Development of an artificial dermis preparation capable of silver sulfadiazine release

Kawai, Katsuya; Suzuki, Shigehiko; Tabata, Yasuhiko; Taira, Tsuguyoshi; Ikada, Yoshito; Nishimura, Yoshihiko

doi:10.1002/1097-4636(20011205)57:3<346::aid-jbm1177>3.0.co;2-8

Cited by 32 publications

(18 citation statements)

References 44 publications

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“…This cross-linking procedure is the same as that for preparation of artificial dermis reported previously. 22 Our previous study demonstrated that incorporation of PGA fiber (12 mg) (PGA:collagen weight mixing ratio of 5) enabled the collagen sponge to provide the best level of resistance to compression and in vitro cell proliferation. 23 On the basis of these results, collagen sponge incorporating 12 mg of PGA fiber was used in this study.…”

Section: Fabrication Of Collagen Sponge Incorporating Pga Fibermentioning

confidence: 99%

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

et al. 2005

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The objective of this study was to obtain fundamental knowledge about in vitro culture systems to enhance the proliferation and differentiation of mesenchymal stem cells (MSCs) in collagen sponge reinforced by the incorporation of poly(glycolic acid) (PGA) fiber. A collagen solution with PGA fiber homogeneously localized at PGA:collagen weight ratios of 0.67, 1.25, 2.5, and 5 was freezedried, followed by cross-linking of combined dehydrothermal, glutaraldehyde, and ultraviolet treatment. Scanning electron microscopy revealed that collagen sponges exhibited homogeneous and interconnected pore structures with an average size of 180 microm, irrespective of PGA fiber incorporation. When rat MSCs were seeded into collagen sponge with or without PGA fiber incorporation, more attached cells were observed in collagen sponge incorporating PGA fiber than in collagen sponge without PGA fiber incorporation, irrespective of the PGA:collagen ratio. The proliferation and osteogenic differentiation of MSCs in PGA-reinforced sponge at a weight ratio of 5 were greatly influenced by the culture method and growth conditions. Alkaline phosphatase (ALP) activity and osteocalcin content of MSCs cultured in PGA-reinforced sponge by the perfusion method became maximum at a flow rate of 0.2 mL/min, although they increased with culture time period. It may be concluded that appropriate perfusion conditions enable MSCs to positively improve the extent of proliferation and differentiation.

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Section: Fabrication Of Collagen Sponge Incorporating Pga Fibermentioning

confidence: 99%

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

et al. 2005

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“…Silver(I) sulfadiazine is one of the most popular silver based antimicrobial agents for treating infections on severe burn sites. It is a polymeric complex which releases silver ions slowly [20][21][22][23][24][25][26].…”

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confidence: 97%

Antimicrobial properties of highly fluorinated silver(I) tris(pyrazolyl)borates

Dias

Batdorf

Fianchini

et al. 2006

Journal of Inorganic Biochemistry

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“…Growth factor was loaded into the inner layer, which can accelerate chronic wound healing and prolong its stability [12,13]. The artificial dermis is impregnated with an antibiotic for application on contaminated wounds [14]. …”

Section: Discussionmentioning

confidence: 99%

The use of artificial dermis for corrective surgery on burn scars

Praditsuktavorn

Muangman

2015

Asian Biomedicine

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Background: Hypertrophic scarring from burn injuries on large skin areas is of great concern for both patients and attending physicians because of significant functional and cosmetic impairment. Surgery for scar corrections is challenging because of limitations on the availability of normal tissue. An alternative method for scar correction using artificial dermis is now possible. Artificial dermis can be used in the reconstruction of scars, burn injuries on large skin areas and in patients who do not have sufficient donor sites for skin grafts after scar excisions. Objective: To report the efficacy of artificial dermis on a 20 years old Thai man with severe scar contracture from burn injuries.Method: An open-label case study, the severe contracture case underwent scar excision and was applied with artificial dermis. Result: A 20-year-old man had severe scar contractures on his left arm, forearm, and elbow, and a shearing wound with a scar at his left elbow. Total scar excision was done on his left arm, forearm, elbow, and hand, and then followed by application of artificial dermis on to the wounds. After 2 weeks, neodermis formation was observed, after which an ultrathin split thickness skin graft was applied to the wounds. One month after sugery, the patient could extend his left elbow fully and the cosmetic result is satisfactory. Conclusion: Use of artificial dermis should be a new alternative modality in improving our strategy in correcting scar issues from burn injuries, especially on large burn scar areas with limited donor sites for skin grafts.

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Development of an artificial dermis preparation capable of silver sulfadiazine release

Cited by 32 publications

References 44 publications

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

Perfusion Culture Enhances Osteogenic Differentiation of Rat Mesenchymal Stem Cells in Collagen Sponge Reinforced with Poly(Glycolic Acid) Fiber

Antimicrobial properties of highly fluorinated silver(I) tris(pyrazolyl)borates

The use of artificial dermis for corrective surgery on burn scars

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