2023
DOI: 10.1186/s12985-022-01956-2
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Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro

Abstract: Background To investigate the mechanism of RNA silencing suppression, the genetic transformation of viral suppressors of RNA silencing (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes the VSR variations caused by different virus infections or limitations of host range. Moreover, identifying the insertion of the transgenic VSR gene is necessary to establish a model transgenic plant for the functional study of VSR. Methods … Show more

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Cited by 5 publications
(7 citation statements)
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“…For example, P0 proteins of viruses in the genera Polerovirus and Enamovirus may target and degrade AGO1 through the autophagy pathway ( Derrien et al., 2012 ; Fusaro et al., 2012 ; Michaeli et al., 2019 ; Barrios Barón et al., 2021 ), while PVX P25 destabilizes AGO1 via the 26S proteasome pathway ( Chiu et al., 2010 ). TuMV P1/HC-Pro degrades AGO1 through autophagy ( Hu et al., 2020 ; Hong et al., 2023 ), and TuMV VPg interacts with, and induces the degradation of SGS3 through both proteasome and autophagy pathways ( Cheng and Wang, 2017 ). Additionally, CMV-induced small peptide 1 inhibits antiviral RNA silencing defense by promoting autophagic degradation of SGS3 and RDR6 bodies ( Tong et al., 2021 ).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…For example, P0 proteins of viruses in the genera Polerovirus and Enamovirus may target and degrade AGO1 through the autophagy pathway ( Derrien et al., 2012 ; Fusaro et al., 2012 ; Michaeli et al., 2019 ; Barrios Barón et al., 2021 ), while PVX P25 destabilizes AGO1 via the 26S proteasome pathway ( Chiu et al., 2010 ). TuMV P1/HC-Pro degrades AGO1 through autophagy ( Hu et al., 2020 ; Hong et al., 2023 ), and TuMV VPg interacts with, and induces the degradation of SGS3 through both proteasome and autophagy pathways ( Cheng and Wang, 2017 ). Additionally, CMV-induced small peptide 1 inhibits antiviral RNA silencing defense by promoting autophagic degradation of SGS3 and RDR6 bodies ( Tong et al., 2021 ).…”
Section: Discussionmentioning
confidence: 99%
“…For instance, turnip mosaic virus (TuMV) employs its helper component-proteinase (HC-Pro) to bind physically with Arabidopsis thaliana Hua-Enhancer 1 (AtHEN1), a key component in the methylation of sRNAs, enabling it to destabilize vsiRNAs and allowing the virus to evade host defenses and persist within the plant ( Sanobar et al., 2021 ). In addition, TuMV HC-Pro can interact with AtAGO1 and promote its degradation through autophagy ( Wei et al., 2022 ; Hong et al., 2023 ). These findings highlight the intricate strategies plant viruses employ to circumvent host defense systems and establish successful infections.…”
Section: Introductionmentioning
confidence: 99%
“…The potyvirus fusion protein P1/HC-Pro was the first viral RNA silencing suppressor to be identified, with early studies on its mechanism showing that P1 enhances the suppressive effect of HC-Pro on RNA silencing [33,34]. Also known as RNA interference (RNAi), RNA silencing is a mechanism of innate immunity.…”
Section: Hc-pro Suppresses Rna Silencingmentioning
confidence: 99%
“…Interestingly, to enhance the suppressor activity of HC-Pro, P1 must be derived from the P1/HC-Pro precursor protein rather than simply ectopically expressed [135,136]. For this reason, studies of HC-Pro-induced RNA silencing suppression often involve a fused P1/HC-Pro protein that can be cleaved in vivo by the P1 protease to form two separate proteins, P1 and HC-Pro [136,137]. Since the RNA silencing suppression activity of the virus appears to be important in determining infectivity in potential hosts, the influence of P1 on HC-Pro may be important in the evolution of potyviruses [137].…”
Section: Order Patatavirales Family Potyviridaementioning
confidence: 99%
“…For this reason, studies of HC-Pro-induced RNA silencing suppression often involve a fused P1/HC-Pro protein that can be cleaved in vivo by the P1 protease to form two separate proteins, P1 and HC-Pro [136,137]. Since the RNA silencing suppression activity of the virus appears to be important in determining infectivity in potential hosts, the influence of P1 on HC-Pro may be important in the evolution of potyviruses [137]. Although the RNA silencing suppression activity of HC-Pro was discovered years ago, the exact molecular mechanisms have not been fully elucidated.…”
Section: Order Patatavirales Family Potyviridaementioning
confidence: 99%