Development of an equipment free paper based fluorimetric method for the selective determination of histidine in human urine samples

Tarara, Maria; Tsiasioti, Apostolia; Tzanavaras, Paraskevas D.; Tsogas, George Z.

doi:10.1016/j.talanta.2022.123685

Cited by 8 publications

(12 citation statements)

References 49 publications

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“…The main advantage of analytical paper devices is their portability in the field, and from our previous report [ 38 ], we have decided that the order of addition of the reagents is considered a key parameter to determine the manner and order of their deposition on the surface of the paper to manufacture suitable sensors for analytical determinations. It is a given that in all field analyses, the amount of analyte is added at the end of the experimental process, as all the reactants should be present and then the determination of the analyte be made.…”

Section: Resultsmentioning

confidence: 99%

“…OPA is an established derivatization reagent for the fluorimetric determination of GSH [ 8 , 37 ]. Reduced GSH reacts with OPA to form a stable, highly fluorescent tricyclic derivate at pH 8, while other essential amino acids like L-histidine (His) react at a higher alkaline environment [ 38 ]. Additionally, paper-based analytical devices (PADs) have been developed in the past few years, at extremely fast rates, as alternative platforms for the determination of thiols based on their significant advantages, including low cost, portability, and minimal instrumentation demands [ 38 , 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

“…Reduced GSH reacts with OPA to form a stable, highly fluorescent tricyclic derivate at pH 8, while other essential amino acids like L-histidine (His) react at a higher alkaline environment [ 38 ]. Additionally, paper-based analytical devices (PADs) have been developed in the past few years, at extremely fast rates, as alternative platforms for the determination of thiols based on their significant advantages, including low cost, portability, and minimal instrumentation demands [ 38 , 39 , 40 ]. Moreover, electronic imaging devices including flatbed scanners [ 41 ] and smartphones [ 42 , 43 ] have been used in the last few years for the analytical determination of GSH.…”

Section: Introductionmentioning

confidence: 99%

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O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

Tarara,

Tzanavaras,

Tsogas

2024

Molecules

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Herein, a new, direct paper-based fluorimetric method is described for the quantitative determination of glutathione (GSH) molecules in nutritional supplements. Briefly, the proposed analytical method is based on the fluorescence emission resulting from the direct and selective chemical reaction of GSH molecules with the derivatization reagent that is o-phthalaldehyde (OPA) in acidic conditions at room temperature. The intensity of the emitted fluorescence on the surface of the analytical paper devices after irradiation with a lamp at 365 nm is proportional to the concentration of GSH and is measured using a smartphone as the detector. This methodology, which is suitable for measurements in laboratories with limited resources, does not require specialized instrumentation or trained personnel. The protocol governing the proposed method is simple and easily applicable. Essentially, the chemical analyst should adjust the value of pH on the surface of the paper by adding a minimal amount of buffer solution; then, after adding a few microliters of the derivatization reagent, wait for the surface of the paper to dry and, finally, add the analyte. Subsequently, the irradiation of the sensor and the measurement of the emitted fluorescence can be recorded with a mobile phone. In the present study, several parameters affecting the chemical reaction and the emitted fluorescence were optimized, the effect of interfering compounds that may be present in dietary supplements was examined, and the stability of these paper sensors under different storage conditions was evaluated. Additionally, the chemical stability of these paper devices in various maintenance conditions was studied, with satisfactory results. The detection limit calculated as 3.3 S/N was 20.5 μmol L−1, while the precision of the method was satisfactory, ranging from 3.1% (intra-day) to 7.3% (inter-day). Finally, the method was successfully applied to three different samples of dietary supplements.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

Tarara,

Tzanavaras,

Tsogas

2024

Molecules

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“…In this research work, the paper substrate was used for reagent storage and also volume limitation with the exploitation of hydrophobic barriers, and the signal has been acquired through the use of a conventional smartphone. 75 Another approach of a simple pattern fluorometric paper-based device exploits carbon dots (CDs) nanoparticles (NPs) properties with the platform's surface functionalization for folic acid detection in urine down to 0.28 μM as the detection limit (Figure 7B). The sustainable paper-based substrate that was used for the CDs fluorophore anchoring was attached on the PDMS plate providing an ultimate cost-effective hybrid sensing platform.…”

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confidence: 99%

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confidence: 99%

Paper-Based Materials for Diagnostics

Kalligosfyri,

Cinti

2024

ACS Materials Lett.

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Nowadays point-of care (POC) devices dominate the field of bioanalysis as they play a pivotal role in improving different aspects of diagnostics including screening, early diagnosis, and disease monitoring. These devices comply with the World Health Organization ASSURED criteria that describe the ideal POC tool. While conventional materials like polymers, silicon, metallic foils, and glass have been exploited in POC device manufacturing, recent innovations relying on paper-based materials have introduced a new era of versatile diagnostic tools. Because of their properties, cellulose and its derivatives have emerged as the most common paper-based substrate in device fabrication. The present review explores recent developments in paper-based diagnostics, covering a wide range of applications including reagent storage and isolation/extraction of the analyte and also serving as a sensing platform. The versatility of paper substrates in various diagnostic devices such as lateral flow assays, electrochemical sensors, microfluidics, etc. is discussed, highlighting their advantages, challenges, and limitations on the context of precision medicine.

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Current trends in digital camera-based bioassays for point-of-care tests

Pohanka

2024

Clinica Chimica Acta

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Development of an equipment free paper based fluorimetric method for the selective determination of histidine in human urine samples

Cited by 8 publications

References 49 publications

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

O-Phthalaldehyde Derivatization for the Paper-Based Fluorometric Determination of Glutathione in Nutritional Supplements

Paper-Based Materials for Diagnostics

Current trends in digital camera-based bioassays for point-of-care tests

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