The pomelo flavedo extract (PFE) from the pomelo peel (an agricultural waste) is well known with many functional properties. In this study, the incorporation of PFE into the bio-based polymer (pectin) for the formulation of the coating film was generally developed. The PFE showed a very high inhibitory effect against Pseudomonas aeruginosa (9.22 ± 0.37 mm of inhibition zone diameter), whereas Escherichia coli was found to be resistant to the PFE treatment. The incorporation of varying PFE contents into the pectin film with glycerol as a plasticizer for developing PFE-Gly-Pectin composite film and its effect on the film performance was investigated. PFE was found to reduce the swelling degree and increase the solubility of the Gly-Pectin film as well as increase the flexibility of the resulting film. However, the molecular structure, structural morphology, and contact angle of the Gly-Pectin film were insignificantly affected by the presence of PFE. The composite film at the PFE:Pectin ratio of 3.4 g/g was selected for the preliminary preservation of jackfruit bulbs which was stored at ambient temperature. As a result, coated jackfruit bulbs appeared to maintain a better quality after 3 days with an alleviated change in weight loss, color parameters, and total soluble solids, whereas the uncoated jackfruits showed signs of spoilage on Day 2 of the storage period. Therefore, the PFE conjugated pectin film suggested potential in the preservation of food materials.