Development of an electrospray approach to deposit complex molecules on plasma modified surfaces

Kitching, K.J.; Lee, H. N.; Elam, W. T.; Johnston, E. E.; MacGregor, H.; Miller, R. J. Dwayne; Tureček, František; Ratner, Buddy D.

doi:10.1063/1.1618013

Cited by 28 publications

(63 citation statements)

References 26 publications

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“…In principle, reactive landing could be done on the MALDI plate directly, without having to mount externally prepared surfaces. Reasons for our current approach include the higher price of commercial MALDI plates and the fact that smaller chips of cut stainless steel are easier to handle inside the soft-landing instrument, which has relatively narrow chambers [30].…”

Section: Alteration Of Standard Maldi Platesmentioning

confidence: 99%

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Reactive landing of gas-phase ions as a tool for the fabrication of metal oxide surfaces for in situ phosphopeptide enrichment

Blacken

Volný

Diener

et al. 2009

J. Am. Soc. Mass Spectrom.

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Zirconium, titanium, and hafnium oxide-coated stainless steel surfaces are fabricated by reactive landing of gas-phase ions produced by electrospray ionization of group IVB metal alkoxides. The surfaces are used for in situ enrichment of phosphopeptides before analysis by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. To evaluate this method we characterized ZrO 2 (zirconia) surfaces by (1) comparison with the other group IVB metal oxides of TiO 2 (titania) and HfO 2 (hafnia), (2) morphological characterization by SEM image analysis, and (3) dependence of phosphopeptide enrichment on the metal oxide layer thickness. Furthermore, we evaluated the necessity of the reactive landing process for the construction of useful metal oxide surfaces by preparing surfaces by electrospray deposition of Zr, Ti, and Hf alkoxides directly onto polished metal surfaces at atmospheric pressure. Although all three metal oxide surfaces evaluated were capable of phosphopeptide enrichment from complex peptide mixtures, zirconia performed better than hafnia or titania as a result of morphological characteristics illustrated by the SEM analysis. Metal oxide coatings that were fabricated by atmospheric pressure deposition were still capable of in situ phosphopeptide enrichment, although with inferior efficiency and surface durability. We show that zirconia surfaces prepared by reactive landing of gas-phase ions can be a useful tool for high throughput screening of novel phosphorylation sites and quantitation of phosphorylation kinetics. (J Am Soc Mass Spectrom 2009, 20, 915-926)

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Section: Alteration Of Standard Maldi Platesmentioning

confidence: 99%

“…It is an integral part of the soft and reactive landing instrument as described previously [30]. The surface treatment was carried out at 60-W radio-frequency power for 10 min in 250 mTorr of flowing oxygen gas.…”

Section: In Situ Plasma Treatmentmentioning

confidence: 99%

Reactive landing of gas-phase ions as a tool for the fabrication of metal oxide surfaces for in situ phosphopeptide enrichment

Blacken

Volný

Diener

et al. 2009

J. Am. Soc. Mass Spectrom.

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“…The liquid is fed through a hollow capillary and subjected to an electric field by applying a potential difference between the capillary and a counter electrode. Electrospray has been used for the atomization and deposition of a wide range of materials including DNA, 26 biocompatible polymers, 27,28 polysaccharides, and a range of proteins [29][30][31][32][33][34][35] with retention of structure and function. For example alkaline phosphatase was electrospray deposited 31 onto a conductive quartz electrode.…”

Section: Introductionmentioning

confidence: 99%

Electrospray deposited fibronectin retains the ability to promote cell adhesion

et al. 2010

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Scaffolds for tissue engineering require the correct biochemical cues if the seeded cells are to migrate into the scaffold and proliferate. For complex tissues this would require precise patterning of the scaffold structure with the particular biochemical cue required at each location on the scaffold. Electrospray enables the deposition of a wide number of biomolecules onto surfaces and can be used for precise patterning. We assessed the functionality of a key cell-adhesion molecule, fibronectin, after depositing it onto a surface using the electrospray technique. The addition of polypropylene glycol allowed a stable spray to be obtained from solutions with a range of fibronectin concentrations. Immunoassay tests showed that the amount of fibronectin retained on the surface was proportional to that sprayed from the solution. Increasing the surface density of fibronectin deposited onto silicon surfaces enhanced fibroblast attachment. The fibronectin thus appears to have retained its cell attachment functionality after undergoing the electrospray process. Since recent advances allow electrospray to pattern material from solution with micrometre accuracy this may allow materials to be biologically functionalized on a similar scale.

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“…Simple organic cations [7,8], metal clusters [9], polysaccharides [10], a nucleotide [11], and intact viruses [12][13][14] have all been the targets of examination by ion soft-landing. The separation, purification, and storage of chemical species by a completely non-destructive, mass spectrometric approach are the objectives of the studies currently underway in our laboratory.…”

mentioning

confidence: 99%

Ion soft-landing into liquids: Protein identification, separation, and purification with retention of biological activity

Gologan

Takáts

Alvarez

et al. 2004

J. Am. Soc. Mass Spectrom.

122

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Protein ions, after mass spectrometric separation, can be soft-landed into liquid surfaces with preservation of their native structures. Retention of biological activity is strongly favored in glycerol-based surfaces but not in self-assembled monolayer solid surfaces. Soft-landing efficiency for multiply-charged hexokinase ions was found to be some four times higher for a glycerol/fructose liquid surface than for a fluorinated self-assembled monolayer surface. Soft-landing into liquid surfaces is also shown to allow (1) protein purification, (2) on-surface identification of the soft-landed material using MALDI, and (3) protein identification by in-surface tryptic digestion. Pure lysozyme was successfully isolated from different mixtures including an oxidized, partially decomposed batch of the protein and a partial tryptic digest. Liquid glycerol/carbohydrate mixtures could be used directly to record MALDI spectra on the soft-landed compounds provided they were fortified in advance with traditional MALDI matrices such as p-nitroaniline and ␣-cyano-4-hydroxycinnamic acid. Various proteins were soft-landed and detected on-target using these types of liquid surface. Soft-landing of multiply-charged lysozyme ions onto fluorinated self-assembled monolayer surfaces was found to occur with a limited amount of neutralization, and trapped multiply-charged ions could be desorbed from the surface by laser desorption. Initial data is shown for a new approach to protein identification that combines top-down and bottom-up approaches by utilizing protein ion soft-landing from a protein mixture, followed by tryptic digestion of the landed material and detection of characteristic tryptic fragments by MALDI. , has been applied in experiments ranging from studies using chemically inert and structurally organized self-assembled monolayers (SAMs) as substrates for ion storage [2], to studies of ion mobility through ice and vapor deposited films [3,4], to experiments aimed at developing a separation method in a form of preparatory mass spectrometry [5,6]. Simple organic cations [7,8] [12][13][14] have all been the targets of examination by ion soft-landing. The separation, purification, and storage of chemical species by a completely non-destructive, mass spectrometric approach are the objectives of the studies currently underway in our laboratory.In a preliminary report [5], we described a method which showed the potential for generating biologically active protein chips from protein mixtures by massselective ion soft-landing. After ionization of the mixture by electrospray (ESI), individual protein ions were mass-selected and soft-landed at predetermined locations on the surface. The results showed that the use of mass spectrometry as a separation method in biology provides high selectivity because components with different molecular formulas can be separated and softlanded. However, retention of the biological activity of a protein or other biomolecule in the course of this (or any mass spectrometry experiment) is a much more chall...

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Development of an electrospray approach to deposit complex molecules on plasma modified surfaces

Cited by 28 publications

References 26 publications

Reactive landing of gas-phase ions as a tool for the fabrication of metal oxide surfaces for in situ phosphopeptide enrichment

Reactive landing of gas-phase ions as a tool for the fabrication of metal oxide surfaces for in situ phosphopeptide enrichment

Electrospray deposited fibronectin retains the ability to promote cell adhesion

Ion soft-landing into liquids: Protein identification, separation, and purification with retention of biological activity

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