Development of an infusion bioreactor for the accelerated preparation of decellularized skeletal muscle scaffolds

Abstract

The implantation of decellularized tissue has shown effectiveness as a strategy for the treatment of volumetric muscle loss (VML) injuries. The preparation of decellularized tissue typically relies on the diffusion driven removal of cellular debris. For bulky tissues like muscle, the process can be lengthy, which introduces opportunities for both tissue contamination and degradation of key ECM molecules. In this study we report on the accelerated preparation of decellularized skeletal muscle (DSM) scaffolds using a infusion system and examine scaffold performance for the repair of VML injuries. The preparation of DSM scaffolds using infusion was dramatically accelerated. As the infusion rate (1% SDS) was increased from 0.1 to 1 and 10ml/hr, the time needed to remove intracellular myoglobin and actin decreased from a maximum of 140±3hrs to 45±3hrs and 10±2hrs respectively. Although infusion appeared to remove cellular debris more aggressively, it did not significantly decrease the collagen or glycosaminoglycan composition of DSM samples when compared to un-infused controls. Infusion prepared DSM samples retained the aligned network structure and mechanical integrity of control samples. Infusion prepared DSM samples supported the attachment and in-vitro proliferation of myoblast cells and was well tolerated by the host when examined in-vivo.

Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications

“…To the best of our knowledge, this is the first report of whole urethra decellularization, including the RBS layer. Several groups have performed skeletal muscle decellularization of tissue fragments 21 39 40 . We used porcine urethras that are an excellent animal model to study and evaluate treatments for sphincter deficiencies due to its functional similarities with the human urethra 4 5 .…”

Recovery from volumetric muscle loss injury: A comparison between young and aged rats

“…Our multidisciplinary team is exploring the development of implantable biomaterials targeting the repair of damaged skeletal muscle (Hurd and others 2015; Kasukonis and others 2016). Towards that end we are particularly interested in understanding the response of muscle to volumetric muscle loss injury (Corona and others 2015; Grogan and others 2011).…”

Codelivery of Infusion Decellularized Skeletal Muscle with Minced Muscle Autografts Improved Recovery from Volumetric Muscle Loss Injury in a Rat Model

“…To prepare DSM samples for implantation, whole tibialis anterior (TA) muscle was infusion decellularized using the previously reported 16 infusion system (Fig. 1).…”

“…To improve the preparation of DSM scaffolds, our group has recently reported on a novel infusion method, which can accelerate the decellularization process, without compromising the physical, chemical, and biocompatibility properties. 16 Our goal is to explore the utility of these infusion prepared DSM scaffolds for the repair of VML injuries. Yet, the implantation of DSM scaffolds alone, particularly when explored in the lower extremity, appears to offer an incomplete intervention for the repair of VML injuries, includes combining ECM scaffolds with the appropriate progenitor cells.…”