Development of an on-line analytical platform to screen haptens in shuxuening injection based on high-performance liquid chromatography coupled with ion-trap multistage mass spectrometry and human serum albumin fluorescence detection

Niu, Yanyan; Wang, Hong; Tong, Xin; Li, Xiang; Guo, Ning; Zhang, Xiaotian; Luo, Yukun; Gao, Yaning; Tian, He; Lin, Zongtao; Chen, Shizhong

doi:10.1016/j.chroma.2019.04.011

Cited by 9 publications

(2 citation statements)

References 29 publications

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“…The mobile phase was composed of water containing 0.1% formic acid (A) and methanol (B), and the gradient of eluent B started at 5% and gradually increased to 90% over 90 min at a flow rate of 1 mL/min. The MS conditions refer to Niu et al [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives

Tong

Wang

Shen

et al. 2021

JoF

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The main active ingredients of the fruiting bodies of Shiraia bambusicola and Rubroshiraia bambusae are Hypocrellins, belonging perylenequinones with potential photodynamic activity against cancer and microbial diseases. However, the strains of S. bambusicola and R. bambusae do not produce hypocrellins in culture, so resource exploitation of natural products was seriously restricted. In this study, a series of novel Shiraia-like fungal endophyte strains, with varying sporulation ability and synthesizing diverse secondary metabolites, was isolated from different bamboos. Based on phylogenetic analyses and morphological characteristics of the endophytes, Pseudoshiraia conidialis gen. et sp. nov. is proposed. The secondary metabolites of different fruiting bodies and strains have been comprehensively analyzed for the first time, finding that the endophytic strains are shown not only to produce hypocrellins, but also other perylenequinonoid compounds. It was noteworthy that the highest yield of total perylenequinone production and hypocrellin A appeared in P. conidialis CNUCC 1353PR (1410.13 mg/L), which was significantly higher than any other wild type P. conidialis strains in published reports. In view of these results, the identification of Shiraia-like endophytes not only confirm the phylogenetic status of similar strains, but will further assist in developing the production of valuable natural products.

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Section: Methodsmentioning

confidence: 99%

Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives

Tong

Wang

Shen

et al. 2021

JoF

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“…It is made from the extract of Ginkgo biloba L. and used to treat ischemic cerebrovascular disease, coronary heart disease, cerebral thrombosis, and cerebral vasospasm in Chinese medical practice. [1][2][3][4][5] Previous studies have revealed that the main active ingredients in Ginkgo biloba L. are flavonoids and ginkgolides, [6][7][8][9][10][11] which are officially set to evaluate the quality of SXNI. High performance liquid chromatography (HPLC) is officially regulated to determine the content of active ingredients in SXNI.…”

Section: Introductionmentioning

confidence: 99%

Towards real time release testing of Shuxuening injection based on near infrared spectroscopy and accuracy profile

Ouyang

Zhan

Tang

et al. 2022

Journal of Near Infrared Spectroscopy

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Real time release testing (RTRT) has been applied in the pharmaceutical process to ensure the high quality of finished products. Near infrared (NIR) spectroscopy is one of the primary analytical methods to implement RTRT. In this study, an NIR quantitative method was developed to determine the content of total flavonol glycosides in Shuxuening injection and validated by the accuracy profile approach. Combining the NIR validation with quality specification limits, a reliable RTRT method was constructed. Shuxuening injection samples of different concentrations were prepared and characterized by NIR spectroscopy. A first-order Savitzky–Golay derivative was used to pretreat the NIR spectra, and the competitive adaptive reweighted sampling method was used to select the feature variables. Partial least squares (PLS) regression was used to build the NIR quantitative model. The trueness, precision, and accuracy of the developed NIR models were validated by accuracy profile, and the measurement uncertainty was also estimated. Finally, the unreliability graph as a decision tool was established to avoid risk, enabling correct decision making to release of Shuxuening injection. The root mean square error of calibration, root mean square error of cross validation, root mean square error of prediction, and the ratio of prediction to deviation of the PLS model were 19.6 μg·mL−1, 20.9 μg·mL−1, 29.9 μg·mL−1, and 12.2, respectively, indicating the NIR quantitative model had good predictive performance. The validation results prove that the precision, trueness, and accuracy of the NIR quantitative model were within the acceptable limits. Based on the unreliability graph, the decision to release Shuxuening injection was satisfied, if the prediction of total flavonol glycosides fell into the range from 783 μg·mL−1 to 900 μg·mL−1. The RTRT method for Shuxuening injection based on NIR spectroscopy and accuracy profile can improve the efficiency and accuracy of quality control.

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Chromatographic Separation with On-Line (Bio)Assay, a Rapid Technique for Screening Active Compounds from Chinese Medicines

Li,

Fan,

2024

Quality Control of Chinese Medicines

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Development of an on-line analytical platform to screen haptens in shuxuening injection based on high-performance liquid chromatography coupled with ion-trap multistage mass spectrometry and human serum albumin fluorescence detection

Cited by 9 publications

References 29 publications

Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives

Phylogenetic Position of Shiraia-Like Endophytes on Bamboos and the Diverse Biosynthesis of Hypocrellin and Hypocrellin Derivatives

Towards real time release testing of Shuxuening injection based on near infrared spectroscopy and accuracy profile

Chromatographic Separation with On-Line (Bio)Assay, a Rapid Technique for Screening Active Compounds from Chinese Medicines

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