Development of an in situ root caries model. A. In vitro investigations

Smith, Philip W.; Preston, K.P.; Higham, Susan

doi:10.1016/j.jdent.2004.10.020

Cited by 23 publications

(31 citation statements)

References 33 publications

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“…Perhaps more so than for enamel, dentin remineralization is heavily influenced by the presence of fluoride. Several researchers have shown that fluoride can lead to surface hypermineralization Mukai et al, 2001] but not necessarily arrest as even hypermineralized dentin lesions can be remineralized as shown presently (subsurface lesions) or previously [Mukai and ten Cate, 2002;Smith et al, 2005]. Differences between tissues were observed presently, however not for ΔΔZ but for ΔL.…”

Section: Discussionmentioning

confidence: 80%

“…Previous studies [Smith et al, 2005;Moron et al, 2013] have shown that different conditions during lesion creation (e.g. degree of saturation with respect to hydroxyapatite, presence of fluoride) can lead to dentin lesions with different mineral distributions.…”

Section: Discussionmentioning

confidence: 99%

“…However, comparatively few such studies were conducted on root caries lesions as researchers have generally focused on lesion creation under different conditions [Shellis, 2010;Moron et al, 2013] or the study of fluoride and/or potentially cariostatic agents on de-and remineralization of one particular lesion type in situ [Wefel et al, 1995[Wefel et al, , 2002 and in vitro [Xie et al, 2008;Qia et al, 2012]. Nonetheless, a series of mechanistic studies [Kawasaki et al, 2000;Smith et al, 2005;Preston et al, 2008] found that lesion baseline mineral distribution affects fluoride response under subsequent remineralization, that smaller lesions are more prone to remineralization and that, as shown for enamel [ten Cate and Duijsters, 1982], the mineral and fluoride content of the surface layer determines the rate of remineralization. Furthermore, dentin lesions were shown to be less susceptible to remineralization than their enamel counterparts, that comparatively more fluoride is necessary to induce remineralization in dentin lesions [ten Cate et al, 1995], that lesions progress faster in dentin than in enamel [Arends et al, 1992] and that, unlike for enamel, mineral deposition is mainly driven by lesion-bound, rather than ambient, fluoride [Preston et al, 2008].…”

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Effect of Lesion Baseline Severity and Mineral Distribution on Remineralization and Progression of Human and Bovine Dentin Caries Lesions

2015

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The aims of this laboratory study were to compare the effects of lesion baseline severity, mineral distribution and substrate on remineralization and progression of caries lesions created in root dentin. Lesions were formed in dentin specimens prepared from human and bovine dentin using three protocols, each utilizing three demineralization periods to create lesions of different mineral distributions (subsurface, moderate softening, extreme softening) and severity within each lesion type. Lesions were then either remineralized or demineralized further and analyzed using transverse microradiography. At lesion baseline, no differences were found between human and bovine dentin for integrated mineral loss (ΔZ). Differences in mineral distribution between lesion types were apparent. Human dentin lesions were more prone to secondary demineralization (ΔΔZ) than bovine dentin lesions, although there were no differences in ΔL. Likewise, smaller lesions were more susceptible to secondary demineralization than larger ones. Subsurface lesions were more acid-resistant than moderately and extremely softened lesions. After remineralization, differences between human and bovine dentin lesions were not apparent for ΔΔZ although bovine dentin lesions showed greater reduction in lesion depth L. For lesion types, responsiveness to remineralization (ΔΔZ) was in the order extremely softened > moderately softened > subsurface. More demineralized lesions exhibited greater remineralization than shallower ones. In summary, some differences exist between human and bovine dentin and their relative responsiveness to de- and remineralization. These differences, however, were overshadowed by the effects of lesion baseline mineral distribution and severity. Thus, bovine dentin appears to be a suitable substitute for human dentin in mechanistic root caries studies.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

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Effect of Lesion Baseline Severity and Mineral Distribution on Remineralization and Progression of Human and Bovine Dentin Caries Lesions

2015

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“…The considerable current interest in the use of in situ caries models has been discussed in a companion paper, 1 which describes studies that examined the effects of different preparatory regimes upon remineralisation of artificial sub-surface root dentinal lesions. This paper describes a pilot experiment in which the in situ model used to study coronal caries in the authors' laboratory was adapted, based on the findings of preliminary in vitro investigations, to study dentinal caries.…”

Section: Introductionmentioning

confidence: 99%

“…1 In view of the findings in that paper, that the pattern of remineralisation of lesions produced by exposure to a demineralising buffer solution for 2 and 5 days differed-principally due to the higher mineral content of the surface layer in the 5 day lesions-it was decided in this experiment to attempt to re-investigate this finding in situ. Methods Volunteers were recruited after ethical approval was obtained, and after informed consent forms were signed.…”

Section: Introductionmentioning

confidence: 99%