Development of an icELISA and Immunochromatographic Assay for Methyl-3-Quinoxaline-2-Carboxylic Acid Residues in Fish

Liu, Liqiang; Peng, Juan; Xie, Zhengjun; Song, Shanshan; Kuang, Hua; Xu, Chuanlai

doi:10.1007/s12161-017-0888-0

Cited by 18 publications

(6 citation statements)

References 31 publications

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“…The hapten was conjugated with KLH and OVA as the immunogen and coating antigen, respectively. The active ester method , was used to synthesize the immunogen (Azap–KLH) and coating antigen (Azap–OVA). Azap (1.9 mg) was dissolved in 400 μL of DMF with NHS (1.4 mg).…”

Section: Methodsmentioning

confidence: 99%

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

Lü

Cui

et al. 2020

ACS Omega

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In this study, a highly sensitive and specific monoclonal antibody (mAb) against azaperone was produced. The mAb belonged to the IgG2b subtype and its half maximal inhibitory concentration (IC 50 ) was 0.46 ng/mL. The limit of detection value was 0.081 ng/mL and the linear range (IC 20 −IC 80 ) was 0.165−1.29 ng/mL. Based on this anti-azaperone mAb, a colloidal gold immunochromatographic strip assay method was established for the detection of azaperone in pork and pork liver samples. For pork samples, the cutoff value of the colloidal gold immunochromatographic strip assay was 10 ng/g, and the cutoff value was also 10 ng/g in pig liver samples. This colloidal gold immunochromatographic strip assay is a rapid, simple, and practical method for the detection of azaperone in pork and pork liver samples available in markets.

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Section: Methodsmentioning

confidence: 99%

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

Lü

Cui

et al. 2020

ACS Omega

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“…In previous studies, this method was approved for the detection of many substances such as danofloxacin 14 and methyl-3-quinoxaline-2-carboxylic acid. 15 The present study aimed to develop an ICA for Kwa detection that is an effective method for the identification of PM and its differentiation from red Kwao Krua and other Pueraria spp. Moreover, the developed ICA exhibited a high sensitivity with a cutoff limit of 160 ng/mL, and this analytical procedure boasts advantages such as being simple and rapid.…”

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confidence: 99%

Immunochromatographic assay for the detection of kwakhurin and its application for the identification of Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham

Phaisan

Yusakul

Nuntawong

et al. 2020

Phytochemical Analysis

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Introduction: The plant Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham (PM), known by its common Thai name as white Kwao Krua, is sometimes misidentified because it presents similar botanical characteristics to those of Butea superba (red Kwao Krua). The phytochemicals in PM are phytoestrogens in the class of isoflavonoids, but Butea superba contains flavonoids that exhibit androgenic and antiestrogen effects.Objectives: This research aims to develop a simple analytical method for identification and to differentiate PM from red Kwao Krua and other Pueraria species.Methods: A gold nanoparticle-based immunochromatographic assay (ICA) was developed for the detection of kwakhurin (Kwa), a unique compound found in PM. The parameters, including sensitivity, accuracy, precision, and specificity, were validated. All samples were analyzed using ICA and high-performance liquid chromatography with UV detector (HPLC-UV). The results of the two methods were compared for consistency checking. Results:The cutoff limit of Kwa detection was 160 ng/mL, which was lower than in the HPLC-UV method. The repeatability and reproducibility of the ICA preparation and assembly showed high precision. The cross-reactivity to related isoflavonoids was less than 0.32%, which implied high specificity of the ICA for Kwa. Moreover, false-positive and false-negative results from other plant extracts were not observed. Conclusion:The developed ICA is applicable for distinguishing PM from red Kwao Krua and other Pueraria species. This simple analytical method can be applied for the identification of raw PM materials in the industrial and agricultural sectors.

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“…As both KIT and JOS are all small molecules (below 10 kDa), they cannot cause a mouse immune response directly, and therefore, need to be conjugated to a carrier protein to induce an immune response and produce antibodies against KIT (Liu et al, 2017). It is usual for small molecule compounds to be coupled to carrier proteins through active groups such as NH 2 or COOH.…”

Section: Hapten Design and Characterizationmentioning

confidence: 99%

Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples

Guo

Liu

Cui

et al. 2019

Food and Agricultural Immunology

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In this study, a kitasamycin (KIT) hapten was prepared through the ocarboxymethoxylamine method and used to produce a monoclonal antibody against KIT and josamycin (JOS). The half inhibition concentration for an indirect competitive enzyme-linked immunosorbent assay was 1.49 and 2.67 ng/mL, respectively. Based on this antibody, a gold immunochromatographic assay (GICA) was developed for the detection of KIT and JOS residues in milk and egg samples. A hand-held strip scanner was used for quantitative analysis, with limits of detection for KIT and JOS of 1.51 and 1.91 μg/L in milk, and 3.0 and 2.72 μg/kg in eggs. The proposed GICA was validated by LC-MS/MS, which indicated that the GICA method would be a useful tool for the on-site detection of KIT and JOS residues in milk and egg samples.

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Development of an icELISA and Immunochromatographic Assay for Methyl-3-Quinoxaline-2-Carboxylic Acid Residues in Fish

Cited by 18 publications

References 31 publications

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

Immunochromatographic assay for the detection of kwakhurin and its application for the identification of Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham

Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples

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