Development of an evaporation-based microfluidic sample concentrator

Sharma, Nigel R.; Luk'yanov, A. M.; Bardell, Ron L.; Seifried, Lynn; Shen, Mingchao

doi:10.1117/12.764100

Cited by 16 publications

(23 citation statements)

References 6 publications

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“…The microfluidic device is designed to perform sweeping-gas membrane distillation, in which a fluid sample and a sweeping gas are separated by a hydrophobic porous membrane [26], [27]. The porous membrane prevents passage of the liquid as long as the contact angle is > 90°, but vapor generated as the aqueous sample is heated can pass through the membrane and into the sweeping gas stream.…”

Section: Methodsmentioning

confidence: 99%

“…Compared to other microfluidic techniques such as using trapped air pockets to capture vapor from a liquid stream [22], or using droplet traps [23], atomization [24], or a sheath flow [25] to expose a large liquid surface area to a gas flow, implementations of membrane distillation have significantly faster evaporation speed and greater sample capacity [21], [26]. In this work, we present a second-generation chip with a 5-fold improved evaporation rate (3.4 versus 0.65 mL/min for water at 100°C), increased scope of mobile phases (now compatible with practically any PET tracer), and an improved modular construction that enables the chip material to be matched with the PET tracer for optimal performance.…”

Section: Introductionmentioning

confidence: 99%

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Automatic concentration and reformulation of PET tracers via microfluidic membrane distillation

et al. 2017

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Short-lived radiolabeled tracers for positron emission tomography (PET) must be rapidly synthesized, purified, and formulated into injectable solution just prior to imaging. Current radiosynthesizers are generally designed for clinical use, and the HPLC purification and SPE formulation processes often result in a final volume that is too large for preclinical and emerging in vitro applications. Conventional technologies and techniques for reducing this volume tend to be slow, resulting in radioactive decay of the product, and often require manual handling of the radioactive materials. We present a fully-automated microfluidic system based on sweeping gas membrane distillation to rapidly perform the concentration and formulation process. After detailed characterization of the system, we demonstrate fast and efficient concentration and formulation of several PET tracers, evaluate residual solvent content to establish the safety of the formulated tracers for injection, and show that the formulated tracer can be used for in vivo imaging.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Automatic concentration and reformulation of PET tracers via microfluidic membrane distillation

et al. 2017

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“…We designed and fabricated a microfluidic chip (Figure 2) based on the principle of the design reported by Sharma et al 23 . In this approach, a porous hydrophobic membrane separates the liquid sample from a stream of dry gas.…”

Section: Methodsmentioning

confidence: 99%

“…The use of microfluidics for volume reduction has been explored by several groups 20–23 . The fastest approaches perform small scale membrane distillation 24 , with the sample on one side of a porous membrane and a solvent removal process (condensation or gas flow) on the other.…”

Section: Introductionmentioning

confidence: 99%

Compact microfluidic device for rapid concentration of PET tracers

Tseng

Dam

2014

Lab Chip

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HPLC purification and reformulation of positron emission tomography (PET) tracers can lead to significant dilution of the final product, making it difficult to produce a sufficiently high radioactivity concentration for some applications (e.g. small animal imaging, in vitro assays, and labelling of proteins with prosthetic groups). This is especially true for molecules with lengthy or low-yield syntheses. Starting the synthesis with more radioactivity increases the final radioactivity concentration but increases hazards and complexity of handling. An alternative is to concentrate the final product by a process such as rotary evaporation prior to downstream use. Because a rotovap requires significant space within a hot cell that could be put to more productive use, we developed a compact microfluidic system for concentration of PET tracers. This system also provides advantages in terms of repeatability, interfacing and potential for automation. We present here the design and performance characterization of the system, and demonstrate the concentration of several tracers in aqueous-based HPLC mobile phases.

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“…Sharma et al . 17 introduced a sample concentration device using convective gas flow for rapid isothermal evaporation of water. In their prototype, analyte concentration enhancement is achieved via fluid volume reduction.…”

Section: Introductionmentioning

confidence: 99%

Rapid point-of-care concentration of bacteria in a disposable microfluidic device using meniscus dragging effect

Zhang

Premasiri

et al. 2010

Lab Chip

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We report a low cost, disposable polymer microfluidic sample preparation device to perform rapid concentration of bacteria from liquid samples using enhanced evaporation targeted at downstream detection using surface enhanced Raman spectroscopy (SERS). The device is composed of a poly(dimethylsiloxane) (PDMS) liquid sample flow layer, a reusable metal airflow layer, and a porous PTFE (Teflon ™) membrane sandwiched in between the liquid and air layers. The concentration capacity of the device was successfully demonstrated with fluorescently tagged Escherichia coli (E. coli). The recovery concentration was above 85% for all initial concentrations lower than 1 × 104 CFU mL−1. In the lowest initial concentration cases, 100 μL initial volumes of bacteria solution at 100 CFU mL−1 were concentrated into 500 nL droplets with greater than 90% efficiency in 15 min. Subsequent tests with SERS on clinically relevant Methicillin-Sensitive Staphylococcus aureus (MSSA) after concentration in this device proved more than 100-fold enhancement in SERS signal intensity compared to the signal obtained from the unconcentrated sample. The concentration device is straightforward to design and use, and as such could be used in conjunction with a number of detection technologies.

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Development of an evaporation-based microfluidic sample concentrator

Cited by 16 publications

References 6 publications

Automatic concentration and reformulation of PET tracers via microfluidic membrane distillation

Automatic concentration and reformulation of PET tracers via microfluidic membrane distillation

Compact microfluidic device for rapid concentration of PET tracers

Rapid point-of-care concentration of bacteria in a disposable microfluidic device using meniscus dragging effect

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