewhat encouraging that in the in vivo antibacterial tests, the K-wires coated with (MMT/PLL-CHX)10 multilayer films showed lower infections incidence and inflammation than the unmodified group, and all parameters are close to SHAM group. Conclusion(MMT/PLL-CHX)10 multilayer films provides a potential therapeutic method for orthopaedic implant-related infections.

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“…In the development of anti-infective coatings on the surface of intraosseous implants that react to enzymes and bacteria, multilayer lms were prepared by traditional layer-by-layer self-assembly technology, and the thickness and frequency of materials deposited in each layer were obtained by QCM. It is concluded that with the increase of deposition steps, the frequency decreases and the dissipation increases steadily [32].After exploring the assembly behavior and mechanism of gene-loaded lipopolysaccharide amine nano-vesicles and hyaluronic acid polyelectrolyte multilayers by QCM, WeiTeng et al [33]found that the thickness of polyelectrolyte lms increases exponentially, and the law of the change of membrane quality and thickness is expounded. Because the frequency is inversely proportional to the mass, the frequency decreases, which indicates that the quality of the membrane increases.…”