Development of an immunochromatographic strip assay for ractopamine detection using an ultrasensitive monoclonal antibody

Abstract

A sensitive gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed for the detection of ractopamine (RCT), a beta-adrenergic agonist. The immunogen and coating antigen were synthesized by the carbodiimide method and conjugated with keyhole limpet hemocyanin and ovalbumin, respectively. The highly sensitive and specific monoclonal antibody was prepared for RCT, with a 50% inhibition concentration of 0.05 ng/ml and had no cross-reactivity with other beta-adrenergic agonists. An ultrasensitive and rapid immunochromatographic strip assay was developed with an RCT cutoff value of 2 ng/ml. Both developed methods can be used for RCT detection in swine urine.

Development of an immunochromatographic assay for hexestrol and diethylstilbestrol residues in milk

“…The anti-HES mAb was labeled with GNPs according to the methods previously described by Gu, Liu, Song, Kuang, and Xu (2015), Khaemba et al (2015) and with minimal modifications. The procedure was as follows: 40 μl of 0.1 M K 2 CO 3 was mixed together with 10 ml GNPs and the ion strength of the mixture was adjusted to pH 8.2.…”

Development of a monoclonal antibody assay and a lateral flow strip test for the detection of paromomycin residues in food matrices

“…The enzyme-linked immunosorbent assay (ELISA) and the lateral flow strip test allow the semi-quantitative analysis of various drug residues and have the advantages of simplicity, rapidity, specificity, convenience, high sensitivity, and cost effectiveness, and the samples need only be pretreated simply before detection (Gu, Liu, Song, Kuang, & Xu, 2016;Peng, Song, Liu, Kuang, & Xu, 2016;Wang et al, 2016;Yu, Liu, Song, Kuang, & Xu, 2016).…”

Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs

“…The coating antigen was prepared by coupling FF-HS with OVA (or BSA) using an active ester method (Gu, Liu, Song, Kuang, & Xu, 2016;Suryoprabowo, Liu, Peng, Kuang, & Xu, 2015). Fifteen milligrams of FF-HS, 18 mg of EDC, and 11 mg of NHS were dissolved in 2 mL of 50% N,N-dimethylformamide, and the mixture was stirred for 4 h at room temperature (solution A).…”