Development of an electrochemical DNA biosensor for the detection of vitamin B12 (cyanocobalamin) at a carbon paste modified electrode with a manganese(II) complex

Dimitropoulou, Georgia; Karastogianni, Sophia; Girousi, Stella

doi:10.17145/jab.17.011

Cited by 10 publications

(2 citation statements)

References 37 publications

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“…The electrode was modified with an electrochemically produced polymer (Mn(thiophenyl-2-carboxylic acid) 2 (triethylonamine)), while cyanocobalamin was immobilized onto the modified electrode. The method proved to be simple, fast, selective, and with low detection limits (1210 µg/L) [ 187 ].…”

Section: Vitamin Analysis Using Electroanalytical Techniquesmentioning

confidence: 99%

Different Aspects of the Voltammetric Detection of Vitamins: A Review

Kiamiloglou,

Girousi

2023

Biosensors

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Vitamins comprise a group of organic chemical compounds that contribute significantly to the normal functioning of living organisms. Although they are biosynthesized in living organisms, some are also obtained from the diet to meet the needs of organisms, which is why they are characterized as essential chemical compounds. The lack, or low concentrations, of vitamins in the human body causes the development of metabolic dysfunctions, and for this reason their daily intake with food or as supplements, as well as the control of their levels, are necessary. The determination of vitamins is mainly accomplished by using analytical methods, such as chromatographic, spectroscopic, and spectrometric methods, while studies are carried out to develop new and faster methodologies and techniques for their analysis such as electroanalytical methods, the most common of which are voltammetry methods. In this work, a study is reported that was carried out on the determination of vitamins using both electroanalytical techniques, the common significant of which is the voltammetry technique that has been developed in recent years. Specifically, the present review presents a detailed bibliographic survey including, but not limited to, both electrode surfaces that have been modified with nanomaterials and serve as (bio)sensors as well as electrochemical detectors applied in the determination of vitamins.

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Section: Vitamin Analysis Using Electroanalytical Techniquesmentioning

confidence: 99%

Different Aspects of the Voltammetric Detection of Vitamins: A Review

Kiamiloglou,

Girousi

2023

Biosensors

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“…The detection was based on immobilization of a mononuclear Mn (II) complex film on the electrode surface (Mn-GROPE), by applying differential pulse voltammetry (DPV). The proposed modified electrode was previously synthesized and characterized from our group, and was used in the determination of vitamin B 12 with excellent analytical features [ 47 , 48 ]. This is the first time this electrode modifier was used in the detection of vitamin C, with also excellent analytical features, which are comparable and, in some cases, even better than those cited in the literature.…”

Section: Introductionmentioning

confidence: 99%

Selective Voltammetric Detection of Ascorbic Acid from Rosa Canina on a Modified Graphene Oxide Paste Electrode by a Manganese(II) Complex

2021

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Voltammetric techniques have been considered as an important analytical tool applied to the determination of trace concentrations of many biological molecules including ascorbic acid. In this paper, ascorbic acid was detected by square wave voltammetry, using graphene oxide paste as a working electrode, modified by a film of a manganese(II) complex compound. Various factors, such as the effect of pH, affecting the response characteristics of the modified electrode were investigated. The relationship between the peak height and ascorbic acid concentration within the modified working electrode was investigated, using the calibration graph. The equation of the calibration graph was found to be: I = 0.0550γac + 0.155 with R2 = 0.9998, where I is the SWV current and γac is the mass concentration of ascorbic acid. The LOD and LOQ of the proposed method were determined to be 1.288 μg/L and 3.903 μg/L, respectively. Several compounds, such as riboflavin, biotin, and ions, such as Fe and Cu, were tested and it seemed that they did not interfere with the analytic signal. The proposed procedure was successfully applied in the determination of ascorbic acid in Rosa canina hips.

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