Development of an Enzyme Immunoassay for the Determination of the Herbicide Metsulfuron-Methyl Based on Chicken Egg Yolk Antibodies

Welzig, Elvira; Pichler, Harald; Krska, Rudolf; Knopp, Dietmar

doi:10.1080/03067310008041347

Cited by 7 publications

(6 citation statements)

References 13 publications

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“…The molar ratio of hapten:proteins used for the conjugation reactions was 100:1. The mixtures were stirred at room temperature for 3 hr and dialyzed against PBS solution for 3 days (Misaki et al, 2019;Welzig et al, 2000). The products were stored at −20 • C. The method of preparation of the enzyme tracer was the same as above.…”

Section: Preparation Of Immunogens and The Enzyme Tracermentioning

confidence: 99%

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Liu

Qin

Cao

et al. 2021

Journal of Food Science

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A hapten of metsulfuron‐methyl was successfully designed and synthesized from 2‐methylester‐phenylsulfonamide and succinic anhydride, and the polyclonal antibody against metsulfuron‐methyl was prepared by immunization procedure with the hapten–bovine serum albumin conjugate. A stable and sensitive direct competitive enzyme‐linked immunosorbent assay (dcELISA) method had been developed under the optimal conditions. The sensitivity (IC50) was 37.03 ± 1.87 µg/L, and the detection line (IC15) was 1.57 ± 0.11 µg/L. Rice, wheat, oat, flaxseed, milk, and water were chosen to study the recovery test and the recovery rates were 83.11%–117.44% . The matrix effect was eliminated by a simple dilution of the sample extracts. The results from dcELISA were well agreement with the results from HPLC–MS. It was indicated that the developed method had good accuracy and stability. It could be applied for the detection of metsulfuron‐methyl residues. It was worth mentioning that the antibody could recognize metsulfuron‐methyl and tribenuron‐methyl with cross‐reactivities of 100% and 49.72%, respectively. In order to understand the cross‐reactivity, molecular modeling including molecular alignment and electrostatic potential surfaces were introduced. It was found that the special group of metsulfuron‐methyl played an important role, especially on C3 position of the phenyl group. Practical Application A stable, sensitive, and low‐cost dc ELISA method had been developed with good accuracy and applied in the determination of metsulfuron‐methyl in foods. Molecular simulation was introduced to understand the specificity between the antibody and the analyst. It was a good method to study the cross‐reactivity between the antibody and the analyst or analogue.

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Section: Preparation Of Immunogens and The Enzyme Tracermentioning

confidence: 99%

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Liu

Qin

Cao

et al. 2021

Journal of Food Science

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“…Hapten design considerations include the position of the active site group and the length of the connecting spacer arm, as well as the stability and solubility of the hapten [7][8][9][10]. Due to the instability of sulfonylureas, a common strategy for hapten synthesis involves adding a functional spacer arm to a stable metabolite of sulfonylurea [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the addition of a succinic acid spacer arm to stable metabolites of triasulfuron, metsulfuron-methyl, and chlorimuron-ethyl resulted in the production of monoclonal antibodies (mAbs) with enhanced sensitivity [11][12][13]. These antibodies were then applied in the development of highly sensitive indirect competitive ELISAs for target molecule detection.…”

Section: Introductionmentioning

confidence: 99%

Development of Magnetic Lateral Flow and Direct Competitive Immunoassays for Sensitive and Specific Detection of Halosulfuron-Methyl Using a Novel Hapten and Monoclonal Antibody

Ying

Cui

et al. 2023

Foods

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Halosulfuron-methyl (HM) is widely used for the removal of noxious weeds in corn, sugarcane, wheat, rice, and tomato fields. Despite its high efficiency and low toxicity, drift to nontarget crops and leaching of its metabolites to groundwater pose potential risks. Considering the instability of HM, the pyrazole sulfonamide of HM was used to generate a hapten and antigen to raise a high-quality monoclonal antibody (Mab, designated 1A91H11) against HM. A direct competitive immunoassay (dcELISA) using Mab 1A91H11 achieved a half-maximal inhibitory concentration (IC50) of 1.5 × 10−3 mg/kg and a linear range of 0.7 × 10−3 mg/kg–10.7 × 10−3 mg/kg, which was 10 times more sensitive than a comparable indirect competitive ELISA (icELISA) and more simple to operate. A spiking recovery experiment performed in tomato and maize matrices with 0.01, 0.05, and 0.1 mg/kg HM had average recoveries within 78.9–87.9% and 103.0–107.4% and coefficients of variation from 1.1–6.8% and 2.7–6.4% in tomato and maize, respectively. In addition, a magnetic lateral flow immunoassay (MLFIA) was developed for quantitative detection of low concentrations of HM in paddy water. Compared with dcELISA, the MLFIA exhibited 3.3- to 50-fold higher sensitivity (IC50 0.21 × 10−3 mg/kg). The average recovery and RSD of the developed MLFIA ranged from 81.5 to 92.5% and 5.4 to 9.7%. The results of this study demonstrated that the developed dcELISA and MLFIA are suitable for rapid detection of HM residues in tomato and maize matrices and paddy water, respectively, with acceptable accuracy and precision.

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“…High performance liquid chromatography (HPLC) with ultraviolet detector or diode array detector was the most common approaches [10,11], other methods such as liquid chromatography with mass spectrometry [12], capillary electrophoresis [13], supercritical fluid chromatography [14], bioassay [15] and enzyme immunoassay [16] are often found in the published reports. Triasulfuron was determined in soil by liquid chromotography with UV detection [17] or mass spectrometric detection [18].…”

Section: Introductionmentioning

confidence: 99%

A direct method for the polarographic determination of herbicide triasulfuron and application to natural samples and agrochemical formulation

Sarıgül

İnam

2009

Bioelectrochemistry

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Development of an Enzyme Immunoassay for the Determination of the Herbicide Metsulfuron-Methyl Based on Chicken Egg Yolk Antibodies

Cited by 7 publications

References 13 publications

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Development of Magnetic Lateral Flow and Direct Competitive Immunoassays for Sensitive and Specific Detection of Halosulfuron-Methyl Using a Novel Hapten and Monoclonal Antibody

A direct method for the polarographic determination of herbicide triasulfuron and application to natural samples and agrochemical formulation

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