Development of an efficient mutagenesis technique using ion beams: Toward more controlled mutation breeding

Hase, Yoshihiro; Akita, Yusuke; Kitamura, Satoshi; Narumi, Issay; Tanaka, Atsushi

doi:10.5511/plantbiotechnology.12.0106a

Cited by 30 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For example, a pretreatment with sucrose, which upregulates the expression of anthocyanin biosynthesis genes, increases the number of flower-color mutants generated by carbon beam irradiation in petunia, although the frequency of chlorophyll mutations is almost unchanged [39]. Therefore, modulating the gene expression pattern may lead to increased controlled mutation, although the responsible mechanism will need to clarified [40].…”

Section: Effects Of Plant Tissue Type and Conditionmentioning

confidence: 99%

Frequency and Spectrum of Radiation-Induced Mutations Revealed by Whole-Genome Sequencing Analyses of Plants

Kim

2019

QuBS

View full text Add to dashboard Cite

Mutation breeding and functional genomics studies of mutant populations have made important contributions to plant research involving the application of radiation. The frequency and spectrum of induced mutations have long been regarded as the crucial determinants of the efficiency of the development and use of mutant populations. Systematic studies regarding the mutation frequency and spectrum, including genetic and genomic analyses, have recently resulted in considerable advances. These studies have consistently shown that the mutation frequency and spectrum are affected by diverse factors, including radiation type, linear energy transfer, and radiation dose, as well as the plant tissue type and condition. Moreover, the whole-genome sequencing of mutant individuals based on next-generation sequencing technologies has enabled the genome-wide quantification of mutation frequencies according to DNA mutation types as well as the elucidation of mutation mechanisms based on sequence characteristics. These studies will contribute to the development of a highly efficient and more controlled mutagenesis method relevant for the customized research of plants. We herein review the characteristics of radiation-induced mutations in plants, mainly focusing on recent whole-genome sequencing analyses as well as factors affecting the mutation frequency and spectrum.

show abstract

Section: Effects Of Plant Tissue Type and Conditionmentioning

confidence: 99%

Frequency and Spectrum of Radiation-Induced Mutations Revealed by Whole-Genome Sequencing Analyses of Plants

Kim

2019

QuBS

View full text Add to dashboard Cite

show abstract

“…The accumulated evidence suggests that ion beams efficiently induce mutations (Tanaka et al 2010;Hase et al 2012). Analyses of ionbeam irradiated mutants in higher plants showed that half of the mutants possessed large DNA alterations, whereas the rest had point-like mutations.…”

Section: Introductionmentioning

confidence: 98%

Tissue-dependent somaclonal mutation frequencies and spectra enhanced by ion beam irradiation in chrysanthemum

Okamura

Hase

Furusawa³

et al. 2014

Euphytica

Self Cite

View full text Add to dashboard Cite

We investigated the effect of tissue source selection in combination with ion beam irradiation on generating flower-color mutants in Chrysanthemum morifolium. Flower color mutation in the chrysanthemum cultivar YoMystery (purple flowers) was used as an index to estimate mutation frequency and the spectrum of mutations. Somaclones regenerated from petal and leaf tissues and ion-beam irradiated clones derived from the two tissues were analyzed for stem length and flower color mutation. Three Gy of argon ion beams was regarded as an appropriate dose for irradiation because the resulting color mutants maintained an adequate stem length suitable for commercial use even with high mutation frequencies. Statistical analysis of 7,258 individuals from three separate experiments revealed that (1) somaclones from petal tissue had a higher mutation frequency (2.91 %, r = 0.55) than leaf somaclones (2.01 %, r = 0.26);(2) the effect of tissue source on the frequency of flower color mutations was significantly enhanced when combined with ion-beam irradiation in that the mutation frequency of ion-beam irradiated petal clones was 6.47 % (r = 0.77), whereas that of ion-beam irradiated leaf clones was 3.89 % (r = 0.52); and (3) the spectrum of carotenoid color mutations was remarkably increased by ion-beam irradiation in that both leaf and petal ion-beam irradiated clones had brown, red and yellow flowers in contrast to the somaclones that only produced yellow flowers. These results suggest that intentional or directed induction of flower color mutants with practical value is possible by combining tissue source selection with ion beam irradiation.

show abstract

“…Therefore, gamma-ray irradiation has been the main treatment used in various plants, particularly ornamental plants such as orchids (Kikuchi, 2000), Anthurium (Puchooa, 2005), chrysanthemum (Kumar et al, 2012;Yamaguchi et al, 2008), and Paphiopedilum (Luan et al, 2012), for over 80 years. Recently, irradiation with ion beams, which are different from gamma-ray in that they are generated by accelerators and has specific mass and charge according to types of ions, has been proven to be very effective in mutation breeding in terms of biological effectiveness, mutation frequency, and range of mutation spectrum compared with gamma ray (Hase et al, 2012). New varieties in diverse crops including rice, verbena, carnation, and chrysanthemum have been developed using heavy-ion beams (Kanaya et al, 2008;Nagatomi, 2003;Okamura et al, 2003;Tanaka et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

DNA damage and oxidative stress induced by proton beam in Cymbidium hybrid

Lee

et al. 2015

Hortic. Environ. Biotechnol.

View full text Add to dashboard Cite

We investigated the radiation damages caused by two types of proton beam and gamma ray in the Cymbidium hybrid RB001 [(C. sinensis × C. goeringii) × Cymbidium spp.] to characterize proton beam as a new mutagen for Cymbidium mutation breeding. The protocorm-like bodies (PLBs) of Cymbidium hybrid were irradiated with a 45 MeV proton beam [mean linear energy transfer (LET) = 1.461 keV· m -1 ], a 100 MeV proton beam (LET = 0.7306 keV· m -1 ), and gamma ray (LET = 0.2 keV· m -1). The PLBs treated with radiation doses of 0-100 Gy were analyzed using the comet assay and their physiological responses as indices of radiation damage. In the comet assay, the 45 MeV proton beam caused significant damage to the DNA integrity, but the 100 MeV proton beam and gamma ray showed relatively little radiation damage between the untreated control and treated PLBs. Malondialdehyde (MDA) content, an index of the indirect effects of ionizing radiation, was increased slightly by the 45 MeV proton beam with higher-LET, but highly increased by the 100 MeV proton beam with lower-LET. These results suggested that the 100 MeV proton beam caused extreme oxidative stress. Therefore, proton beam have unique characteristics in DNA mutagenesis pattern according to its LETs. Based on these results, proton beam is expected to be a useful tool for developing new mutant varieties of Cymbidium.Additional key words: comet assay, gamma ray, linear energy transfer, physiological responses Hort. Environ. Biotechnol. 56(2):240-246. 2015.

show abstract

Development of an efficient mutagenesis technique using ion beams: Toward more controlled mutation breeding

Cited by 30 publications

References 22 publications

Frequency and Spectrum of Radiation-Induced Mutations Revealed by Whole-Genome Sequencing Analyses of Plants

Frequency and Spectrum of Radiation-Induced Mutations Revealed by Whole-Genome Sequencing Analyses of Plants

Tissue-dependent somaclonal mutation frequencies and spectra enhanced by ion beam irradiation in chrysanthemum

DNA damage and oxidative stress induced by proton beam in Cymbidium hybrid

Contact Info

Product

Resources

About