Development of an immunoassay for the differentiation of menstrual blood from peripheral blood

Gray, Danielle; Frascione, Nunzianda; Daniel, Barbara

doi:10.1016/j.forsciint.2012.01.020

Cited by 16 publications

(5 citation statements)

References 33 publications

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“…36,37 An alternative approach using ELISA targeting MMP14, estrogen receptor α, and fibrinogen was used to differentiate between peripheral and menstrual blood, although no other body fluids were tested for cross reactivity. 38…”

Section: Vaginal Secretions and Menstrual Bloodmentioning

confidence: 99%

Forensic body fluid identification: state of the art

Harbison¹,

Fleming²

2016

RRFMS

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Body fluid identification is a key component in the forensic scientists' tool box and has been carried out both at the crime scene and in the laboratory for many years. Historically, methods relied on (bio) chemical-based tests, many of which lacked specificity. In this review, current technologies for identifying body fluids are described including the use of RNA (mRNA and miRNA), epigenetics, spectroscopic techniques such as Raman spectroscopy and microspectrophotometry, biosensors, and immunochromatographic methods which are outlined alongside their strengths and weaknesses. The potential for new insights into the identification of cells from new technologies such as massively parallel sequencing is explored.

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Section: Vaginal Secretions and Menstrual Bloodmentioning

confidence: 99%

Forensic body fluid identification: state of the art

Harbison¹,

Fleming²

2016

RRFMS

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“…Information about the composition of each body fluid is summarized in Table 1, which shows the major components of PB, MB and vaginal fluid [32][33][34][35][36][37][38][39]. Each body fluid has a unique composition, what provides a strong support for the hypothesis that the identification of the body fluids can be achieved using Raman spectroscopy [40].…”

Section: Comparison Of Peripheral and Menstrual Bloodmentioning

confidence: 99%

Raman spectroscopy coupled with advanced statistics for differentiating menstrual and peripheral blood

Sikirzhytskaya

Sikirzhytski

Lednev

2012

Journal of Biophotonics

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Body fluids are a common and important type of forensic evidence. In particular, the identification of menstrual blood stains is often a key step during the investigation of rape cases. Here, we report on the application of near-infrared Raman microspectroscopy for differentiating menstrual blood from peripheral blood. We observed that the menstrual and peripheral blood samples have similar but distinct Raman spectra. Advanced statistical analysis of the multiple Raman spectra that were automatically (Raman mapping) acquired from the 40 dried blood stains (20 donors for each group) allowed us to build classification model with maximum (100%) sensitivity and specificity. We also demonstrated that despite certain common constituents, menstrual blood can be readily distinguished from vaginal fluid. All of the classification models were verified using cross-validation methods. The proposed method overcomes the problems associated with currently used biochemical methods, which are destructive, time consuming and expensive.

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“…Most confirmatory methods are disadvantageous due to their complex and destructive nature, such as the Takayama crystal test for blood, are not applicable to multiple body fluids, and can be labor-intensive (Greaves, 1932;Johnston, Newman, & Frappier, 2003). Identification of vaginal material and distinguishing between circulatory and menstrual blood is currently difficult due to their mixed composition (Divall, 1984;Divall & Ismail, 1983;Gray, Frascione, & Daniel, 2012). For more information regarding conventional body fluid identification methods in forensic science, see the review by Virkler & Lednev, 2009. Depending on the context of the case, some stains should be comprehensively assessed for all potential body fluids to reduce bias (Fleming & Harbison, 2010).…”

Section: Conventional Body Fluid Identification Methodsmentioning

confidence: 99%

A review of direct polymerase chain reaction of DNA and RNA for forensic purposes

Lynch

Fleming

2019

WIREs Forensic Science

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Short tandem repeat (STR) profiling is frequently carried out on biological stains in forensic casework, and the application of messenger RNA (mRNA) body fluid identification is becoming more prevalent. Increasing constraints on analysis time, resources, an increase in the number of samples for analysis, and a large backlog of cases creates significant demand for quicker methods that direct polymerase chain reaction (PCR) can help overcome. Direct PCR bypasses DNA and/or RNA extraction, and in some cases, quantification by adding the sample or substrate punch to the amplification reaction. This method is currently used in some laboratories on reference samples. Direct PCR is also a component of portable devices, which could improve efficiency even further by producing results at the scene, and enabling triaging of samples for further testing. Portable devices are currently used for STR profiling of reference samples but not for mRNA profiling. Various concerns regarding the reliability of the method and equipment (portable devices) must first be addressed before incorporation into forensic casework. This review discusses the limitations and factors that need to be considered for direct PCR to be used for the analysis of casework samples, including being used for mRNA body fluid identification. This article is categorized under: Forensic Biology > Forensic DNA Technologies Forensic Biology > Body Fluid Identification Forensic Biology > Applications of RNA

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Development of an immunoassay for the differentiation of menstrual blood from peripheral blood

Cited by 16 publications

References 33 publications

Forensic body fluid identification: state of the art

Forensic body fluid identification: state of the art

Raman spectroscopy coupled with advanced statistics for differentiating menstrual and peripheral blood

A review of direct polymerase chain reaction of DNA and RNA for forensic purposes

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