Development of an enhanced chain elongation process for caproic acid production from waste-derived lactic acid and butyric acid

Nzeteu, Corine; Coelho, Fabiana Magalhães; Trego, Anna Christine; Abram, Florence; Ramiro-García, Javier; Paulo, Lara M.; O’Flaherty, Vincent

doi:10.1016/j.jclepro.2022.130655

Cited by 25 publications

(12 citation statements)

References 38 publications

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“…Clearly, the presence of butyric acid in silage F enhanced the formation of MCCs during fermentation, probably because the acid, which is also an ED, 43 is better utilized for microbial CE than lactic acid or ethanol. Nzeteu et al 7 . also reported a higher yield of MCCs from waste‐derived substrates in the presence of butyric acid.…”

Section: Resultsmentioning

confidence: 94%

“…In the present study, the pathway for the production of even‐numbered MCCs was preferred to the pathway for the production of odd‐numbered MCCs, as seen from the higher concentration of caproic acid (C6) and caprylic acid (C8) in comparison with heptanoic acid (C7). After 28 days of fermentation, the fermentation broths contained mainly acetic acid (36.58%), butyric acid (18.91%), caproic acid (15.10%), and caprylic acid (4.02%) from silage A, and acetic acid (35.79%), butyric acid (27.86%), caproic acid (22.18%), and caprylic acid (8.48%) from silage F. Many authors have also reported the dominance of acetic acid during the production of MCCs irrespective of the substrate or inoculum used 7,38 . Two reasons have been given for the dominance of acetic acid in the fermentation of waste streams: (i) abundance of acetic acid‐producing bacteria in the inoculum and (ii) excessive oxidation of ethanol 9 .…”

Section: Resultsmentioning

confidence: 99%

“…The production of higher concentrations of even-numbered MCCs than odd-numbered MCCs in the present study is consistent with the report of Wu et al 42 Clearly, the presence of butyric acid in silage F enhanced the formation of MCCs during fermentation, probably because the acid, which is also an ED, 43 is better utilized for microbial CE than lactic acid or ethanol. Nzeteu et al 7 also reported a higher yield of MCCs from waste-derived substrates in the presence of butyric acid. Despite the high concentration of MCCs produced, the high concentration of butyric acid after fermentation of both silages is an indication that the ensiling and CE process could be improved.…”

Section: Medium-chain Fatty Acid Production From Silagesmentioning

confidence: 95%

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Medium chain carboxylate production from cassava wastes pretreated by ensiling

Undiandeye

Kiman

Abubakar

et al. 2023

Biofuels Bioprod Bioref

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Every year, approximately 35 million tons of combined cassava peels (CP) and cassava wastewater (CWW) are generated from the processing of cassava in Nigeria. The improper disposal of these wastes has serious environmental consequences in the form of air and water pollution. Using these wastes for biofuel production is one way of mitigating the impact of the mass flows on the environment. However, being a lignocellulosic biomass, CP requires pretreatment before it can yield a reasonable amount of biofuel. In this study, ensiling was chosen as a method for the pretreatment of CP slurry (CPS), formed from the combination of CP and CWW, for the production of medium-chain carboxylates (MCCs), which have been reported to be more valuable than biogas and bioethanol. Calcined egg shells (CES) were added to some of the CPS before ensiling, to enhance the formation of butyric acid. The MCCs were produced through batch fermentation of the silage. The results show that ensiling degraded the hemicellulose and cellulose content of CPS. The silage containing CES had greater loss of volatile solids, higher butyric acid content, and a higher MCCs (C6-C8) yield of 210 gkg -1 VS while the silage without CES had an MCC yield of 137 gkg -1 VS. Out of the three kinetic models that were used to predict the production of MCCs, the dual pool model gave the best prediction.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Medium-chain Fatty Acid Production From Silagesmentioning

confidence: 95%

See 1 more Smart Citation

Medium chain carboxylate production from cassava wastes pretreated by ensiling

Undiandeye

Kiman

Abubakar

et al. 2023

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

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“…Meanwhile, a mixture of fermentation products from a wide range of carbohydrates is produced by Bacteroidaceae , Prevotellaceae and Lachinospiraceae can be directly associated with the VFA components especially acetate, butyrate, propionate, and caproate, with many species members active in the fermentation of carbohydrates present in the food waste . Finally, it is also noteworthy that many members of bacterial families such as Prevotellaceae , Ruminococcaceae , Clostridiaceae , and Lachnospiraceae are reported to perform homoacetogenesis resulting in the dominant acetic acid production at alkaline pH …”

Section: Discussionmentioning

confidence: 99%

“…Novel strategies are being developed, such as bioaugmentation for targeted tailor-made VFA production . Recently, the chain elongation process that occurs via the reversed β-oxidation pathway and the fatty acid biosynthesis pathway to produce less hydrophilic medium chain carboxylic acids (C 6 –C 12 ) through secondary fermentation of short-chain organic acids has gained interest as well . VFAs can be developed for end-use applications such as alcohol, bioplastics, biodiesel, biological nutrient recovery, and electricity generation .…”

Section: Discussionmentioning

confidence: 99%

Influence of pH, Heat Treatment of Inoculum, and Selenium Oxyanions on Concomitant Selenium Bioremediation and Volatile Fatty Acid Production from Food Waste

Logan,

Zhu,

Lens

et al. 2023

ACS Omega

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Developing novel strategies to enhance volatile fatty acid (VFA) yield from abundant waste resources is imperative to improve the competitiveness of biobased VFAs over petrochemical-based VFAs. This study hypothesized to improve the VFA yield from food waste via three strategies, viz., pH adjustment (5 and 10), supplementation of selenium (Se) oxyanions, and heat treatment of the inoculum (at 85 °C for 1 h). The highest VFA yield of 0.516 g COD/g VS was achieved at alkaline pH, which was 45% higher than the maximum VFA production at acidic pH. Heat treatment resulted in VFA accumulation after day 10 upon alkaline pretreatment. Se oxyanions acted as chemical inhibitors to improve the VFA yield at pH 10 with non-heat-treated inoculum (NHT). Acetic and propionic acid production was dominant at alkaline pH (NHT); however, the VFA composition diversified under the other tested conditions. More than 95% Se removal was achieved on day 1 under all the conditions tested. However, the heat treatment was detrimental for selenate reduction, with less than 15% Se removal after 20 days. Biosynthesized Se nanoparticles were confirmed by transmission and scanning electron microscopy and and energy dispersive X-ray analyses. The heat treatment inhibited the presence of nonsporulating bacteria and methanogenic archaea (Methanobacteriaceae). High-throughput sequencing also revealed higher relative abundances of the bacterial families (such as Clostridiaceae, Bacteroidaceae, and Prevotellaceae) that are capable of VFA production and/or selenium reduction.

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