Development of an antibacterial nanocomposite hydrogel for human dental pulp engineering

Bekhouche, Mourad; Bolon, Magali; Charriaud, Fanny; Lamrayah, Myriam; Costa, David da; Primard, Charlotte; Costantini, A.; Pasdeloup, Marielle; Gobert, Stéphanie; Malléin-Gerin, Frédéric; Verrier, Bernard; Ducret, Maxime; Farges, J.-C.

doi:10.1039/d0tb00989j

Cited by 37 publications

(31 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This approach to regenerative endodontics was shown to have good antibacterial effect against E. faecalis and promising biocompatibility properties since the synthesis of type I collagen was also observed when testing this hydrogel. [ 254 ] Similarly, excellent results in terms of antibacterial, angiogenic, and odontogenic effects were reported when bioactive glass nanospheres were used for the delivery of ions and growth factors. The release of copper ions and epidermal growth factor from gass nanospheres clearly demonstrated the efficacy of nanotherapy in suppressing the bacterial growth of E. faecalis and simulation of angiogenesis within endothelial cells.…”

Section: Applicationsmentioning

confidence: 99%

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

View full text Add to dashboard Cite

The oral cavity and oropharynx are complex environments that are susceptible to physical, chemical, and microbiological insults. They are also common sites for pathological and cancerous changes. The effectiveness of conventional locally-administered medications against diseases affecting these oral milieus may be compromised by constant salivary flow. For systemically-administered medications, drug resistance and adverse side-effects are issues that need to be resolved. New strategies for drug delivery have been investigated over the last decade to overcome these obstacles. Synthesis of nanoparticle-containing agents that promote healing represents a quantum leap in ensuring safe, efficient drug delivery to the affected tissues. Micro/nanoencapsulants with unique structures and properties function as more favorable drug-release platforms than conventional treatment approaches. The present review provides an overview of newly-developed nanocarriers and discusses their potential applications and limitations in various fields of dentistry and oral medicine.

show abstract

Section: Applicationsmentioning

confidence: 99%

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

View full text Add to dashboard Cite

show abstract

“… 112 For instance, fibrin hydrogel incorporating clindamycin-loaded Poly (D, L) Lactic Acid (PLA) nanoparticles can serve as an antibacterial and antibiofilm platform for the regeneration of devitalized dental pulp. 113 Alternatively, polyethylene glycol diacrylate (PEGDA)-based biomaterials can be used as injectable hydrogels in which to deliver DPSCs into the root canal lumen. 114 …”

Section: Biomaterials As a Platform For Delivery Of Dental Mscsmentioning

confidence: 99%

A narrative overview of utilizing biomaterials to recapitulate the salient regenerative features of dental-derived mesenchymal stem cells

Ansari

Sevari

2021

Int J Oral Sci

View full text Add to dashboard Cite

Tissue engineering approaches have emerged recently to circumvent many limitations associated with current clinical practices. This elegant approach utilizes a natural/synthetic biomaterial with optimized physiomechanical properties to serve as a vehicle for delivery of exogenous stem cells and bioactive factors or induce local recruitment of endogenous cells for in situ tissue regeneration. Inspired by the natural microenvironment, biomaterials could act as a biomimetic three-dimensional (3D) structure to help the cells establish their natural interactions. Such a strategy should not only employ a biocompatible biomaterial to induce new tissue formation but also benefit from an easily accessible and abundant source of stem cells with potent tissue regenerative potential. The human teeth and oral cavity harbor various populations of mesenchymal stem cells (MSCs) with self-renewing and multilineage differentiation capabilities. In the current review article, we seek to highlight recent progress and future opportunities in dental MSC-mediated therapeutic strategies for tissue regeneration using two possible approaches, cell transplantation and cell homing. Altogether, this paper develops a general picture of current innovative strategies to employ dental-derived MSCs combined with biomaterials and bioactive factors for regenerating the lost or defective tissues and offers information regarding the available scientific data and possible applications.

show abstract

“…In light of offering a biocompatible scenario for the disinfection of contaminated root canals, biodegradable drug delivery systems (e.g., scaffolds and hydrogels) laden with antimicrobial agents have gained the attention of researchers, and several studies are currently characterizing their clinical potential [11][12][13][14]. Concerning the use of fibrous scaffolds, electrospinning is a straightforward method for synthesizing antibiotic-laden polymeric nanofibers [6,9,15,16].…”

Section: Introductionmentioning

confidence: 99%

Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation

Ribeiro

Münchow

Bordini

et al. 2022

IJMS

View full text Add to dashboard Cite

This study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN- or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN- or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation.

show abstract

Development of an antibacterial nanocomposite hydrogel for human dental pulp engineering

Abstract: Hydrogel-based regenerative endodontic procedures (REPs) are considered to be very promising therapeutic strategies to reconstruct the dental pulp (DP) tissue in devitalized human teeth. However, the success of the regeneration...

Cited by 37 publications

References 56 publications

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

A narrative overview of utilizing biomaterials to recapitulate the salient regenerative features of dental-derived mesenchymal stem cells

Engineering of Injectable Antibiotic-laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation

Contact Info

Product

Resources

About