Development of an immunoaffinity chromatography column for selective extraction of a new agonist phenylethylamine A from feed, meat and liver samples

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

“…Immunoextraction columns have been used off-line with ultra-high performance LC (UHPLC) and MS/MS for the determination of aflatoxin M 1 in ice cream [49], and immunoaffinity columns were utilized off-line with LC-MS/MS for the analysis of multiple mycotoxins in food [50,51]. In addition, immunoextraction has been coupled both on-line and off-line with HPLC and fluorescence detection for the measurement of aflatoxins and mycotoxins in food [48,52,54], and used off-line with HPLC for the measurement of phenylethanolamine in animal feed and meat or liver samples [55]. Immobilized antibody fragments have been used to analyze the enantiomers of diarylalkyltriazole [56], and an immunoaffinity column has been used to separate ginsenoside epimers [57].…”

Multi-functional porous organic polymers for highly-efficient solid-phase extraction of β-agonists and β-blockers in milk

“…Due to the complexity of the composition of matrices and the trace amounts of analytes of interest, an effective extraction/ purication strategy prior to nal analysis is a prerequisite for determination of b-agonists and b-blockers. In recent years, various pretreatment techniques for the extraction of such residues from biological samples have been developed, including the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique, 5 solid phase extraction (SPE), [6][7][8] immunoaffinity chromatography (IAC) 9 and supercritical uid extraction (SFE). 10 Among these strategies, SPE has been the most frequently used method owing to several advantages: low reagent consumption and no requirement of expensive apparatus, 11,12 high sensitivity and good recovery with minimal sample transfer, [13][14][15][16] enhanced simplicity of operation with robustness, 17 and great safety with less exposure to toxic agents.…”

Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

“…Ommunoaffinity chromatography takes the advantage of specific and reversible interaction between antibody and the analyte, and enables selective extraction and enrichment of individual compounds or classes of compounds in one step (Şenyuva & Gilbert, 2010;Pichon & Combes, 2016). On the last decade, the application of immunoaffinity chromatography for a variety of samples pretreatment in mycotoxins (Longobardi et al, 2013;Marley et al, 2015;Wilcox et al, 2015;Zhang et al, 2016), veterinary drugs (Mei et al, 2014;Sun et al, 2016;Yang et al, 2016) and pesticides (Esteve-Turrillas et al, 2011;Xu et al, 2012) residues analyses have been reported.…”