Development of an Integrated Chip for Automatic Tracking and Positioning Manipulation for Single Cell Lysis

Young, Chao-Wang; Hsieh, J. C.; Ay, Chyung

doi:10.3390/s120302400

Cited by 8 publications

(6 citation statements)

References 10 publications

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“…Numerous researchers are working on developing a non-invasive separation device to characterize cells based on dielectric parameters 16 . DEP has been widely used in cell lysis research 17 and in characterisation of a variety of yeasts 18 and human cells, such as nerve cells 19 , platelets 20 , sperm cells 21 , cancer cells 22 , and leukaemia cells 23 . Many studies have been conducted to determine the dielectric properties of human whole blood 24 , T and B lymphocytes 25,26 , and erythrocytes 27 .…”

Section: Introductionmentioning

confidence: 99%

Determination of electrophysiological properties of human monocytes and THP-1 cells by dielectrophoresis

Mohamed¹,

Razak²,

Kadri³

2019

Biomed. Res. Ther.

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Introduction: Dielectrophoresis (DEP) is based on polarization and bioparticle movement in applied electric fields. Each type of cells has its own electrical properties within the DEP spectra and undergoes significant changes in direction of frequency following an increase of an applied nonuniform alternating current (AC) electric field. Therefore, DEP can be an effective technique for characterization and separation of different cells. The goal of the current study was to determine the electrophysiological properties of human monocytes and a human monocytic cell line originated from an acute monocytic leukemia patient (THP-1), using a lab on a chip (LOC) device that utilised microarray dot electrodes. Methods: Ten microliters of human monocytes isolated from peripheral blood mononuclear cells and highly confluent THP-1 cells were diluted in DEP medium and added to the spacer of the LOC device. Subsequently, the AC signal in the range of 10 kHz to 2 MHz was supplied to the LOC device, and the dynamic behaviours of monocytes and THP-1 cells due to the DEP force were observed. Images were captured and analyzed using MATLAB software. Results: Microscopic visualization showed that THP-1 cells were physically larger than human monocytes. The dielectric parameters (radius, diameter, and conductivity and permittivity of the cytoplasm and membrane) were greater for THP-1 cells compared to monocytes. The cross-over frequencies for THP-1 cells and monocytes were respectively 66 kHz and 280 kHz. Conclusion: In conclusion, the DEP spectra reflected the morphological differences between monocytes and THP-1 cells. The dielectric properties for each type of cells can be used as the criteria in the development of DEP-based characterization assays.

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Section: Introductionmentioning

confidence: 99%

Determination of electrophysiological properties of human monocytes and THP-1 cells by dielectrophoresis

Mohamed¹,

Razak²,

Kadri³

2019

Biomed. Res. Ther.

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“…They observed cell lysis at a rate of 0.2 cells per second. Young et al . reported an on‐chip electrical lysis of single‐lymphoma cell at 80 % success rate and injection‐to‐lysis time of 60 s. Bahi and co‐workers trapped an array of single‐cells via on‐chip DEP (1 V) followed by lysis at 60 V potential.…”

Section: Single‐cell Lysismentioning

confidence: 99%

“…The cell traveled to aT -intersection where the high electric field (4 kV) ruptures the cell.T hey observed cell lysis at ar ate of 0.2 cellsp er second. Young et al [152] reported an onchip electrical lysis of single-lymphoma cell at 80 %s uccess rate and injection-to-lysis time of 60 s. Bahi and co-workers [153] trapped an array of single-cells via on-chip DEP (1 V) followed by lysis at 60 Vp otential. The method reported 100 %t rapping and lysing efficiency.H owever,t his method did not provide a way for separate collection of each single-cell lysates and all the extracted RNA was collected using ap ipette after lysis.…”

Section: Single-cell Lysismentioning

confidence: 99%

Microfluidic Devices in the Fast‐Growing Domain of Single‐Cell Analysis

Khan

Mao

et al. 2018

Chemistry A European J

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Recent advances in cellular analysis revealed that the seemingly identical cells are heterogeneous in term of functionality, compositions, and genetic performance. These differences cause difficulty in the diagnostic for a specific model of disease. Detection of biomolecules such as DNA, RNA, and protein or analysis of cell(s), detection of cell surface molecules, and secreted protein, can help us to improve the understanding of a targeted disease and development of new diagnostic and therapeutic approaches. A single-cell includes the minute quantity of these target molecules. Microfluidic devices have the ability to capture a single-cell and its lysate into a pico or femtoliter volumes droplet, or micro-well thus preventing dilution and limiting diffusion. In this review we described the advancement and limitations in microfluidic techniques used toward single-cells analysis.

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“…The authors found single cell lysing events occurring at a rate of 0.2 cells s-1. Young and co-workers 208 demonstrated a microfluidic device for EOF positioning and electrical lysis of a single lymphoma cell. Their method reported a success rate of 80% while taking approximately 60 seconds on average from cell injection to cell lysis.…”

Section: Sample Preparationmentioning

confidence: 99%