Development of an Immunochromatographic Test Strip for Rapid Simultaneous Detection of Enrofloxacin and Ofloxacin in Tissue of Chicken Muscle and Pork

Wu, Yue; Guo, Shuang; Dong, Qian; Yu-zhen, Song

doi:10.1007/s12161-016-0474-x

Cited by 31 publications

(9 citation statements)

References 18 publications

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“…Lateral flow assay (LFA) has attracted much attention due to its advantages of simplicity, rapidity, and portability; moreover it is economical and has no need for highly skilled personnel (Pan et al, 2018). This popular detection method has been widely used for foodborne pathogens (Hwang et al, 2016;Song et al, 2016), heavy metal ions (Liu et al, 2012), hormones (Yang et al, 2015), and antibiotics (Wu et al, 2016). The most common method of LFA is gold nanoparticle (AuNP)-LFA (Ang et al, 2015;Pawade et al, 2016), but LFA of colored nanoparticles is often limited by low sensitivity, so that it is necessary to develop a technique with high sensitivity and low cost (Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

An aptamer-exonuclease III (Exo III)–assisted amplification-based lateral flow assay for sensitive detection of Escherichia coli O157:H7 in milk

Ren

Gao

Yang

et al. 2021

Journal of Dairy Science

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Escherichia coli O157:H7 (E. coli O157:H7), one of the most widespread foodborne pathogens, can cause a series of diseases and even lead to death. In this study, a highly sensitive method was developed by combining aptamer-exonuclease III (Exo III)-assisted amplification with lateral flow assay (LFA) based on gold nanoparticles (AuNP). The compound of single-stranded (ss) DNA-anti-E. coli O157:H7 aptamer (ssDNA-aptamer) was formed by hybridization between designed target ssDNA and aptamer. When E. coli O157:H7 was present, target bacteria were bound with the aptamer, and the free target ssDNA was hybridized with the probes of the designed hairpin (HP) structure. Exo III digests the 3′ double-stranded blunt end of the complex and releases the enzyme product. Because the remaining sequence of the HP of the designed enzyme product was the same as the target ssDNA sequence, the target ssDNA could be amplified. Finally, the enhanced target ssDNA was combined with AuNP-LFA to achieve visual detection of E. coli O157:H7. The quantitative ability of this platform for E. coli O157:H7 was 7.6 × 10 1 cfu/mL in pure culture, and the detection limit in milk was 8.35 × 10 2 cfu/mL. This LFA was highly specific to E. coli O157:H7, and the time for detection of E. coli O157:H7 in milk was 4 h. Hence, this system has important application prospects in the detection of pathogenic bacteria in dairy products.

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Section: Introductionmentioning

confidence: 99%

An aptamer-exonuclease III (Exo III)–assisted amplification-based lateral flow assay for sensitive detection of Escherichia coli O157:H7 in milk

Ren

Gao

Yang

et al. 2021

Journal of Dairy Science

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“…X. Chen et al reported the indirect simultaneous competitive immunoassay screening for marbofloxacin and OFL residues with half maximal inhibitory concentration (IC 50 ) of 0.76 ± 0.12 ng/mL for marbofloxacin and 2.70 ± 0.28 ng/mL for OFL [19]. Y. Wu et al screened an anti-enrofloxacin and OFL monoclonal antibody (mAb) with IC 50 of 6.67 and 7.13 ng/ mL, respectively, and developed an immunochromatographic assay with decision limits of 0.089 and 0.217 ng/mL [26]. B.N.…”

Section: Introductionmentioning

confidence: 99%

Gold Nanoparticle-Based Paper Sensor for Highly Specific Detection of Ofloxacin in Beef

Oliveira¹,

Soares²,

Teixeira³

et al. 2019

J Food Chem Nanotechnol

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Ofloxacin (OFL), a third-generation fluoroquinolone, is widely used as an antibacterial drug to prevent diseases in livestock and poultry. In this study, a paper sensor based on gold nanoparticle and a highly specific monoclonal antibody (mAb) against OFL was prepared to detect OFL residues in beef. The limit of detection of the developed immunochromatographic strip for OFL reached 0.16 ng/mL, and the linear range was from 3.125 ng/mL to 100 ng/mL, with the following optimal parameters for the preparation of colloidal gold-labeled mAb probe: pH 6.5, 2 mg/mL OFL-bovine serum albumin antigen, 10 µg/mL antibody, and 12 min immunoreaction time. Cross-reactivity (CR) experiment indicated that the developed strip is highly specific and features low CR with fluoroquinolone analogues. Overall, the developed strip is reliable for the rapid detection of OFL in beef and can be considered an effective screening method for food safety and quality management.

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“…Enrofloxacin (ENR) is a broad spectrum of fluoroquinolone (FQ) antibiotic with high physiological activity and among the most consumed quinolones on livestock in China [1,2]. The widely used quinolones will then accumulate in the food chain, causing environment pollution and would possibly lead to undesired effects in humans [3,4].…”

Section: Introductionmentioning

confidence: 99%

The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin

Ma¹

2018

JCEBE

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In this paper, the fluorescence spectra of Enrofloxacin (ENR) in different pH conditions was studied in order to determine its structural changes due to protonation with pH changes. The ENR two-step dissociation constant is calculated and the fluorescence quantum yield under acidic conditions is measured. In the strong acidic conditions, ENR exists of H 3 L 2+ form of which maximum emission wavelength is at 450 nm. At the condition of pH 2.45 to 4.23, ENR exists of H 2 L + form with strong and steady fluorescence. The maximum emission wavelength is still 450 nm. At the condition of pH more than 4.23, the maximum emission wavelengths are gradually blue shifted to 445 nm and the fluorescence intensity decrease with the increase of pH which shows that H 2 L + loses one proton with the increase of pH and exists in the form of bipolar ion HL. When the pH is more than 12.28, the fluorescence intensity are weakened to nearly disappear with the increase of pH value, indicating that HL gradually loses the proton with the conversion to the anion of Lwhich is weaker fluorescence. In the buffer solution of pH 3.00, with quinine sulfate as reference, the fluorescence quantum yield of ENR at excitation wavelength of 274 nm is 0.125.

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Development of an Immunochromatographic Test Strip for Rapid Simultaneous Detection of Enrofloxacin and Ofloxacin in Tissue of Chicken Muscle and Pork

Cited by 31 publications

References 18 publications

An aptamer-exonuclease III (Exo III)–assisted amplification-based lateral flow assay for sensitive detection of Escherichia coli O157:H7 in milk

An aptamer-exonuclease III (Exo III)–assisted amplification-based lateral flow assay for sensitive detection of Escherichia coli O157:H7 in milk

Gold Nanoparticle-Based Paper Sensor for Highly Specific Detection of Ofloxacin in Beef

The Investigation of Fluorescence Spectra and Fluorescence Quantum Yield of Enrofloxacin

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