Development of an ISSR based SCAR marker to identify small cardamom Malabar (prostrate panicle) variety (Elettaria cardamomum (L.) Maton.)

Jose, Sherin Mariya; K, Mary Mathew; Cs, Anisha; Sasidharan, Swapna; Ys, Rao

doi:10.1016/j.scienta.2021.110777

Cited by 6 publications

(1 citation statement)

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“…An array of molecular marker technologies has made substantial contributions to the discrimination of plant varieties, assessment of genetic diversity and relationship, and construction of genetic maps (L. Chen et al., 2005, 2007; P. J. Wang, Fang et al., 2022). Notably, RAPD (random‐amplified polymorphic DNA), amplified fragment length polymorphism, inter simple sequence repeat, and simple sequence repeat markers have been widely applied to tea plants (Barut et al., 2020; Fang et al., 2012; Jose et al., 2022; Sharma et al., 2022), effectively enabling the evaluation of genetic diversity of tea germplasm resources. As a third‐generation molecular marker technology, single nucleotide polymorphism (SNP) stands out as a biomarker of choice for its wide distribution in plant genomes (Buckler & Thornsberry, 2002; Fang et al., 2014).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation of tea plant in Ningde and its adjacent regions revealed by single nucleotide polymorphism genotyping

Zhu,

Fan,

et al. 2023

Agronomy Journal

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Ningde, (ND) situated in China's prominent tea‐producing region, is recognized as the origin of white tea. It also boasts a wealth of premium tea germplasm resources, which are of vital importance in tea breeding programs. In this investigation, utilizing nanofluidic single nucleotide polymorphisms (SNP) genotyping technology, the genetic diversity and population structure of tea germplasms sourced from ND and its surrounding regions were scrutinized. A comprehensive collection of 101 tea plant germplasm accessions sourced from ND and its neighboring regions was acquired. Our analysis identified 75 stable and reliable SNP loci that demonstrated the rich genetic diversity of the ND tea germplasms, with an average observed heterozygosity (Ho) of 0.411 and expected heterozygosity of 0.382. Notably, the multiple analyses based on high‐quality SNPs revealed that the wild‐type landraces of tea plants in ND are genetically distinct from the cultivars. The analysis of molecular variance results suggested that intrapopulation variation contributed to 79% of the total genetic variation. Moreover, tea plant germplasms in ND interacted with the genetic material of landraces in Nanping and Lishui. Overall, our study provides essential insights into the genetic diversity, relationship, and conservation of ND tea germplasms, and lays a molecular basis for innovative utilization and breeding improvement of tea plant germplasms.This article is protected by copyright. All rights reserved

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