Development of an Integrated Bioprocess for production of potential Malaria vaccines with Pichia pastoris

Sequential/parallel production of potential Malaria vaccines – A direct way from single batch to quasi-continuous integrated production

“…An off-line screening of five parameters with an AEKTA TM purifier 100 found two critical EBA-parameters, namely the pH and Imidazole concentration of the sample and the running buffer. These were optimized in 12 runs of the integrated EBA production procedure (Borchert et al, 2013).…”