Development of an optical biosensor using gold nanoparticles and quantum dots for the detection of Porcine Reproductive and Respiratory Syndrome Virus

Stringer, R. Cody; Schommer, Susan K.; Hoehn, Daniel; Grant, Sheila

doi:10.1016/j.snb.2008.05.018

Cited by 58 publications

(50 citation statements)

References 15 publications

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“…There are hosts of biomolecular interactions/processes that are studied using QD energy transfer formats and these include nucleic acid interactions, binding protein conformation changes, antibody binding, aptamer interactions, and protease cleavage [283,513,[515][516][517][518]524,554]. Assay formats found in conjunction with QD FRET are quite varied and include (i) cleavage-based assays (e. g., proteases, kinases, and DNAzymes) (Figure 6.27) [321,365,368,[526][527][528][529][556][557][558], (ii) conformational change-based assays (e.g., aptamers, binding proteins, and DNA molecular beacons) [518,[559][560][561], (iii) displacement assays (e.g., antibodies and binding proteins) (Figure 6.28) [554,562], (iv) various immunoassays (including direct, displacement, and sandwich) [367,[562][563][564], (v) nucleic acid hybridization [518,555], and (vi) assays based on acceptor spectral changes (mainly pH or ion sensitive dyes) (Figure 6.16) [79, 527,556]. Given the unique photophysical properties of QDs, our increasing fundamental understanding of these unique materials when used in energy transfer configurations, and the availability of improved synthesis and bioconjugation methods, we can expect continued utilization in many FRETbased biological assays, with increasing emphasis on multiplexed and in vivo detection [321,…”

Section: Semiconductor Nanocrystalsmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

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Section: Semiconductor Nanocrystalsmentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

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“…This can occur over distances of 10-100 Å with the most efficient transfer occurring at 20-60 Å . Therefore; FRET has extensive used in the study of macromolecular interactions, such as protein-protein association, antibody-antigen binding and DNA hybridization (Jamiesona et al 2007;Mattoussi et al 2004;Smith et al 2008;Stringer et al 2008;Xu et al 2006). In this study, FRET was used to provide evidence supporting the accurate antibody orientation on the QD surface.…”

Section: Fluorescence Resonance Energy Transfer Techniquementioning

confidence: 99%

Investigation of photosensitively bioconjugated targeted quantum dots for the labeling of Cu/Zn superoxide dismutase in fixed cells and tissue sections

Say

Kılıç

Özcan

et al. 2011

Histochem Cell Biol

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This study presents the development of targeted and antibody cross-linked QDs and explores whether these bioconjugates could specifically and effectively label Cu/Zn superoxide dismutase (SOD1) on fixed cells and tissues. QD-antibody conjugation was achieved by using our previously invented AmiNoacid (monomer) Decorated and Light Underpining Conjugation Approach (ANADOLUCA) method. In this method, we have used a photosensitive aminoacid monomer having ruthenium complex which is a synthetic and inexpensive material for the preparation of bioconjugates. Its specificity was demonstrated by extracting the active enzyme from rat liver lysate by using the bioconjugate. It provided accurate antibody orientation, high specificity and mechanic stability. The protocol steps for QD-antibody conjugation and specimen preparation were described in detail. The nanobioconjugates were prepared under mild conditions (for example in day light), independent of pH and temperature, without affecting conformation and function of protein. This protocol is simple, inexpensive and can be successfully adapted to detect other targets on different cell types and tissues.

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“…Generally for the diagnosis of the disease, Ab based biosensors are preferred (Conroy et al, 2009). Mostly, the sensors are designed to diagnose the disease of veterinary importance as well as having zoonotic importance and vice versa (Stringer et al, 2008;Tran et al, 2012). Some have developed the sensors for surrogate human viruses so as to avoid the direct contact with the human viruses (Connelly et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Nanodiagnostics: a new frontier for veterinary and medical sciences

Lambe¹,

Minakshi²,

Brar³

et al. 2016

JEBAS

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Infectious diseases are one of the greatest threats to animal and human population living in the developing world. These diseases have capacity to instigate in a small area and then open out very fast to the rest of the world and causing a heavy pandemic situation, for example; avian influenza pandemic. Such diseases infect large masses of population and may lead to loss of lives and also incur huge economic losses. Therefore, the best way to control these diseases is by diagnosing it at a very primary level and taking necessary precautionary measures so as to avoid the spread. Since last few years, the diagnostic approach has changed from tedious molecular biological techniques, to easy and rapid diagnostic techniques. Nanotechnology has extended the molecular diagnostics limit to nanoscale. These developed techniques do not require sophisticated laboratories and expert personnel, and hence are a cheap diagnostic approach. These assays can also be performed at the field level where the patient is present and get the results there itself. Hence, they are also called as pen side test or lab on chip diagnostic assays. The biological tests using nanotechnology become quicker, more flexible and more sensitive. These techniques have greatly influenced the diagnostic approach in the veterinary as well as medical field. Especially in the developing countries such as India, where the laboratory services are not

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Development of an optical biosensor using gold nanoparticles and quantum dots for the detection of Porcine Reproductive and Respiratory Syndrome Virus

Cited by 58 publications

References 15 publications

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Investigation of photosensitively bioconjugated targeted quantum dots for the labeling of Cu/Zn superoxide dismutase in fixed cells and tissue sections

Nanodiagnostics: a new frontier for veterinary and medical sciences

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