Development of an ELISA to detect antibodies to a protective lipopolysaccharide fraction of Leptospira borgpetersenii serovar hardjo in cattle

Yan, Keding; Ellis, William; Mackie, D.P.; Taylor, Mark J.; McDowell, S.W.J.; Montgomery, J.M.

doi:10.1016/s0378-1135(99)00103-0

Cited by 29 publications

(27 citation statements)

References 32 publications

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“…The Linnodee Leptospira ELISA Kit TM (Linnodee Animal Care, Ballyclare, Northern Ireland) [34,35] was used to test all serum samples. This ELISA detects an antibody response to a lipopolysaccharide outer envelope epitope common to both Leptospira borgpetersenii serovar Hardjo and Leptospira interrogans serovar Hardjo [35].…”

Section: Methodsmentioning

confidence: 99%

Seroprevalence of Leptospira Hardjo in the Irish suckler cattle population

et al. 2012

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BackgroundPrior to the present study, the seroprevalence of leptospirosis in Irish suckler herds was unknown. In this study, we describe the herd and animal-level prevalence of Leptospira Hardjo infection in the Irish suckler cattle population. For the purposes of the study, the 26 counties of the Republic of Ireland were divided into 6 regions from which a representative number of herds were selected. A herd was considered eligible for sampling if it was not vaccinating against leptospirosis and if it contained ≥ 9 breeding animals of beef breed ≥ 12 months of age. In total, 288 randomly selected herds were eligible for inclusion in the seroprevalence dataset analysis. Serological testing was carried out using a commercially available monoclonal antibody-capture ELISA, (sensitivity 100%; specificity 86.67%).ResultsHerds were categorised as either “Free from Infection” or “Infected” using the epidemiological software tool, FreeCalc 2.0. Using this classification, 237 herds were “Infected” (82.29%). The South West and South East regions had the highest herd prevalence. The regional effect on herd prevalence was largely mirrored by breeding herd size. A true animal-level prevalence of 41.75% was calculated using the epidemiological software tool, TruePrev. There was a statistically significant regional trend, with true prevalence being highest in the South East (P < 0.05). The median Breeding Herd Size (BHS), when categorised into quartiles, had a statistically significant influence on individual animal true seroprevalence (P < 0.001); true seroprevalence increased with increasing BHS.ConclusionsLeptospirosis is a widespread endemic disease in the Republic of Ireland. It is possible that economic losses due to leptospirosis in unvaccinated Irish suckler herds may be underestimated.

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Section: Methodsmentioning

confidence: 99%

Seroprevalence of Leptospira Hardjo in the Irish suckler cattle population

et al. 2012

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“…The Linnodee Leptospira ELISA Kit™ (Linnodee Animal Care, Ballyclare, Northern Ireland) [28-30] was used to test all serum samples [25]. This ELISA detects an IgG antibody response to a lipopolysaccharide outer envelope epitope common to both Leptospira borgpetersenii serovar Hardjo and Leptospira interrogans serovar Hardjo [30].…”

Section: Methodsmentioning

confidence: 99%

“…This ELISA detects an IgG antibody response to a lipopolysaccharide outer envelope epitope common to both Leptospira borgpetersenii serovar Hardjo and Leptospira interrogans serovar Hardjo [30]. Participating herds in the study had (i) to be unvaccinated, (ii) to contain ≥ 9 eligible breeding animals (bulls and breeding females ≥ 12 months of age) and (iii) to have breeding animals of beef breeds only.…”

Section: Methodsmentioning

confidence: 99%

Herd-level risk factors associated with Leptospira Hardjo seroprevalence in Beef/Suckler herds in the Republic of Ireland

et al. 2012

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BackgroundThe aim of the present study was to investigate risk factors for herd seropositivity to Leptospira Hardjo in Irish suckler herds. Herds were considered eligible for the study if they were unvaccinated and contained ≥ 9 breeding animals of beef breed which were ≥ 12 months of age. The country was divided into six regions using county boundaries. Herd and individual animal prevalence data were available from the results of a concurrent seroprevalence study. Herds were classified as either "Free from Infection" or "Infected" based on a minimum expected 40% within-herd prevalence.Questionnaires were posted to 320 farmers chosen randomly from 6 regions, encompassing 25 counties, of the Republic of Ireland. The questionnaire was designed to obtain information about vaccination; reproductive disease; breeding herd details; the presence of recognized risk factors from previous studies; and husbandry on each farm. Data collected from 128 eligible herds were subjected to statistical analysis.ResultsFollowing the use of Pearson's Chi-Square Test, those variables associated with a herd being "infected" with a significance level of P < 0.2 were considered as candidates for multivariable logistic regression modelling. Breeding herd size was found to be a statistically significant risk factor after multivariable logistic regression. The odds of a herd being positive for leptospiral infection were 5.47 times higher (P = 0.032) in herds with 14 to 23 breeding animals compared with herds with ≤ 13 breeding animals, adjusting for Region, and 7.08 times higher (P = 0.033) in herds with 32.6 to 142 breeding animals.ConclusionsBreeding herd size was identified as a significant risk factor for leptospiral infection in Irish suckler herds, which was similar to findings of previous studies of leptospirosis in dairy herds.

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“…In contrast to the applications of ELISA for diagnosis of human infection, in which broadly reactive assays are generally desirable and few serovar-specific assays have been developed (395), veterinary applications have been directed towards detection of serovar-specific antibodies, particularly for detection of infection in food animals. ELISA methods have described for detection of serovar pomona (134,573) and hardjo (5,58,573,653) infection in cattle and hardjo in sheep (9). Several assays are available commercially for serodiagnosis of bovine hardjo infection and have been evaluated (642).…”

Section: Downloaded Frommentioning

confidence: 99%

Leptospirosis

2001

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Leptospirosis is a worldwide zoonotic infection with a much greater incidence in tropical regions and has now been identified as one of the emerging infectious diseases. The epidemiology of leptospirosis has been modified by changes in animal husbandry, climate, and human behavior. Resurgent interest in leptospirosis has resulted from large outbreaks that have received significant publicity. The development of simpler, rapid assays for diagnosis has been based largely on the recognition that early initiation of antibiotic therapy is important in acute disease but also on the need for assays which can be used more widely. In this review, the complex taxonomy of leptospires, previously based on serology and recently modified by a genotypic classification, is discussed, and the clinical and epidemiological value of molecular diagnosis and typing is also evaluated

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Development of an ELISA to detect antibodies to a protective lipopolysaccharide fraction of Leptospira borgpetersenii serovar hardjo in cattle

Cited by 29 publications

References 32 publications

Seroprevalence of Leptospira Hardjo in the Irish suckler cattle population

Seroprevalence of Leptospira Hardjo in the Irish suckler cattle population

Herd-level risk factors associated with Leptospira Hardjo seroprevalence in Beef/Suckler herds in the Republic of Ireland

Leptospirosis

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