Development of an in vitro skin sensitization test using human cell lines: The human Cell Line Activation Test (h-CLAT)

Ashikaga, Takao; Yoshida, Yukiko; Hirota, Morihiko; Yoneyama, Kelly Aparecida Geraldo; Itagaki, Hiroshi; Sakaguchi, Hitoshi; Miyazawa, Masaaki; Itô, Yûichi; Suzuki, Hiroyuki; Toyoda, Hidekazu

doi:10.1016/j.tiv.2005.10.012

Cited by 278 publications

(139 citation statements)

References 14 publications

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“…Therefore, we are now evaluating the sensitizing properties of metal ions by an in vitro method using human monocytic cells. 21,22 The information provided from this research may be utilized as an important starting point to estimate the allergenic properties of metallic biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Release and Accumulation of Metal Ions from Titanium and Nickel by Accelerated Dissolution Test in Simulated Body Environments

et al. 2015

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To study the allergenic properties of titanium (Ti), the corrosion behaviors and following metal ions release of Ti and nickel (Ni) in simulated physiological environments were investigated. Anodic polarization tests and accelerated dissolution tests were performed in artificial saliva with and without fluorine, and in artificial sweat. Ti showed good corrosion resistance in the artificial saliva without fluorine even when the passive film was physically destroyed by mechanical wear. The concentration of Ti ions released into the solution showed relatively higher values in artificial saliva with fluorine and in artificial sweat, however, it remained to be below 10 ppm. On the other hand, Ni was not passivated in these solutions and the concentrations of the released Ni ions were about 100 ppm. Thus, Ti can be regarded as a considerably safer material than Ni from the viewpoint of corrosion engineering. The large differences in the corrosion characteristics and metal ion solubility between Ti and Ni under the experimental conditions are discussed.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Release and Accumulation of Metal Ions from Titanium and Nickel by Accelerated Dissolution Test in Simulated Body Environments

et al. 2015

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“…One of the last in vitro methods to be accepted by the OECD is the In Vitro Skin Sensitisation or human cell activation test (h-CLAT), which is based on activation of the human monocytic leukaemia cell line THP-1 [27] as developed previously [28]. Other were accepted in 2015, including Test 442C, the In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA) [29], which was developed some years ago [30,31] and Test 442D: In Vitro Skin Sensitisation: ARE-Nrf2 Luciferase Test Method [32].…”

Section: Dermal Absorptionmentioning

confidence: 99%

Alternative Methods to Animal Testing for the Safety Evaluation of Cosmetic Ingredients: An Overview

Vinardell

Mitjans

2017

Cosmetics

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Abstract:The safety of cosmetics sold in Europe is based on the safety evaluation of each individual ingredient conducted by those responsible for putting the product on the market. However, those substances for which some concern exists with respect to human health (e.g., colorants, preservatives, UV-filters, nanomaterials) are evaluated at the European Commission level by a scientific committee, currently called the Scientific Committee on Consumer Safety (SCCS). According to the Cosmetics Regulation (European Commission, 2009), it is prohibited in the European Union (EU) to market cosmetic products and ingredients that have been tested on animals. However, the results of studies performed before the ban continue to be accepted. In the current study, we evaluated the use of in vitro methods in the dossiers submitted to the SCCS in the period between 2013 and 2016 based on the published reports issued by the scientific committee, which provides a scientific opinion on these dossiers. The results of this evaluation were compared with those of an evaluation conducted four years previously. We found that, despite a slight increase in the number of studies performed in vitro, the majority of studies submitted to the SCCS is still done principally in vivo and correspond to studies performed before the ban.

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“…Several non-animal predictive methods have been developed to reduce animal experimentation used for chemical testing including computational approaches to integrate data from different test platforms for hazard identification as recently reviewed (Ezendam et al, 2016). Three test methods for skin sensitization are accepted as test guidelines at the OECD; the ARE-NRF2 luciferase method (KeratinoSens™) assay (Andreas et al, 2011;Natsch and Emter, 2008), the Direct Peptide Reactivity Assay (DPRA) (Gerberick et al, 2004) and the human Cell Line Activation Test (h-CLAT) (Ashikaga et al, 2006). In addition to hazard identification, information on skin sensitizer potency is imperative in order to allow quantitative risk assessment and to define exposure limits.…”

Section: Cells and Flow Cytometrymentioning

confidence: 99%

The GARD platform for potency assessment of skin sensitizing chemicals_suppl

2017

ALTEX

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Development of an in vitro skin sensitization test using human cell lines: The human Cell Line Activation Test (h-CLAT)

Cited by 278 publications

References 14 publications

Evaluation of Release and Accumulation of Metal Ions from Titanium and Nickel by Accelerated Dissolution Test in Simulated Body Environments

Evaluation of Release and Accumulation of Metal Ions from Titanium and Nickel by Accelerated Dissolution Test in Simulated Body Environments

Alternative Methods to Animal Testing for the Safety Evaluation of Cosmetic Ingredients: An Overview

The GARD platform for potency assessment of skin sensitizing chemicals_suppl

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