Development of an estrogen-dependent breast cancer co-culture model as a tool for studying endocrine disruptors

Yancu, Debbie; Viau, Rachel; Sanderson, Thomas H.

doi:10.1016/j.tiv.2019.104658

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…Finally, new methods of co-culture of breast tumor cells with adipocytes or tumor-associated macrophages (TAMs) have been recently developed and presented in the literature, as a step forward in mimicking in vivo conditions. − For example, the study by Wang and colleagues demonstrated that the viability of breast tumor cells (MDA-MB-231 and T41) was highly reduced after treatment with an anti-mammary hyperplasia drug, when grown in co-cultures with TAMs . In this study, we developed a noncontact co-culture system involving tumor and/or nontumoral breast cell lines, in which a permeable membrane allowed free exchange of media and soluble molecules.…”

Section: Discussionmentioning

confidence: 99%

Collagen-Binding Nanoparticles for Paclitaxel Encapsulation and Breast Cancer Treatment

Passos,

Lopes,

Panitch

2023

ACS Biomater. Sci. Eng.

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In this study, we developed a novel hybrid collagen-binding nanocarrier for potential intraductal administration and local breast cancer treatment. The particles were formed by the encapsulation of nanostructured lipid carriers (NLCs) containing the cytotoxic drug paclitaxel within a shell of poly( N -isopropylacrylamide) (pNIPAM), and were functionalized with SILY, a peptide that binds to collagen type I (which is overexpressed in the mammary tumor microenvironment) to improve local retention and selectivity. The encapsulation of the NLCs in the pNIPAM shell increased nanoparticle size by approximately 140 nm, and after purification, a homogeneous system of hybrid nanoparticles (∼96%) was obtained. The nanoparticles exhibited high loading efficiency (<76%) and were capable of prolonging paclitaxel release for up to 120 h. SILY-modified nanoparticles showed the ability to bind to collagen-coated surfaces and naturally elaborated collagen. Hybrid nanoparticles presented cytotoxicity up to 3.7-fold higher than pNIPAM-only nanoparticles on mammary tumor cells cultured in monolayers. In spheroids, the increase in cytotoxicity was up to 1.8-fold. Compared to lipid nanoparticles, the hybrid nanoparticle modified with SILY increased the viability of nontumor breast cells by up to 1.59-fold in a coculture model, suggesting the effectiveness and safety of the system.

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Section: Discussionmentioning

confidence: 99%

Collagen-Binding Nanoparticles for Paclitaxel Encapsulation and Breast Cancer Treatment

Passos,

Lopes,

Panitch

2023

ACS Biomater. Sci. Eng.

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“…Overall, the HT-H295R assay, with the ANOVA-based approach for assigning a hit-call, currently provides the best available in vitro prediction of effects on E2 and P4 synthesis in a HT format. New coculture models may provide even better breast cancer-relevant models for testing EDCs, for example, by increasing the sensitivity of E2-up responses, integrating measures of breast cell proliferation and aromatase activation, and by incorporating more relevant aromatase promoters (Yancu et al 2020).…”

Section: Strengths and Limitations Of In Vitro Data From The H295r Steroidogenesis Assaymentioning

confidence: 99%

Application of an in Vitro Assay to Identify Chemicals That Increase Estradiol and Progesterone Synthesis and Are Potential Breast Cancer Risk Factors

Cardona

Rudel

2021

Environ Health Perspect

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BACKGROUND: Established breast cancer risk factors, such as hormone replacement therapy and reproductive history, are thought to act by increasing estrogen and progesterone (P4) activity. OBJECTIVE: We aimed to use in vitro screening data to identify chemicals that increase the synthesis of estradiol (E2) or P4 and evaluate potential risks. METHOD: Using data from a high-throughput (HT) in vitro steroidogenesis assay developed for the U.S. Environmental Protection Agency (EPA) ToxCast program, we identified chemicals that increased estradiol (E2-up) or progesterone (P4-up) in human H295R adrenocortical carcinoma cells. We prioritized chemicals by their activity. We compiled in vivo studies and assessments about carcinogenicity and reproductive/developmental (repro/dev) toxicity. We identified exposure sources and predicted intakes from the U.S. EPA's ExpoCast. RESULTS: We found 296 chemicals increased E2 (182) or P4 (185), with 71 chemicals increasing both. In vivo data often showed effects consistent with this mechanism. Of the E2-and P4-up chemicals, about 30% were likely repro/dev toxicants or carcinogens, whereas only 5-13% were classified as unlikely. However, most of the chemicals had insufficient in vivo data to evaluate their effects. Of 45 chemicals associated with mammary gland effects, and also tested in the H294R assay, 29 increased E2 or P4, including the well-known mammary carcinogen 7,12-dimethylbenz(a)anthracene. E2-and P4-up chemicals include pesticides, consumer product ingredients, food additives, and drinking water contaminants. DISCUSSION: The U.S. EPA's in vitro screening data identified several hundred chemicals that should be considered as potential risk factors for breast cancer because they increased E2 or P4 synthesis. In vitro data is a helpful addition to current toxicity assessments, which are not sensitive to mammary gland effects. Relevant effects on the mammary gland are often not noticed or are dismissed, including for 2,4-dichlorophenol and cyfluthrin. Fifty-three active E2-up and 59 active P4-up chemicals that are in consumer products, food, pesticides, or drugs have not been evaluated for carcinogenic potential and are priorities for study and exposure reduction.

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“…These results were in harmony with an in vitro study done by Sreeja S et al [ 13 ], which investigated the fenugreek effects and its capacity as an alternative to hormone replacement therapy and concluded that fenugreek stimulated the proliferation of MCF-7 breast cancer cells. In addition, Yancu D et al [ 14 ] studied the effects of fennel seeds on the steroidogenesis of the tumor, which stimulated mammary tumor cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Attitudes, Beliefs, and Potential Effects of Nutritional Phytoestrogenic Plant Consumption on Women’s Health in Saudi Arabia

Elagib,

Alshammari,

Alsadoon

et al. 2023

Cureus

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Background and objective: Phytoestrogens are plant-derived endocrine-disrupting chemicals known as estrogen-like compounds with health and preventive benefits. This study aimed to assess Saudi Arabian women's beliefs and attitudes toward phytoestrogen-rich plant consumption and its association with hormone-sensitive tumors and diseases.Methods: A cross-sectional study was carried out between November 2022 and May 2023 in Saudi Arabia using an online self-administered questionnaire created via Google Forms (Appendix). The collected data were extracted, coded, and analyzed using the IBM Corp. Released 2012. IBM SPSS Statistics for Windows, Version 21.0. Armonk, NY: IBM Corp.Results: The study included 702 females aged 18 years and older. 61.6% (n=432) of the participants consumed Trigonella foenum-graecum (fenugreek) and Foeniculum vulgare (fennel), whereas 45.7% (n=321) consumed soy products and flaxseeds. In addition, 44.7% (n=191) were diagnosed with breast tumors and other breast diseases. The association between the occurrence of breast conditions and the consumption of all the different types of phytoestrogen-rich plants in this study was significant, with a p-value of <0.001. (41.7%, n=187) diagnosed with uterine and ovarian-related conditions, the association with the consumption of fenugreek and fennel was significant with a p-value of <0.001, but for soy products and flaxseed consumption, the association was not significant with a p-value of 0.368. Regarding the consumer's attitudes and duration of consumption, most of them (41.0%, n=288) consume phytoestrogen-rich herbs and plants about every month during menstruation, and 55% (n=386) consumed phytoestrogen-rich plants a long time ago (for many years). About the purpose of consumption, 62.1% (n=436) answered to reduce pain and symptoms associated with menstruation. The majority of the participants (36.3%, n=255) have poor knowledge and don't know about the high estrogen levels' impact on their health. Conclusion:The study showed that there is a possible association between the consumption of some types of phytoestrogen-rich plants and the occurrence of several hormone-related tumors and diseases. Further studies are needed to evaluate the possible effects in consideration of the co-founding factors.

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Development of an estrogen-dependent breast cancer co-culture model as a tool for studying endocrine disruptors

Cited by 7 publications

References 34 publications

Collagen-Binding Nanoparticles for Paclitaxel Encapsulation and Breast Cancer Treatment

Collagen-Binding Nanoparticles for Paclitaxel Encapsulation and Breast Cancer Treatment

Application of an in Vitro Assay to Identify Chemicals That Increase Estradiol and Progesterone Synthesis and Are Potential Breast Cancer Risk Factors

Assessment of the Attitudes, Beliefs, and Potential Effects of Nutritional Phytoestrogenic Plant Consumption on Women’s Health in Saudi Arabia

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