Development of an Enzyme-Linked Immunosorbent Assay Based on CD150/SLAM for the Detection of Peste des Petits Ruminant Virus

Abstract

Peste des petits ruminant (PPR) is an economically important severe viral disease of small ruminants that affects primarily the respiratory and digestive tract. Specific detection of the PPR virus (PPRV) antigen plays an important role in the disease control and eradication program. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant goat signaling lymphocyte activation molecule (SLAM) as the capture ligand was successfully developed for the detection of the PPRV antigen (PPRV SLAM-iELISA). The assay was highly specific for PPRV with no cross-reactions among foot and mouth disease virus, Orf virus, sheep pox virus, and goat pox virus and had a sensitivity with a detection limit of 1.56 × 10 1 TCID 50 /reaction (50 µl). Assessment of 136 samples showed that the developed PPRV SLAM-iELISA was well correlated with real-time RT-qPCR assays and commercially available sandwich ELISA for detection of PPRV and showed relative sensitivity and specificity of 93.75 and 100.83%, respectively. These results suggest that the developed PPRV SLAM-iELISA is suitable for specific detection of the PPRV antigen. This study demonstrated for the first time that the goat SLAM, the cellular receptor for PPRV, can be used for the development of a diagnostic method for the detection of PPRV.

Prediction of the Potential Host of Peste Des Petits Ruminants Virus by the Least Common Amino Acid Pattern in SLAM Receptor

“…They also had a similar mechanism for transmitting between species and adapting to new hosts [53][54][55]. SLAM is the principal active receptor for PPRV binding to host cells, and many studies have demonstrated that SLAM was closely related to host susceptibility and species specificity of PPRV infection [31,56,57]. Moreover, Nectin-4 involves an epithelial receptor with less interaction with the host specificity of PPRV [58].…”

Research Progress on Emerging Viral Pathogens of Small Ruminants in China during the Last Decade

“…An indirect ELISA based on the recombinant goat signaling lymphocyte activation molecule (SLAM) as the capture ligand was successfully developed for the detection of the PPRV antigen. The assay was highly specific for PPRV with no cross-reactions to other viruses [42]. In addition, a simplex suspension array was also established, with a detection limit of 5.78 × 10 4 copies/mL, and it was shown to be 100% consistent with the conventional RT-PCR [43].…”