Development of an automated liver perfusion system: The benefit of a hemofilter

Gullón, Lucía; Gutiérrez-Gutiérrez, Judit; Arco, Robert T Sánchez del; Rivera, Antonio; Fernández, Ignacio; Cañizo, Juan F. Del

doi:10.1177/0391398818783851

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…These additions aided preservation by filtering perfusate and maintaining physiologic acid-base balance. 92,93,117,118 Other studies have attempted to prevent substrate depletion by incorporating continuous infusions into perfusion circuits. Following the observation that isolated organs without intervention (controls) follow a pathologic sequence similar to the presentation of multi-organ failure following brain death (loss of vasomotor tone, diabetes insipidus with fluid loss and electrolyte disturbance, adrenal insufficiency), 119 investigators have supplemented perfusate with substrates that promote hemodynamic stability in brain dead donors (epinephrine, thyroxine, insulin, desmopressin, cortisol, fatty acids, and amino acids).…”

Section: Perfusatementioning

confidence: 99%

Prolonged (≥24 Hours) Normothermic (≥32 °C) Ex Vivo Organ Perfusion: Lessons From the Literature

et al. 2020

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For 2 centuries, researchers have studied ex vivo perfusion intending to preserve the physiologic function of isolated organs. If it were indeed possible to maintain ex vivo organ viability for days, transplantation could become an elective operation with clinicians methodically surveilling and reconditioning allografts before surgery. To this day, experimental reports of successfully prolonged (≥24 hours) organ perfusion are rare and have not translated into clinical practice. To identify the crucial factors necessary for successful perfusion, this review summarizes the history of prolonged normothermic ex vivo organ perfusion. By examining successful techniques and protocols used, this review outlines the essential elements of successful perfusion, limitations of current perfusion systems, and areas where further research in preservation science is required.

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Section: Perfusatementioning

confidence: 99%

Prolonged (≥24 Hours) Normothermic (≥32 °C) Ex Vivo Organ Perfusion: Lessons From the Literature

et al. 2020

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Recellularization of Decellularized Whole Organ Scaffolds: Elements, Progresses, and Challenges

Huang

Tan

et al. 2021

Decellularized Materials

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Evaluation of Blood Cell Destruction by Measuring Occlusion Distance

Kato,

Handa,

Yoshioka

et al. 2023

Wseas Transactions on Biology and Biomedicine

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Roller pumps are commonly used for electric motor-driven blood purification. Even the optimal occlusion for a roller pump is stimulated in JIS (Japanese Industrial Standard) -T1603, the blood cells can be destroyed if an applied pressure is too strong on the tube. On the other hand, the perfused blood volume might decrease if the pressure becomes weaker. Therefore, skilled operation is required. However, as there are no techniques to automatically measure occlusion, a highly reproducible method is urgently required to obtain an optimal setting. In this study, we classified the occlusion specified in JIS-T1603 into five categories (3, 6, 9, 12, and 15 drops/min) and measured those using a laser sensor. The distance between each occlusion was only a few microns. Based on the microscopic observation of the blood cell morphology at each occlusion, the blood cells with normal outlines were classified as normal blood cells, while those with protrusions were labeled as acanthocytes. Further, we calculated the normalized milligram index of hemolysis (mgNIH) to confirm hemolysis for each occlusion. By classifying occlusion into five categories and converting them into distances, we derived a safe, easy, and highly reproducible method.

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Development of an automated liver perfusion system: The benefit of a hemofilter

Cited by 3 publications

References 13 publications

Prolonged (≥24 Hours) Normothermic (≥32 °C) Ex Vivo Organ Perfusion: Lessons From the Literature

Prolonged (≥24 Hours) Normothermic (≥32 °C) Ex Vivo Organ Perfusion: Lessons From the Literature

Recellularization of Decellularized Whole Organ Scaffolds: Elements, Progresses, and Challenges

Evaluation of Blood Cell Destruction by Measuring Occlusion Distance

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