2022
DOI: 10.3390/cimb44080248
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Development of an Improved Method for the Isolation and Culture of Newborn Sheep Primary Hepatocytes

Abstract: The liver plays a crucial role in metabolism, synthesis, biotransformation, secretion, and excretion. Hepatocytes are the main cells of the liver and can be used as a cell model to study liver function. The classic method of collagenase perfusion to isolate hepatocytes is a two-step technique that is time-consuming, labor-intensive, and has high technical requirements. Therefore, in this study, we compared different methods for isolating and culturing primary hepatocytes. We found that the 0.25% trypsin and 0.… Show more

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Cited by 2 publications
(4 citation statements)
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“…The culture and identification of sheep primary hepatocytes were performed as previously described [16]. Briefly, primary hepatocytes presented with irregular polygon morphology after adherent growth.…”
Section: Cell Culture and Identificationmentioning
confidence: 99%
See 3 more Smart Citations
“…The culture and identification of sheep primary hepatocytes were performed as previously described [16]. Briefly, primary hepatocytes presented with irregular polygon morphology after adherent growth.…”
Section: Cell Culture and Identificationmentioning
confidence: 99%
“…After periodic acid-Schiff staining for glycogen and assessment of alpha-fetoprotein (AFP) expression, the isolated cells were identified as hepatocytes using the detection of hepatocyte-specific markers (cytokeratin (CK)-18 and albumin). Cell purity was at least 95% based on CK-18, AFP, and albumin staining [16].…”
Section: Cell Culture and Identificationmentioning
confidence: 99%
See 2 more Smart Citations