Development of an HPLC method for the determination of nifedipine in human plasma by solid-phase extraction

Zendelovska, Dragica; Simeska, Suzana; Sibinovska, Olgica; Kostova, Elena; Miloševska, Kalina; Jakovski, Krume; Jovanovska, Emilija; Kikerkov, Igor; Trojacanec, Jasmina; Zafirov, Dimce

doi:10.1016/j.jchromb.2006.03.048

Cited by 32 publications

(23 citation statements)

References 13 publications

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“…Assay of the drugs have also been achieved by different spectroscopic methods [16][17][18][19][20][21][22][23], but the methods lack the desired sensitivity or involve tedious extraction step or use of organic solvents. Similarly, survey of literature for NIF revealed methods based on GC [24][25][26], different liquid chromatographic methods [27][28][29][30][31][32], and stripping voltammetry [33,34]. There are also some equilibrium visible spectrophotometric methods for NIF that are based on oxidation or reduction of the drug, where reactions products are colored [35][36][37][38].…”

Section: Introductionmentioning

confidence: 99%

A Kinetic spectrophotometric method for determination of amlodipine and Nifedipine in pharmaceutical preparations

Hemmateenejad

Miri

Kamali

2009

JICS

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A simple, accurate, sensitive and economical procedure for the estimation of amlodipine besylate and nifedipine, both in pure and dosage forms, has been developed. The method is based on the reduction of iron(III) by the studied drugs and subsequent interaction of iron(II) with ferricyanide to form Prussian blue. The reaction develops through a slow kinetics and completes in about 10 min. Both initial slope and fixed time methods were used to derive calibration graphs. The resulted calibration equations were linear in the concentration ranges of 1.0-20.0 and 3.0-19.0 ȝg ml -1 for AML and NIF, and the detection limits were 0.10 and 0.19 ȝg ml -1 , respectively. Seven replicate analyses of solutions containing three different levels of each drug resulted in very low relative error of prediction (less than 1.6%) and relative standard deviation (less than 4%) confirming accuracy and precision of the proposed method. The proposed method was applied to the determination of these drugs in pharmaceutical formulations and excellent recoveries were obtained.

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Section: Introductionmentioning

confidence: 99%

A Kinetic spectrophotometric method for determination of amlodipine and Nifedipine in pharmaceutical preparations

Hemmateenejad

Miri

Kamali

2009

JICS

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“…After that, molecular imprinting technique has undergone rapid development in 1990s. Because of the advantages over biopolymers, such as low cost, good physical and chemical stability and tailor-made selectivity for a target molecule, the MIPs materials have been widely applied in solid-phase extraction [16][17][18]. Given that nifedipine is a highly unstable compound, which is rapidly photo-degraded to its nitroso analogue when exposed to daylight [19] and its plasma protein binding rate reached 98%, on line solid-phase extraction is suitable for the pretreatment of plasma sample instead of liquid-liquid extraction and traditional off-line SPE.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a Novel Emulsion-Templated MIP Monolith and its Application for on Line Assay of Nifedipine in Human Plasma

Yang¹,

Liu²,

Liu³

et al. 2010

J Chromatograph Separat Techniq

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“…Several reported nifedipine quantification methods were either sensitive, but narrow in linearity ranges as HPLC-MS combining liquid-liquid extraction (Guo et al 2007;Wu et al 2007) and HPLC-MS-MS combining liquidliquid extraction Jan et al 1998) or solid-phase extraction (SPE) (Streel et al 1998), or wide in linearity range, but not sensitive enough to meet the requirement of pharmacokinetic studies as HPLC-UV combining liquid-liquid extraction (Hisham et al 2000;Shi et al 2008) or SPE (Ioannis et al 2003;Zendelovska et al 2006;Yritia et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Bioequivalence of nifedipine softgel and capsule in healthy Chinese volunteers by liquid chromatography–mass spectrometry

Zhang

Song

et al. 2010

Eur J Drug Metab Pharmacokinet

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The study aimed to compare and evaluate the bioequivalence of Calcigard-10 softgel and Adalat Ò 10 capsule in healthy Chinese volunteers in a randomized, two-way cross over study design with a washout period of 7 days. A sensitive and reproducible electro-spray ionization liquid chromatography-mass spectrometry (ESI-LC-MS) assay was developed and validated to determine nifedipine in human plasma using nitrendipine as internal standard. Nifedipine and nitrendipine were extracted from plasma using liquid-liquid extraction with methylene chloride as extraction solvent. The separation was performed by a Diamonsil ODS column (150 9 4.6 mm, 5 lm). The mobile phase was consisted of acetonitrile-5 mM ammonium acetate (52:48, v/v), delivered at flow rate of 1 mL/min. The 90% confidence intervals for the ratio values of logarithmic transformed C max and AUC were calculated to evaluate the bioequivalence of two preparations. The values of C max (92.3-112.7%), AUC 0-t (84.5-95.1%) and AUC 0-inf (84.4-95.5%) are within the interval criterion of 70-143% for C max and 80-125% for AUC. The Calcigard-10 softgel and Adalat Ò 10 capsule are bioequivalent.

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Development of an HPLC method for the determination of nifedipine in human plasma by solid-phase extraction

Cited by 32 publications

References 13 publications

A Kinetic spectrophotometric method for determination of amlodipine and Nifedipine in pharmaceutical preparations

A Kinetic spectrophotometric method for determination of amlodipine and Nifedipine in pharmaceutical preparations

Preparation of a Novel Emulsion-Templated MIP Monolith and its Application for on Line Assay of Nifedipine in Human Plasma

Bioequivalence of nifedipine softgel and capsule in healthy Chinese volunteers by liquid chromatography–mass spectrometry

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