Development of an electrochemical immunosensor based on gold nanoparticles incorporated chitosan biopolymer nanocomposite film for the detection of prostate cancer using PSA as biomarker

Suresh, Lakkavarapu; Brahman, Pradeep Kumar; Reddy, Kasarla Ramakrushna; Bondili, Jayakumar Singh

doi:10.1016/j.enzmictec.2017.10.009

Cited by 111 publications

(33 citation statements)

References 36 publications

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“…Incubation steps here were also consistent with conventional ELISA. Suresh et al modified the surface of a SPE using chitosan and gold nanoparticles (AuNPs) for the detection of PSA [ 141 ]. A sandwich immunoassay protocol was followed with HRP as the enzyme, but they amplified the signal using methylene blue as a redox mediator.…”

Section: Screen Printingmentioning

confidence: 99%

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Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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Section: Screen Printingmentioning

confidence: 99%

“…Electrochemical detection of PSA was monitored by cyclic voltammetry and square wave voltammetry. A dynamic range of 1–18 ng mL −1 was observed with LOD 1 pg mL −1 [ 141 ].…”

Section: Screen Printingmentioning

confidence: 99%

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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“…The food biosensor was still stable by the 20 th day retaining 96.3% of the initial current signal. The stability of the biosensor may be attributed to the utilization of CS and ABA [36,37]. To investigate the selectivity of the designed food biosensor, different food allergen proteins was tested (BSA, lysozyme, and casein).…”

Section: Reproducibility Stability and Selectivitymentioning

confidence: 99%

Fabrication of label-free electrochemical food biosensor for the sensitive detection of ovalbumin on nanocomposite-modified graphene electrode

2019

Biointerface Res Appl Chem

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In this work a highly sensitive label-free electrochemical food biosensor for the detection of egg allergen ovalbumin (Ova) was developed using nanocomposite consisting of iron oxide (Fe3O4) and palladium (Pd) nanoparticles with conducting polymer chitosan (CS). Pd nanoparticles were synthesized and seeded onto Fe3O4 nanoparticles, and dispersed in CS to form the nanocomposite. The nanocomposite (Fe3O4-Pd/CS) was drop-casted onto a screen printed graphene electrode (SPGE). Subsequently, 4-ABA was electrografted onto the modified SPGE/Fe3O4-Pd/CS. Carbodiimide chemistry was performed for the covalent conjugation of Ova-antibody (Ova-Ab) onto the electrode surface. The bimetallic nanocomposite portrayed excellent electrocatalytic effect due to the synergistic conductive effect of the Fe3O4-Pd nanomaterials and CS polymers and facilitated the electron movement between electrode surface and redox buffer. Additionally, ABA also enhanced electronic current on the surface of the electrode. Featuring high electron conductivity, the developed food biosensor demonstrated a large linear range to detect the Ova between 0.01 pg/mL – 1 µg/mL with a low limit of detection of 0.01 pg/mL. Furthermore, the proposed food biosensor portrayed very good selectivity with a high potential to detect Ova in real food sample.

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“…The immunosensor resulted in a highly selective and stable model, having a low PSA detection limit of 0.24 fg/mL [47]. Suresh et al (2018) applied an identical method, however, gold nanoparticles that were coated with a chitosan polymer were used to establish binding to the anti-PSA antibodies [48]. On the other hand, Vural et al (2018) proposed a method for PSA detection in blood serum samples that involved self-assembled AuNPs with peptide nanotube (PNT) and polyaniline (PANI) composites (PANI/AuNP–PNT) that were used to modify a pencil graphite electrode (PGE).…”

Section: Arena Of Gold Nanoparticles In Pcamentioning

confidence: 99%

Jumping on the Bandwagon: A Review on the Versatile Applications of Gold Nanostructures in Prostate Cancer

Sarkis

Ghanem

Rahme

2019

IJMS

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Prostate cancer (PCa) has remarkably emerged as a prominent disease in the face of the male population. Conventional treatments like prostatectomy or radiation can be curative only if PCa is diagnosed at an early stage. In the field of targeted therapy, a bevy of novel therapeutic approaches have left a landmark in PCa treatment and have proven to extend survival via distinct modes of actions. Nanotherapy has started to take root and has become the hype of the century by virtue of its abundant advantages. Scientists have invested a great deal of interest in the development of nanostructures such as gold nanoparticles (AuNPs), which hold particularly great hope for PCa theranostics. In this article, we present an overview of the studies published after 1998 that involve the use of different functionalized AuNPs to treat and diagnose PCa. Special reference is given to various in vitro and in vivo methods employed to shuttle AuNPs to PCa cells. Major studies show an enhancement of either detection or treatment of PCa when compared to their non-targeted counterparts, especially when AuNPs are tagged with specific ligands, such as antibodies, tea natural extracts, folate, anisamide, receptor inhibitors, and chitosan. Future approaches of treatment are dependent on those worthy multifunctional molecules, and are dictated by their ability to achieve a more versatile cancer therapeutic approach.

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Development of an electrochemical immunosensor based on gold nanoparticles incorporated chitosan biopolymer nanocomposite film for the detection of prostate cancer using PSA as biomarker

Cited by 111 publications

References 36 publications

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

Fabrication of label-free electrochemical food biosensor for the sensitive detection of ovalbumin on nanocomposite-modified graphene electrode

Jumping on the Bandwagon: A Review on the Versatile Applications of Gold Nanostructures in Prostate Cancer

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