Development of an immunochromatographic assay for rapid detection of clorprenaline in pig urine

Abstract

A clorprenaline (CLP) monoclonal antibody, 4G1, raised against the CLP-hapten was successfully prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic assays for the detection of CLP in pig urine samples. The half maximal inhibitory concentration of the antibody was 0.435 ng/mL, and the limit of detection was 0.104 ng/mL. The linear range was 0.104-1.818 ng/ mL, and cross-reactivity with the CLP analogue was <5%. In the spiked sample and recovery test, the recovery ranged from 81.8% to 100.5%, indicating that the ic-ELISA was suitable for CLP analysis in pig urine. The critical value of the immunochromatographic strip in pig urine was 10 ng/mL and could be used for semi-quantitative analysis of CLP. Thus, this immunochromatographic strip was suitable for quickly and sensitively detecting CLP residues in pig urine samples. ARTICLE HISTORY

Development of an immunochromatography assay for salinomycin and methyl salinomycin in honey

“…The glass instruments required for the experiment were soaked in aqua regia and washed several times with ultra-pure water. Colloidal gold nanoparticles with a diameter of 40 nm were prepared according to the classical sodium citrate reduction method frequently used in our previous work (Jiang, Zeng, Liu, Song, & Kuang, 2018). First, 100 mL of chloroauric acid (0.01 g/L) was heated.…”

“…Then 1 mL of blocking buffer (0.5% casein, w/v) was added, and the solution was stirred for 2 h. Next, the mixture was centrifuged at 8,000 × g for 25 min to remove the blocking agent and excess antibody. The precipitate was collected and washed with gold-labeled resuspension buffer three times (20 mM Tris [pH 8.2], 0.1% PEG, 0.1% Tween, 5% sucrose, 5% trehalose, 0.2% BSA, and 5% Brij) (Jiang, Zeng, et al, 2018). Finally, the mAb was reconstituted to a volume of 1 mL with gold-labeled resuspension buffer including 0.02% NaN 3 and stored at 4°C until use (Kong, Xie et al 2017;Xie et al, 2018).…”

Ultrasensitive anti-melamine monoclonal antibody and its use in the development of an immunochromatographic strip

“…Melamine in dairy products has been listed as a compulsory inspection item. Because of the large number of tests needed, traditional detection methods have become very costly, and because of the increasing workload, they cannot meet current demands (Jiang, Zeng, Liu, Song, & Kuang, 2018a). China urgently needs to carry out high-throughput, rapid detection methods for the detection of melamine residues.…”