2009
DOI: 10.1128/cvi.00305-08
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Development of an Immunoglobulin M Capture-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Acute Infections withBartonella henselae

Abstract: We describe the development of an immunoglobulin M-specific enzyme-linked immunosorbent assay for the detection of an early antibody response to Bartonella henselae, the causative agent of cat scratch disease, bacillary angiomatosis, and endocarditis. This assay discriminates between B. henselae-positive and -negative patient samples with sensitivity and specificity values of 100% and 97.1%, respectively.

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Cited by 19 publications
(13 citation statements)
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“…According to ELISA assays for B. henselae infection, recombinant proteins rGroES, rRplL, rBepA and rGroEL yielded high sensitivity 4 70%, but low specificity 59% (Table 3). Only the B. henselae r17-kDa protein has high sensitivity and specificity (Loa et al, 2006;McCool et al, 2008;Hoey et al, 2009) (Table 3).…”
Section: Discussionmentioning
confidence: 99%
“…According to ELISA assays for B. henselae infection, recombinant proteins rGroES, rRplL, rBepA and rGroEL yielded high sensitivity 4 70%, but low specificity 59% (Table 3). Only the B. henselae r17-kDa protein has high sensitivity and specificity (Loa et al, 2006;McCool et al, 2008;Hoey et al, 2009) (Table 3).…”
Section: Discussionmentioning
confidence: 99%
“…In that case, the 17-kDa recombinant protein was examined in Western blot assays and shown to have reactivity with human sera from patients with CSD, very similar to the IFA (99). The recombinant 17-kDa antigen has subsequently been adapted to an ELISA-based assay to detect IgG antibody against B. henselae (100) and for an IgM capture assay where high sensitivity and specificity were reported (101). Another approach to serologic diagnosis is to employ subcellular fractions as an antigen in an ELISA to detect IgG for the diagnosis of CSD (102).…”
Section: Serologymentioning
confidence: 99%
“…Since the isolation of Bartonella spp. is difficult, serologic tests are commonly used for etiologic diagnosis of cat-scratch disease (Vermeulen et al;, Hoey et al, 2009.…”
mentioning
confidence: 99%