Development of an Elisa test to differentiate vaccinated from pseudorabies infected animals

Abstract

potential applications in the field of diagnosis and vaccination. The use of monoclonal antibodies in research and survey laboratories is now widespread concerning viruses of the classical and African swine fever, Aujeszky's disease, Transmissible gastroenteritis and is extending to bacteriology (Salmonella, Mycoplasma). Nucleic acid probes, presently used as research tools, might also be applied to routine detection of infectious agents. Genetic engineering has allowed the development of several new strategies for immunoprophylaxis : synthetic peptides, recombinant antigens, non-pathogenic live vectors, attenuation by gene deletion. So far, the only commercialised recombinant vaccine is against E. coli-enterotoxicosis in the piglet. Designing of recombinant attenuated strains of pseudorabies virus is in rapid progress. The possibility to differentiate vaccinated from infected animals will be one of the major advantages of the future vaccines.

Protection of pigs against Aujeszky's disease by DNA vaccination.

“…Especially, gD has repeatedly been tested for efficacy in subunit or vectorexpressed form and shown to provide significant protection CBEE DNA immunization against Aujeszky's disease DNA immunization against Aujeszky's disease against the clinical manifestation of Aujeszky's disease (Eloit et al, 1988 ;Marchioli et al, 1987). MAbs against gD are most potent in neutralizing virus infectivity in the presence and absence of complement (Coe & Mengeling, 1990 ;Marchioli et al, 1988 ;Wathen & Wathen, 1984).…”

“…The most potent virus-neutralizing antibodies are directed against glycoproteins C and D (gC and gD) of PrV (Coe & Mengeling, 1990 ;Eloit et al, 1988 ;Hampl et al, 1984 ;Marchioli et al, 1988 ;Mukamoto et al, 1991 ;Wathen & Wathen, 1984), and a large part of the neutralizing activity in convalescent sera of swine appears to be directed against gC (Ben-Porat et al, 1986). gC has also been reported as a target for porcine cytotoxic T-cells (Zuckermann et al, 1990).…”