Development of an oil-sealed anaerobic fermentation process for high production of γ-aminobutyric acid with Lactobacillus brevis isolated by directional colorimetric screening

Cell-free systems for biosynthesis: towards a sustainable and economical approach

“…In metabolic channels, the information is transferred within either temporarily or permanently formed enzyme complexes or nanocompartments through the local diffusion of small messenger molecules. To systematically elucidate the Bio-electricity, 73,74 ω-hydroxy acids 81 Bio-cellulose, 54 bio-ethanol, 3 unnatural base pair 64 Bio-ethanol, 10 p-hydroxy styrene 80 Unnatural L-4-nitro-Trp amino acid, 39 minimal cells 40 Bacterial cellulose, 53,159 γ-aminobutyric acid 160 mechanisms of metabolic channelling within enzymes and enzyme complexes through modelling, several efforts have been made, for example, with the development of the 'channel by proximity model', a straightforward approach where the active sites of enzymes are brought in close proximity. 165 Co-immobilization is another advanced approach relying upon the immobilization of multiple enzymes involved in a cascade of reactions, either onto a common solid support (organic, inorganic, or biopolymer) or by direct crosslinking.…”