Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells

Pécora, Andrea; Malacari, D.A.; Aguirreburualde, María Sol Pérez; Bellido, Demian; Escribano, José M.; Santos, María José Dus; Wigdorovitz, Andrés

doi:10.1016/j.ram.2014.12.004

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…In the present study, the prevalence of ETEC genes ( estA and elt ) was lower than 3% in each case, which was lower than previous findings in the region ( 47 ). Within the 303 isolates examined, 6 belonged to EPEC ( eae +) and only 2 to EHEC/STEC ( eae +/ stx1 +).…”

Section: Discussioncontrasting

confidence: 92%

Zoonotic Potential and Antibiotic Resistance of <i>Escherichia coli</i> in Neonatal Calves in Uruguay

et al. 2017

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Escherichia coli is one of the main etiological agents of neonatal calf diarrhea (NCD). The objective of this study was to assess the presence of virulence genes, genetic diversity, and antibiotic resistance mechanisms in E. coli associated with NCD in Uruguay. PCR was used to assess the presence of intimin, Shiga-like toxin, and stable and labile enterotoxin genes. Resistance to fluoroquinolones and oxyimino-cephalosporins was estimated on Müller-Hinton agar plates. Further antibiotic disc-diffusion tests were performed to assess bacterial multi-resistance. The presence of PMQR, ESBL, MCR-1, and integron genes was evaluated. Isolates were typed using ERIC-PCR, and 20 were selected for MLST, adhesion to Hep-2 cells, in vitro biofilm formation, and eukaryotic cytotoxicity. The prevalence of ETEC genes was lower than 3% in each case (estA and elt). Six isolates were EPEC (eae+) and 2 were EHEC/STEC (eae+/stx1+). The results of a diversity analysis showed high genetic heterogenicity among isolates. Additionally, different sequence types, including ST10, ST21, and ST69, were assigned to selected isolates. Thirty-six percent (96/264) of the isolates were fluoroquinolone-resistant, with 61/96 (63.5%) being multidrug-resistant. Additionally, 6 were oxyimino-cephalosporin-resistant. The qnrB, qnrS1, and blaCTX-M-14 genes were detected, whereas no isolates carried the mcr-1 gene. Isolates had the ability to adhere to Hep-2 cells and form biofilms. Only 1 isolate expressed toxins in vitro. E. coli from NCD cases in Uruguay are very diverse, potentially virulent, and may interact with eukaryotic cells. Zoonotic potential, together with resistance traits and the presence of horizontal transfer mechanisms, may play a significant role in infections caused by these microorganisms.

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Section: Discussioncontrasting

confidence: 92%

Zoonotic Potential and Antibiotic Resistance of <i>Escherichia coli</i> in Neonatal Calves in Uruguay

et al. 2017

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“…Vaccines commercially available for BVDV prevention in Uruguay are manufactured with inactivated virus and can only provide partial protection, unlike modified-live vaccines used in other regions [56], including South American countries such as Brazil [57]. Recent investigations in Argentina have led to developing an enhanced BVDV subunit vaccine expressed in a baculovirus, based on a truncated E2 glycoprotein fused to a single-chain antibody that targets antigenpresenting cells [58]. This vaccine's immunogenicity was tested in guinea pigs and cattle, and immunized cattle developed high levels of neutralizing antibodies against BVDV up to 1 year after immunization.…”

Section: Discussionmentioning

confidence: 99%

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

et al. 2019

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Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric coinfection with coccidia, and transplacental fetal infections and abortions with Neospora caninum coinfection. BVDV-1a and BVDV-2b were each identified in four of the eight cases. We conclude that BVDV-1a and BVDV-2b contribute significantly to disease and mortality in cattle in Uruguay. Future research should estimate the economic impact of BVDV in the Uruguayan livestock sector.

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“…However, the targeting of molecules expressed concurrently by several antigen presenting cell types has also led to improved antibody responses in different species. This is the case of MHC class II targeting, as demonstrated in pigs 63 , in cows 64 and recently by some of us in pigs and ferrets 65 . Indeed MHC class II-targeting may operate through promoting positive interactions between the targeted subsets, such as DC and B cells which express the molecule 66 .…”

Section: Discussionmentioning

confidence: 72%

The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin

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Barnier-Quer

et al. 2017

Sci Rep

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XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and veterinary applications. Here we evaluated in pigs the immunogenicity of bivalent protein structures made of XCL1 fused to the external portion of the influenza virus M2 proton pump, which is conserved through strains and a candidate for universal influenza vaccines. Pigs represent a relevant target of such universal vaccines as pigs can be infected by swine, human and avian strains. We found that cDC1 were the only cell type labeled by XCR1-targeted mCherry upon intradermal injection in pig skin. XCR1-targeted M2e induced higher IgG responses in seronegative and seropositive pigs as compared to non-targeted M2e. The IgG response was less significantly enhanced by CpG than by XCR1 targeting, and CpG did not further increase the response elicited by XCR1 targeting. Monophosphoryl lipid A with neutral liposomes did not have significant effect. Thus altogether M2e-targeting to XCR1 shows promises for a trans-species universal influenza vaccine strategy, possibly avoiding the use of classical adjuvants.

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Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells

Cited by 7 publications

References 15 publications

Zoonotic Potential and Antibiotic Resistance of <i>Escherichia coli</i> in Neonatal Calves in Uruguay

Zoonotic Potential and Antibiotic Resistance of <i>Escherichia coli</i> in Neonatal Calves in Uruguay

Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay

The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin

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