Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Tyramine as an Index of Freshness in Meat and Seafood

Sheng, Wei; Sun, Congcong; Fang, Guozhen; Wu, Xuening; Hu, Gaoshuang; Zhang, Yan; Wang, Shuo

doi:10.1021/acs.jafc.6b04422

Cited by 32 publications

(9 citation statements)

References 29 publications

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“…Enzyme-linked immunosorbent assay (ELISA) is mainly based on the highly specific antigen–antibody recognition and the efficient biocatalytic property of an enzyme. , By virtue of the excellent specificity, low cost, and straightforward readout, ELISA has already become the most widely used format of immunoassay in clinical diagnosis, − foods quality control, , environmental monitoring, and in laboratory research . Alkaline phosphatase (ALP) is extensively used as a labeling enzyme in ELISA to produce detectable signals because of its high catalytic activity, broad substrate specificity, easy conjugation to antibodies, mild reaction conditions, and good stability .…”

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confidence: 99%

Fluorescence Immunoassay Based on the Phosphate-Triggered Fluorescence Turn-on Detection of Alkaline Phosphatase

et al. 2018

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A simple and cost-effective fluorescence immunoassay for the sensitive quantitation of disease biomarker α-fetoprotein (AFP) has been developed based on the phosphate-triggered fluorescence turn-on detection of alkaline phosphatase (ALP), with the reversible binding between calcein and Ce as a signaling element. In this immunoassay, fluorescent calcein is readily quenched by Ce via a coordination process. The ALP-catalyzed hydrolysis of p-nitrophenyl phosphate leads to the formation of p-nitrophenol and inorganic orthophosphate, and the newly formed orthophosphate could potently combine with Ce due to the higher affinity, thus, recovering the fluorescence of calcein. The corresponding fluorescence signal triggered by phosphate is related to ALP activities labeled on antibody, and thus could be applied to detect target antigen in an enzyme-linked immunosorbent assay (ELISA) platform. The fluorescence intensity correlated well to the AFP concentration ranges of 0.2-1.0 and 1.0-4.0 ng/mL, with a detection limit of 0.041 ng/mL. The proposed fluorescence ELISA possesses convincing recognition mechanism and exhibits excellent assay performance in the evaluation of the AFP level in serologic test, which unambiguously reveals great application potential in the clinic diagnosis of disease biomarkers.

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confidence: 99%

Fluorescence Immunoassay Based on the Phosphate-Triggered Fluorescence Turn-on Detection of Alkaline Phosphatase

et al. 2018

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“…The animal experiments were performed in compliance with the Regulations for the Administration of Affairs Concerning Experimental Animals (Ministry of Science and Technology of the People's Republic of China, 2006). Polyclonal antibodies were obtained by immunizing the New Zealand white rabbits (Liu et al., 2017; Song et al., 2016; Sheng et al., 2016; Sheng et al., 2016; Xu et al., 2014; Zhang et al., 2018). The hapten–BSA, hapten–KLH, and hapten–OVA were diluted to 1 mg/ml with a 0.9% NaCl solution and then emulsified with Freund's adjuvant (1:1) before immunization of the rabbits.…”

Section: Methodsmentioning

confidence: 99%

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Liu

Qin

Cao

et al. 2021

Journal of Food Science

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A hapten of metsulfuron‐methyl was successfully designed and synthesized from 2‐methylester‐phenylsulfonamide and succinic anhydride, and the polyclonal antibody against metsulfuron‐methyl was prepared by immunization procedure with the hapten–bovine serum albumin conjugate. A stable and sensitive direct competitive enzyme‐linked immunosorbent assay (dcELISA) method had been developed under the optimal conditions. The sensitivity (IC50) was 37.03 ± 1.87 µg/L, and the detection line (IC15) was 1.57 ± 0.11 µg/L. Rice, wheat, oat, flaxseed, milk, and water were chosen to study the recovery test and the recovery rates were 83.11%–117.44% . The matrix effect was eliminated by a simple dilution of the sample extracts. The results from dcELISA were well agreement with the results from HPLC–MS. It was indicated that the developed method had good accuracy and stability. It could be applied for the detection of metsulfuron‐methyl residues. It was worth mentioning that the antibody could recognize metsulfuron‐methyl and tribenuron‐methyl with cross‐reactivities of 100% and 49.72%, respectively. In order to understand the cross‐reactivity, molecular modeling including molecular alignment and electrostatic potential surfaces were introduced. It was found that the special group of metsulfuron‐methyl played an important role, especially on C3 position of the phenyl group. Practical Application A stable, sensitive, and low‐cost dc ELISA method had been developed with good accuracy and applied in the determination of metsulfuron‐methyl in foods. Molecular simulation was introduced to understand the specificity between the antibody and the analyst. It was a good method to study the cross‐reactivity between the antibody and the analyst or analogue.

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“…New Zealand white rabbits were selected for the broad-spectrum antibody production . The detailed immunization steps are listed in the Supporting Information.…”

Section: Materials and Methodsmentioning

confidence: 99%

“…New Zealand white rabbits were selected for the broad-spectrum antibody production. 36 The detailed immunization steps are listed in the Supporting Information. The animal experiments were implemented in accordance with "Administrative Regulations on Laboratory Animal Affairs" promulgated by the Ministry of Science and Technology of the People's Republic of China and the Tianjin Municipal Science and Technology Bureau.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Preparation of a Broad-Spectrum Heterocyclic Aromatic Amines (HAAs) Antibody and Its Application in Detection of Eight HAAs in Heat Processed Meat

Sheng

Zhang

Zhao

et al. 2020

J. Agric. Food Chem.

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Heterocyclic aromatic amines (HAAs) are potential human mutagens and carcinogens mainly generated in heat-treated meat. In this work, a broad-spectrum HAAs antibody was prepared and used to develop an indirect competitive ELISA (ic-ELISA) for simultaneous determination of eight HAAs, including 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3,4-dimethylimidazo[4,5-f] quinoline (MeIQ), 2-amino-3-methylimidazo[4,5-f]quinoxaline (IQx), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx), 2-amino-3,7,8-trimethylimidazo[4,5-f]quinoxaline (7,8-DiMeIQx), 2-amino-3,4,7,8-tetramethylimidazo[4,5-f]quinoxaline (4,7,8-TriMeIQx), and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in grilled and fried meat samples. The limit of detection (LOD, calculated as IC10) and 50% inhibition concentration (IC50) of ic-ELISA were 5.29 μg/L and 99.08 μg/L, respectively. The detection results of this ic-ELISA were in good agreement with the detection results of UPLC-MS/MS in real samples, which indicated that this ic-ELISA can be applied to detect the total content of eight HAAs in heat processed meat. Use of a broad-spectrum antibody is an efficient strategy in developing immunoassay for simultaneous measuring food risk factors with similar structure.

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Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Tyramine as an Index of Freshness in Meat and Seafood

Cited by 32 publications

References 29 publications

Fluorescence Immunoassay Based on the Phosphate-Triggered Fluorescence Turn-on Detection of Alkaline Phosphatase

Fluorescence Immunoassay Based on the Phosphate-Triggered Fluorescence Turn-on Detection of Alkaline Phosphatase

A stable and sensitive enzyme‐linked immunosorbent assay (ELISA) for the determination of metsulfuron‐methyl residues in foods

Preparation of a Broad-Spectrum Heterocyclic Aromatic Amines (HAAs) Antibody and Its Application in Detection of Eight HAAs in Heat Processed Meat

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