2015
DOI: 10.1002/jssc.201500242
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Development of an enzyme‐linked immunosorbent assay and immunoaffinity chromatography for glycyrrhizic acid using an anti‐glycyrrhizic acid monoclonal antibody

Abstract: In this work, a new monoclonal antibody specific for glycyrrhizic acid was prepared and characterized. A hybridoma secreting an anti-glycyrrhizic acid monoclonal antibody was produced by fusing splenocytes from a mouse immunized against a glycyrrhizic acid-bovine serum albumin conjugate with the hypoxanthine-aminopterin-thymidine-sensitive mouse myeloma cell line (Sp2/0-Ag14). Subsequently, an indirect, competitive enzyme-linked immunosorbent assay for glycyrrhizic acid was developed using the monoclonal antib… Show more

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Cited by 11 publications
(18 citation statements)
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“…Our results indicate that the IAC can efficiently separate the Nar from many similar flavonoids, including geometric isomers in Citrus aurantium . Additionally, in our previous work, we also separated glycyrrhizic acid from Glycyrrhiza uralensis and resolved 20( S )‐GsRg2 and 20( R )‐GsRg2 using IAC . Other researchers have also reported the application of IAC in the separation of natural products , residual pesticides , and fungal toxin .…”
Section: Resultsmentioning
confidence: 77%
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“…Our results indicate that the IAC can efficiently separate the Nar from many similar flavonoids, including geometric isomers in Citrus aurantium . Additionally, in our previous work, we also separated glycyrrhizic acid from Glycyrrhiza uralensis and resolved 20( S )‐GsRg2 and 20( R )‐GsRg2 using IAC . Other researchers have also reported the application of IAC in the separation of natural products , residual pesticides , and fungal toxin .…”
Section: Resultsmentioning
confidence: 77%
“…The hapten–protein conjugate may provoke an adequate immune response . In this work, Nar–BSA conjugate was synthesized using a periodate oxidation procedure based on a method reported previously with some modifications . Briefly, 50 mg of Nar was dissolved in double distilled water to a concentration of 1 mg/mL.…”
Section: Methodsmentioning
confidence: 99%
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“…ELISA is an immunochemical assay technique that is used to quantitatively or qualitatively detect the presence of a specific protein [76, 126128]. A typical method for detecting antigen in the sample is sandwich ELISA.…”
Section: Bioanalytical Methods For Polyethylene Glycols and Pegylatedmentioning
confidence: 99%