The reliable detection of ammonia at room temperature is crucial for not only maintaining environmental safety but also for reducing the risks of hazardous pollutants. In this study, the electrochemical modification of laser-induced graphene (LIG) with polyaniline (PANI) led to the development of a chemo-resistive nanocomposite (PANI@LIG) for detecting ammonia levels at room temperature. The composite is characterized by field emission scanning electron microscopy, Fourier transforms infrared, and Raman and X-ray photoelectron spectroscopy. This work marks the first utilization of PANI@LIG for gas sensing and introduces a simple but effective approach for fabricating low-cost wearable gas sensors with high sensitivity and flexibility.