Development of an efficient virus-induced gene silencing method in petunia using the pepper phytoene desaturase (PDS) gene

Naing, Aung Htay; Song, He Sun; Lee, Je Min; Lim, Ki Byung; Kim, Chang Kil

doi:10.1007/s11240-019-01646-y

Cited by 9 publications

(2 citation statements)

References 32 publications

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“…Susceptibility to TRV infection can vary from plant to plant or from plant to plant of the same species at different stages of growth ( Deng et al., 2012 ; Naing et al., 2019 ). In this study, K.obovasta leaves exhibited a typical photobleaching phenotype, but leaves at different levels of development varied in their susceptibility to TRV.…”

Section: Discussionmentioning

confidence: 99%

Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata

Zhang,

Rao,

Chen

et al. 2023

Front. Plant Sci.

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As an important mangrove species, Kandelia obovata plays an irreplaceable role in the coastal ecosystem. However, due to a lack of genetic technology, there is limited research on its functional genes. As such, establishing an efficient and rapid functional verification system is particularly important. In this study,tobacco rattle virus (TRV) and the phytoene desaturase gene KoPDS were used as the vector and target gene, respectively, to establish a virus-induced gene silencing system (VIGS) in K. obovata. Besides, the system was also used to verify the role of a Chlorophyll a/b binding protein (Cab) gene KoCAB in leaf carbon sequestration of K. obovata.RNA-Seq and qRT-PCR showed that the highest gene-silencing efficiency could reach 90% after 10 days of inoculation and maintain above 80% after 15 days, which was achieved with resuspension buffer at pH 5.8 and Agrobacterium culture at OD600 of 0.4-0.6. Taken together, the TRV-mediated VIGS system established herein is the first genetic analysis tool for mangroves, which may greatly impel functional genomics studies in mangrove plants.

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Section: Discussionmentioning

confidence: 99%

Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata

Zhang,

Rao,

Chen

et al. 2023

Front. Plant Sci.

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“…Previously, Kim et al [15] reported that the silencing of the pepper PDS gene, which has high homology to the PDS gene in tomato and petunia plants, in pepper leaves and fruit led to distinct photo-bleaching. Recently, Naing et al [16] reported that the pepper PDS gene significantly suppressed endogenous PDS gene in petunia and reduced total chlorophyll content. Hence, pepper PDS was exploited to silence the endogenous PDS gene in tomato fruit.…”

Section: Introductionmentioning

confidence: 99%

Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes

et al. 2019

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Background Past research has shown that virus-induced phytoene desaturase (PDS) gene silencing via agroinjection in the attached and detached fruit of tomato plants results in a pale-yellow fruit phenotype. Although the PDS gene is often used as a marker for gene silencing in tomatoes, little is known about the role of PDS in fruit ripening. In this study, we investigated whether the pepper PDS gene silenced endogenous PDS genes in the fruit of two tomato cultivars, Dotaerang Plus and Legend Summer. Results We found that the pepper PDS gene successfully silenced endogenous PDS in tomato fruit at a silencing frequency of 100% for both cultivars. A pale-yellow silenced area was observed over virtually the entire surface of individual fruit due to the transcriptional reduction in phytoene desaturase (PDS), zeta-carotene (ZDS), prolycopene isomerase (CrtlSO), and beta-carotene hydroxylase (CrtR-b2), which are the carotenoid biosynthesis genes responsible for the red coloration in tomatoes. PDS silencing also affected the expression levels of the fruit-ripening genes Tomato AGAMOUS-LIKE1 (TAGL1), RIPENING INHIBITOR (RIN), pectin esterase gene (PE), lipoxygenase (LOX), FRUITFULL1/FRUITFUL2 (FUL1/FUL2), and the ethylene biosynthesis and response genes 1-aminocyclopropane-1-carboxylate oxidase 1 and 3 (ACO1 and ACO3) and ethylene-responsive genes (E4 and E8). Conclusion These results suggest that PDS is a positive regulator of ripening in tomato fruit, which must be considered when using it as a marker for virus-induced gene silencing (VIGS) experiments in order to avoid fruit-ripening side effects.

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Silencing of the Phytoene Desaturase (PDS) gene through virus infection using Tobacco Rattle Virus in Physalis grisea

Zhang,

Wang,

Liu

et al. 2024

Plant Cell Tiss Organ Cult

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Development of an efficient virus-induced gene silencing method in petunia using the pepper phytoene desaturase (PDS) gene

Cited by 9 publications

References 32 publications

Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata

Function verification of a chlorophyll a/b binding protein gene through a newly established tobacco rattle virus-induced gene silencing system in Kandelia obovata

Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes

Silencing of the Phytoene Desaturase (PDS) gene through virus infection using Tobacco Rattle Virus in Physalis grisea

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