Development of an antibody-based colloidal gold immunochromatographic lateral flow strip test for natamycin in milk and yoghurt samples

Liu, Liqiang; Song, Shanshan; Zheng, Qinfen; Wu, Xiaoling; Kuang, Hua

doi:10.1080/09540105.2017.1337084

Cited by 13 publications

(6 citation statements)

References 31 publications

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“…Thus, these studies allowed us to select the most preferred reagents for the NAT-specific assay, which turned out to be more than an order of magnitude more sensitive than in previous reports [ 22 , 23 ] and in our recent group-specific immunoassay for polyenes [ 24 ]. The comparative analytical characteristics of the developed immunoassays from the available literature are given in Table 2 .…”

Section: Resultsmentioning

confidence: 97%

“…Comparative studies also allowed us to ensure that methanol was preferable to acetonitrile as an extractant. A number of reports regarding various analytical methods for the determination of NAT in various food matrices have also used methanol extraction [ 23 , 29 , 30 ]. These served as the bases for the choice of methanol as an extracting agent in the determination of NAT in the entire spectrum of the studied products.…”

Section: Resultsmentioning

confidence: 99%

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Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

2022

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The antifungal drug natamycin (NAT) is widely used in medicine and in the food industry as preservative E235 for a wide variety of foods. The risk of the development of resistance to NAT and its spread in relation to other polyene antibiotics is fraught with the emergence of incurable infections. This work is devoted to the development of an immunoassay to investigate the prevalence of NAT use for food preservation. Two immunogen designs based on tetanus toxoid, conjugated to NAT through different sites of hapten molecules, were compared in antibody generation. Assay formats using heterologous coating antigens were superior for both antibodies. The ELISA variant demonstrated the highest sensitivity (IC50 = 0.12 ng/mL), and a limit of detection of 0.02 ng/mL was selected for NAT determination. The optimized extraction procedure provided a recovery rate of 72–106% for various food matrixes with variations below 12%. Cyclodextrins, as well as NAT–cyclodextrin complex formulations, showed no interference with the quantification of NAT. One hundred and six food product brands, including baked goods, wines, beers, drinks, sauces, and yogurts, were tested to assess the prevalence of the undeclared use of NAT as a preservative. The screening examination revealed three positive yogurts with an undeclared NAT incorporation of 1.1–9.3 mg/kg.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

2022

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“…High-performance liquid chromatography (HPLC) coupled with either ultraviolet (UV) [3][4][5][6] or mass spectrometric (MS) [7][8][9][10] detectors are the most used techniques for the determination of NAT. Also, other analytical techniques were reported, such as voltammetry [11][12][13], and immunoassay [14], but to a lesser degree. These techniques either require costly equipment and/or well-trained analysts, lack high sensitivity, or utilize hazardous organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Rapid microwave synthesis of N and S dual-doped carbon quantum dots for natamycin determination based on fluorescence switch-off assay

Abdel-Hakim,

Belal,

Hammad

et al. 2023

Methods Appl. Fluoresc.

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A green, one-pot, quick, and easily synthesized nitrogen and sulfur co-doped carbon quantum dots (N,S-CDs) were obtained from cheap and readily available chemicals (sucrose, urea, and thiourea) using a microwave-assisted approach in about 4 min and utilized as a turn-off fluorescent sensor for estimation of natamycin (NAT). First, the effect of N and S doping on the microwave-synthesized CDs' quantum yield was carefully studied. CDs derived from sucrose alone failed to produce a high quantum yield; then, to increase the quantum yield, doping with heteroatoms was carried out using either urea or thiourea. A slight increase in quantum yield was observed upon using thiourea with sucrose, while an obvious enhancement of quantum yield was obtained when urea was used instead of thiourea. Surprisingly, using a combination of urea and thiourea together results in N,S-CDs with the highest quantum yield (53.5%), uniform and small particle size distribution, and extended stability. The fluorescent signal of N,S-CDs was quenched upon addition of NAT due to inner filter effect and static quenching in a manner that allowed for the quantitative determination of NAT over a range of 0.5-10.0 μg/ml (LOD=0.10 μg/ml). The N,S-CDs were applicable for determination of NAT in aqueous humor, eye drops, different environmental water samples, and bread with excellent performance. The selectivity study indicated excellent selectivity of the prepared N,S-CDs toward NAT with little interference from possibly interfering substances. In-silico toxicological evaluation of NAT was conducted to estimate its long-term toxicity and drug-drug interactions. Finally, the preparation of N,S-CDs, and analytical procedure compliance with the green chemistry principles was confirmed by two greenness assessment tools.

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“…Reducing or eliminating matrix interference of food samples has always been a serious problem that plagues researchers. Matrix interference of liquid food samples (such as water and milk) and semisolid food samples (such as honey, egg and yogurt) is easily to be eliminated by simple operations (such as dilution or centrifuge; Liu et al, 2017). However, for solid food samples (such as animal innards and muscle tissue), it is relatively difficult to eliminate complicated matrix interference (Chen et al, 2017; Zeng et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Development of a time‐resolved fluorescent immunochromatographic test for simultaneous detection of norfloxacin and sulfamethazine in pork samples through green pretreatment

Chen

Lin

2022

Journal of Food Safety

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A sensitive time‐resolved fluorescent microsphere immunochromatographic strip test (TRFM‐ICST) was established for the simultaneously rapid detection of norfloxacin (NOR) and sulfamethazine (SM2) in pork samples. Time‐resolved fluorescent europium (III) enclosed polystyrene microspheres conjugated with monoclonal antibody were used as probe to fulfill the analysis. Meanwhile, a green pretreatment system without organic solvent was developed for pork samples. Pork samples were processed by extracting buffer [citric acid buffer (0.01 M, pH 4.4) containing 2% (vol/vol) of Tween‐20 and 2% (wt/vol) of polyvinyl pyrrolidone (mass weight = 10,000)] and diluting buffer [phosphate buffered solution (0.01 M, pH 7.2) containing 1% (wt/vol) of Tween‐20 and 1% (wt/vol) of polyethylene glycol (mass weight = 400)]. The working range of TRFM‐ICST for NOR and SM2 in pork samples was 2.74–18.70 and 0.49–3.02 μg kg−1, respectively. Negative pork samples spiked with NOR and SM2 were detected by established TRFM‐ICST and high‐performance liquid chromatography (HPLC). The linear regression analysis yielded a good correlation between TRFM‐ICST and HPLC (Y = 0.9309X + 0.039, R2 = .9965, relative standard deviation (RSD) = 1.95%). The recovery of NOR and SM2 in pork samples was 94.1–114.4 and 93.0–102.0%, respectively, illustrating the proposed TRFM‐ICST can be applied for screening of high throughput pork samples.

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Development of an antibody-based colloidal gold immunochromatographic lateral flow strip test for natamycin in milk and yoghurt samples

Cited by 13 publications

References 31 publications

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

Rapid microwave synthesis of N and S dual-doped carbon quantum dots for natamycin determination based on fluorescence switch-off assay

Development of a time‐resolved fluorescent immunochromatographic test for simultaneous detection of norfloxacin and sulfamethazine in pork samples through green pretreatment

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