Development of an immunoenzyme test system for diagnosis if rabies

Chernov, A. N.; Ефимова, М. А.; Makaev, Haris N.; Nikitin, Andrey I.; Авзалова, А Ф; Kalmikova, Yuliya V.; Faizov, T.H.

doi:10.15562/bmj.v6i2.511

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2024

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“…It is known from the analyzed scientific sources that the basis of IFA is test-systems, with the help of which, if the reaction is carried out correctly, it is possible to obtain true results and make reasonable conclusions. To date, test systems for the detection of endotoxins of gramnegative bacteria have not been developed among known pathogens, so it is not possible to detect them using the IFA method [1,8,14].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the significance of immunoenzyme test-system for determining antiendotoxic immunity against the endotoxin of gram negative bacteria

Nuralieva,

Ruzmetov

2024

BIO Web Conf.

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The purpose of the study was to determine the specificity and sensitivity of the immunoenzymatic test system that detects antibodies against the antigens of these bacteria in order to evaluate antiendotoxic immunity against GShPB endotoxins. It was found that flat bottom polystyrene solid phase carrier was selected for the proposed experimental test system for the detection of endotoxins of these bacteria by IFA method. The sensitivity of the recommended test system was 75% in colon dysbiosis, 76% in acute tonsillitis, 89% in acute bronchitis, and 95% in urinary tract infections, and the specificity was 5-25%, thus the diagnostic value of this test system it was proved for the first time.

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Section: Introductionmentioning

confidence: 99%