Development of an EvaGreen-based real-time PCR assay for detection of Aleutian mink disease virus

Li, Li; Hu, Zhe; Sun, Jinhui; Guo, Kui; Chu, Xiaoyu; Wang, Xiaojun; Lu, Yixin

doi:10.1016/j.jviromet.2019.113751

Cited by 5 publications

(5 citation statements)

References 38 publications

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“…The CIEP was already used to identify and cull AMDV-positive animals in eradication programs [ 7 , 9 ], while the AMDVG ELISA was implemented to select AD-tolerant animals in conventional phenotypic selection plans [ 11 ]. While multiple high-throughput ELISA platforms have been developed for AD screening in mink farms with sensitivity and specificity of more than 96% and 97% [ 32 , 42 ], there are some rapid and highly sensitive diagnostic molecular methods, such as polymerase chain reaction (PCR), that can be used for AMDV detection and infection confirmation [ 43 , 44 ]. The main disadvantage of molecular techniques is that they have high sensitivity and specificity on samples taken from some specific organs, mainly spleen, that are not appropriate for high throughput screening and selection purposes [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…While multiple high-throughput ELISA platforms have been developed for AD screening in mink farms with sensitivity and specificity of more than 96% and 97% [ 32 , 42 ], there are some rapid and highly sensitive diagnostic molecular methods, such as polymerase chain reaction (PCR), that can be used for AMDV detection and infection confirmation [ 43 , 44 ]. The main disadvantage of molecular techniques is that they have high sensitivity and specificity on samples taken from some specific organs, mainly spleen, that are not appropriate for high throughput screening and selection purposes [ 43 , 44 ]. Meanwhile, ELISA tests can be simply performed on blood samples taken by nail clipping and are a better choice for regular farm screenings and selection strategies [ 32 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

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Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison)

Vahedi,

Ardestani,

Banabazi

et al. 2024

PLoS ONE

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Aleutian disease (AD) is a devastating infectious disease in American mink (Neogale vison) industry caused by Aleutian mink disease virus (AMDV). Two crucial steps toward controlling infectious diseases in farm animals are: (i) assessment of the infection risk factors to minimize the likelihood of infection and (ii) selection of animals with superior immune responses against pathogens to build tolerant farms. This study aimed to investigate AD risk factors and evaluate a novel “ImmunAD” approach for genetic improvement of AD tolerance. Phenotypic records and pedigree information of 1,366 and 24,633 animals were included in this study. The risk of animal’s age, sex, color type, and year of sampling on AMDV infection was assessed using a logistic regression model and counter immune-electrophoresis (CIEP) test results. ImmunAD phenotype was calculated based on AMDVG enzyme-linked immunosorbent assay (ELISA) and CIEP test results, and breeding values for ImmunAD were estimated using an animal model. Animals were classified into high-coordinated (HCIR), average-coordinated (ACIR), and low-coordinated immune responders (LCIR) using ImmunAD’s breeding values, and the impact of selection of HCIR on live grade of pelt quality (PQ), harvest weight (HW), and harvest length (HL) breeding values were evaluated. Age of > 1 year, male sex, and year of sampling were identified as significant risk factors of AD (p < 0.05). A moderate-to-high heritability (0.55±0.07) was estimated for ImmunAD, while a higher heritability was observed among the CIEP-positive animals (0.76±0.06). Significantly higher breeding values were observed for PQ and HL among HCIR than those for LCIR and ACIR (p < 0.05). Our findings indicate the critical role of male breeders in AD distribution within mink farms. Regular screening of AD in male breeders before pairing them with females during breeding seasons can help disease control. ImmunAD strategy can be applied to genetic improvement of AD tolerance, with favorable impacts on some growth and production traits. Higher genetic gains can be achieved in populations with higher AD seroprevalences.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison)

Vahedi,

Ardestani,

Banabazi

et al. 2024

PLoS ONE

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“…Interestingly, this approach was shown to have a greater detection rate compared to that of CIEP and was able to detect both American and Chinese strains of AMDV, in contrast to TaqMan based kits only recognizing American AMDV strains. Therefore, this method has been suggested to be a more reliable and specific approach to detect AMDV in mink [118]. In addition, loopmediated isothermal amplification (LAMP) has also been designed to recognize different regions of the VP2 gene of AMDV, and it was shown to outperform PCR test of AMDV positive mink, making it a rapid and reliable approach [119].…”

Section: Amdv Preventative Measuresmentioning

confidence: 99%

AMDV Vaccine: Challenges and Perspectives

Markarian

Abrahamyan

2021

Preprint

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Aleutian mink disease virus (AMDV) is known to cause the most significant disease in the mink industry. It is globally widespread and manifested as a deadly plasmacytosis and hyperglobulinemia. So far, measures to control viral spread have been limited to manual serological testing for AMDV-positive mink. Further, due to the persistent nature of this virus, attempts to eradicate Aleutian disease (AD) have largely failed. Therefore, effective strategies to control viral spread are of crucial importance for wildlife protection. One potentially key tool in the fight against this disease is by immunization of mink against AMDV. Throughout many years, several researchers have tried to develop AMDV vaccines and demonstrated varying degrees of protection in mink by those vaccines. Despite these attempts, there are currently no vaccines available against AMDV, allowing the continuation of the spread of Aleutian disease. Herein, we summarize previous AMDV immunization attempts in mink as well as other preventative measures with the purpose to shed light on future studies designing such a potentially crucial preventative tool against Aleutian disease.

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“…During the PCR process, the amount of the PCR product is monitored as fluorescence intensity in real time using non-specific double-stranded DNA-binding dyes or sequence-specific hybridization probes [ 10 ]. Non-specific double-stranded DNA-binding dyes include SYBR Green, SYTO9 [ 11 ], EvaGreen [ 12 ], and BrightGreen [ 13 , 14 ]. Dual-labeled hybridization probes (such as the TaqMan ® probe) have several advantages as sequence-specific hybridization probes, particularly with regard to specificity [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail

et al. 2022

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Quantitative real-time PCR (qPCR) is a powerful method for measuring nucleic acid levels and quantifying mRNA levels, even in single cells. In the present study, we compared the results of single-cell qPCR obtained by different quantification methods (relative and absolute) and different reverse transcription methods. In the experiments, we focused on the cerebral giant cell (CGC), a key neuron required for the acquisition of conditioned taste aversion in the pond snail Lymnaea stagnalis, and examined changes in the mRNA levels of 3 memory-related genes, cAMP-response element binding proteins (LymCREB1 and LymCREB2) and CREB-binding protein (LymCBP), during memory formation. The results obtained by relative quantification showed similar patterns for the 3 genes. For absolute quantification, reverse transcription was performed using 2 different methods: a mixture of oligo d(T) primers and random primers (RT method 1); and gene-specific primers (RT method 2). These methods yielded different results and did not show consistent changes related to conditioning. The mRNA levels in the samples prepared by RT method 2 were up to 3.3 times higher than those in samples prepared by RT method 1. These results suggest that for qPCR of single neurons, the efficacy and validity do not differ between relative and absolute quantification methods, but the reverse transcription step critically influences the results of mRNA quantification.

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Development of an EvaGreen-based real-time PCR assay for detection of Aleutian mink disease virus

Cited by 5 publications

References 38 publications

Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison)

Aleutian disease: Risk factors and ImmunAD strategy for genetic improvement of tolerance in American mink (Neogale vison)

AMDV Vaccine: Challenges and Perspectives

Comparison between relative and absolute quantitative real-time PCR applied to single-cell analyses: Transcriptional levels in a key neuron for long-term memory in the pond snail

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