Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

Abstract

In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cutoff value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.

Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken meat

“…A pure cell line that stably secreted anti-ENR antibody was selected through 3 rounds of subcloning, and then injected into the abdominal cavity of mice. One week later, ascites were prepared and purified using the saturated ammonium sulfate method, and the characteristics of the mAb were tested by ic-ELISA, as previously described (Fan et al, 2012;Li et al, 2018;Shen et al, 2019b).…”

Development of an ic-ELISA and colloidal gold strip for the detection of the beta-blocker carazolol

“…Fifteen female BALB/c mice (8-10 weeks old) were injected with different reaction specific antigens (CRZ-S1-BSA) and tested by ic-ELISA with different coating antigens (Li, Zhi, Jia, Hui, & Ai, 2017). Mice with the highest serum antibody titre and a half-maximal inhibitory concentration (IC 50 ) under optimal conditions were selected for cell fusion, as described previously (Chen et al, 2015).…”

Development of an immunochromatography assay for salinomycin and methyl salinomycin in honey

“…To prepare a control line (C-line) and a test line (T-line), 40 μL of goat anti-mouse antibody (1 mg/mL) and coated antigen (0.5 mg/mL SLM-EDC-BSA) were sprayed onto the NC membrane, and drying was maintained at 37°C for 2 h. The distance between them was 0.5 cm to ensure complete reaction of the reactants. A 3 mm wide test strip was cut with a cutter (Li, Zhi, Jia, Nie, & Ai, 2018). The gold-labeled antibody was immobilized on a solid support, as previously described (Briggs, Tapper, Sprosen, Mace, & Finch, 2017).…”