Development of an embryo transport and analysis system: Material biocompatablity

Chan, Nicole; Lyman, Joseph T.; Choi, S-J; Zeringue, Henry C.; Glasgow, Ian; Beebe, David J.; Wheeler, M. B.

doi:10.1016/s0093-691x(99)91793-4

Cited by 13 publications

(6 citation statements)

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“…2b. All the materials used had previously been tested for biocompatibility 16,17 and no adverse effect had been reported on the early culture of mouse embryos. The detailed procedures for fabrication the microchannels devices has been previously reported.…”

Section: Microchannel Device Fabricationmentioning

confidence: 99%

Embryonic development in the mouse is enhanced via microchannel culture

et al. 2004

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Microfluidic devices (microchannels) have been fabricated and tested for embryo culture. Three different microfabrication materials (silicon, polydimethylsiloxane (PDMS), and borosilicate) were used to fabricate the microchannels. The objective of this study was to determine if static microchannels permitted culture of mouse embryos to the blastocyst stage. Groups of 10 two-cell ICR x B6SJL/F1 mouse embryos were cultured for 96 hours in 4 different physical culture systems: 1) silicon/borosilicate microchannels, 2) PDMS/borosilicate microchannels, and 3) standard microdrops. Embryos cultured in the silicon/borosilicate and PDMS/borosilicate microchannels exhibited a faster rate of cleavage (P < 0.05), and produced more blastocysts (P < 0.01) than control microdrops. Furthermore, microchannels had a lower percentage of degenerated embryos than control embryos (P < 0.01). The results suggest that the microchannel culture systems may provide a culture environment that more closely mimics the in vivo environment.

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Section: Microchannel Device Fabricationmentioning

confidence: 99%

Embryonic development in the mouse is enhanced via microchannel culture

et al. 2004

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“…Previous studies have tested the biocompatibility of materials such as PS (standard petri dish material), polymides, silicons, and PDMS, assessing mice embryo development and pig sperm motility parameters. [38][39][40] However, to the authors' best knowledge, this is the first study that has tested the biocompatibility and absence of the toxicity of COC on bovine gametes. Table 2 shows the significantly higher percentages of sperm penetration observed in the four-well culture dish when compared with the microfluidic device at 18 h after IVF (61.44% vs. 35.63%, respectively; p < 0.05).…”

Section: Time-dependent On-chip Sperm Evaluationmentioning

confidence: 99%

Section: Time-dependent On-chip Sperm Evaluationmentioning

confidence: 99%

Rapid Prototyping of a Cyclic Olefin Copolymer Microfluidic Device for Automated Oocyte Culturing

Berenguel-Alonso

Sabés-Alsina

Morató

et al. 2017

SLAS TECHNOLOGY: Translating Life Sciences Innovation

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Assisted reproductive technology (ART) can benefit from the features of microfluidic technologies, such as the automation of time-consuming labor-intensive procedures, the possibility to mimic in vivo environments, and the miniaturization of the required equipment. To date, most of the proposed approaches are based on polydimethylsiloxane (PDMS) as platform substrate material due to its widespread use in academia, despite certain disadvantages, such as the elevated cost of mass production. Herein, we present a rapid fabrication process for a cyclic olefin copolymer (COC) monolithic microfluidic device combining hot embossing—using a low-temperature cofired ceramic (LTCC) master—and micromilling. The microfluidic device was suitable for trapping and maturation of bovine oocytes, which were further studied to determine their ability to be fertilized. Furthermore, another COC microfluidic device was fabricated to store sperm and assess its quality parameters over time. The study herein presented demonstrates a good biocompatibility of the COC when working with gametes, and it exhibits certain advantages, such as the nonabsorption of small molecules, gas impermeability, and low fabrication costs, all at the prototyping and mass production scale, thus taking a step further toward fully automated microfluidic devices in ART.

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“…As PDMS is cheap and possesses gas permeability and biocompatibility, most microfluidic devices related to biological research are made of PDMS [ 41 ]. Some studies, concerned with microfluidic application in the IVF field, were performed to evaluate the real biocompatibility of the materials required to build a device [ 42 , 43 , 44 , 45 ]; these initial studies were performed using mouse embryos [ 45 ] and pig oocytes [ 46 ]. The results showed that many materials such as silicon nitrate, silicon oxide, borosilicate glass, chromium, gold, titanium and polydimethylsiloxane (PDMS) have no negative impact on embryo development [ 42 , 45 , 46 , 47 ].…”

Section: Introductionmentioning

confidence: 99%

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

2021

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Infertility is a state of the male or female reproductive system that is defined as the failure to achieve pregnancy even after 12 or more months of regular unprotected sexual intercourse. Assisted reproductive technology (ART) plays a crucial role in addressing infertility. Various ART are now available for infertile couples. Fertilization in vitro (IVF), intracytoplasmic sperm injection (ICSI) and intrauterine insemination (IUI) are the most common techniques in this regard. Various microfluidic technologies can incorporate various ART procedures such as embryo and gamete (sperm and oocyte) analysis, sorting, manipulation, culture and monitoring. Hence, this review intends to summarize the current knowledge about the application of this approach towards cell biology to enhance ART.

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Development of an embryo transport and analysis system: Material biocompatablity

Cited by 13 publications

References 0 publications

Embryonic development in the mouse is enhanced via microchannel culture

Embryonic development in the mouse is enhanced via microchannel culture

Rapid Prototyping of a Cyclic Olefin Copolymer Microfluidic Device for Automated Oocyte Culturing

A Review on Microfluidics: An Aid to Assisted Reproductive Technology

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