Development of an antigen-based rapid diagnostic test for the identification of blowfly (Calliphoridae) species of forensic significance

McDonagh, Laura; Thornton, Chris R.; Wallman, James F.; Stevens, Jamie R.

doi:10.1016/j.fsigen.2009.01.013

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…However, no reports have detected PMI-indicating proteins in post-mortem samples. Only one forensic entomology study has suggested identifying a blowfly species using LFA technology instead of a DNA-sequence-based method [41]. In the present study, we developed the LFA for a possible forensic application using the same antibodies used in the sandwich ELISA.…”

Section: Discussionmentioning

confidence: 99%

Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology

et al. 2016

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We investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of selected proteins were clearly divided into three groups: short-term, mid-term, and long-term PMI markers based on the half maximum intensity of intact protein expression. In kidney, glycogen synthase (GS) and glycogen synthase kinase-3β were degraded completely within 48 h making them short-term PMI markers. AMP-activated protein kinase α, caspase 3 and GS were short-term PMI markers in psoas muscle. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a mid-term PMI marker in both tissues. Expression levels of the typical long-term PMI markers, p53 and β-catenin, were constant for at least 96 h post-mortem in both tissues. The degradation patterns of GS and caspase-3 were verified by immunohistochemistry in both tissues. GAPDH was chosen as a test PMI protein to perform a lateral flow assay (LFA). The presence of recombinant GAPDH was clearly detected in LFA and quantified in a concentration-dependent manner. These results suggest that LFA might be used to estimate PMI at a crime scene.

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Section: Discussionmentioning

confidence: 99%

Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology

et al. 2016

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“…These peculiar features make blowflies the primary and most accurate indicators of the post mortem interval (Grassberger et al 2003). However, the immature stages pose a great identification challenge for forensic entomologists due to their lack of species-specific anatomical characters (McDonagh et al 2009). Identification of these stages using traditional methods (Mendonca et al 2008) and/ or advanced techniques (McDonagh et al 2009) is therefore the focus of many forensic studies.…”

Section: Introductionmentioning

confidence: 99%

“…However, the immature stages pose a great identification challenge for forensic entomologists due to their lack of species-specific anatomical characters (McDonagh et al 2009). Identification of these stages using traditional methods (Mendonca et al 2008) and/ or advanced techniques (McDonagh et al 2009) is therefore the focus of many forensic studies. The most popular method of identification in recent years includes molecular taxonomy (Harvey et al 2003; Smith and Baker 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, COI barcodes, or for that matter any single gene, seems unlikely to resolve the identities of all calliphorid species of forensic importance because such phylogenies only infer the evolutionary relationships for the particular gene used. Consequently, a switch to multigene approach becomes necessary (McDonagh et al 2009). To date only a handful studies have utilized such an approach (Wallman et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Zaidi

Wei

Shi

2011

Journal of Insect Science

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Contemporary studies in forensic entomology exhaustively evaluate gene sequences because these constitute the fastest and most accurate method of species identification. For this purpose single gene segments, cytochrome oxidase subunit I (COI) in particular, are commonly used. However, the limitation of such sequences in identification, especially of closely related species and populations, demand a multi-gene approach. But this raises the question of which group of genes can best fulfill the identification task? In this context the utility of five gene segments was explored among blowfly species from two distinct geographic regions, China and Pakistan. COI, cytochrome b (CYTB), NADH dehydrogenase 5 (ND5), nuclear internal transcribed spacers (ITS1 and ITS2), were sequenced for eight blowfly species including Chrysomya megacephala F. (Diptera: Calliphoidae), Ch. pinguis Walker, Lucilia sericata Meigen L. porphyrina Walker, L. illustris Meigen Hemipyrellia ligurriens Wiedemann, Aldrichina grahami Aldrich, and the housefly, Musca domestica L. (Muscidae), from Hangzhou, China; while COI, CYTB, and ITS2 were sequenced for four species, i.e. Ch. megacephala, Ch. rufifacies, L. cuprina, and the flesh fly, Sarcophaga albiceps Meigen (Sarcophagidae), from Dera Ismail Khan Pakistan. The results demonstrate a universal utility of these gene segments in the molecular identification of flies of forensic importance.

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“…One of its principle application is the determination of time since death or post-mortem interval (PMI), in suspicious death cases, either via insect species composition on the corpse or assessing the arthropods development rates [1,2]. Typically, at crime scene the immature stages are collected and used as biological clock to determine the minimum PMI [3]. Entomological evidence would be useful in assigning specimens to certain geographic locations [4].…”

Section: Introductionmentioning

confidence: 99%

Applications of forensic entomology: overview and update

Aly

Aldeyarbi

2020

amsik

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Streszczenie Mimo ogromnych postępów w badaniach podstawowych i stosowanych entomologia sądowa stale się rozwija i jest uznawana za interdyscyplinarną dziedzinę wiedzy. W zestawieniu z innymi naukami prawnymi i biologicznymi publikacje z zakresu entomologii sądowej są jednak stosunkowo skromne, podobnie jak liczba entomologów sądowych. Głównym celem niniejszej pracy jest przedstawienie znaczenia entomologii sądowej przez omówienie jej zastosowań, czynności podejmowane przez entomologów mogą bowiem dostarczać istotnych informacji dotyczących czasu, miejsca i przyczyny śmierci, ułatwiać identyfikację sprawców i/lub ofiar przestępstwa, a w niektórych przypadkach także określać stan zaniedbania ofiar przed śmiercią. W pracy omówiono też możliwość wykorzystywania zdobyczy najnowocześniejszych technologii. Interdyscyplinarność prac badawczych umożliwi dalsze zgłębianie tej dziedziny, przyczyniając się do wytyczania nowych obszarów badań z korzyścią dla rozwoju nauki oraz zastosowań kryminalistycznych.

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Development of an antigen-based rapid diagnostic test for the identification of blowfly (Calliphoridae) species of forensic significance

Cited by 13 publications

References 29 publications

Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology

Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology

Utility of Multi-Gene Loci for Forensic Species Diagnosis of Blowflies

Applications of forensic entomology: overview and update

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